Inhibin beta A Antibody (CL15357) - Azide and BSA Free

Novus Biologicals | Catalog # NBP3-44161

Novus Biologicals
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Key Product Details

Validated by

Orthogonal Validation

Species Reactivity

Human, Rat

Applications

Immunohistochemistry-Paraffin, Western Blot

Label

Unconjugated

Antibody Source

Monoclonal Mouse IgG1 Clone # CL15357

Format

Azide and BSA Free
View all INHBA Primary Antibodies »

Product Specifications

Immunogen

This antibody was generated using a recombinant protein sequence of P08476, with the exact immunogen sequence remaining proprietary.

Clonality

Monoclonal

Host

Mouse

Isotype

IgG1

Scientific Data Images for Inhibin beta A Antibody (CL15357) - Azide and BSA Free

Inhibin beta A Antibody (CL15357) - Azide and BSA Free Immunohistochemistry-Paraffin: Inhibin beta A Antibody [NBP3-44161]

Immunohistochemistry-Paraffin: Inhibin beta A Antibody [NBP3-44161]

Analysis in human placenta and kidney tissues using NBP3-44161 antibody. Corresponding Inhibin beta A RNA-seq data are presented for the same tissues.
Inhibin beta A Antibody (CL15357) - Azide and BSA Free Immunohistochemistry-Paraffin: Inhibin beta A Antibody [NBP3-44161]

Immunohistochemistry-Paraffin: Inhibin beta A Antibody [NBP3-44161]

Staining of human kidney shows no positivity in cells in tubules as expected.
Inhibin beta A Antibody (CL15357) - Azide and BSA Free Immunohistochemistry-Paraffin: Inhibin beta A Antibody [NBP3-44161]

Immunohistochemistry-Paraffin: Inhibin beta A Antibody [NBP3-44161]

Staining of human cerebral cortex shows weak to moderate cytoplasmic positivity in a subset of neurons.
Inhibin beta A Antibody (CL15357) - Azide and BSA Free Immunohistochemistry-Paraffin: Inhibin beta A Antibody [NBP3-44161]

Immunohistochemistry-Paraffin: Inhibin beta A Antibody [NBP3-44161]

Staining of human prostate shows no positivity in glandular cells as expected.
Inhibin beta A Antibody (CL15357) - Azide and BSA Free Immunohistochemistry-Paraffin: Inhibin beta A Antibody [NBP3-44161]

Immunohistochemistry-Paraffin: Inhibin beta A Antibody [NBP3-44161]

Staining of human placenta shows strong cytoplasmic positivity in trophoblastic cells.
Inhibin beta A Antibody (CL15357) - Azide and BSA Free Immunohistochemistry-Paraffin: Inhibin beta A Antibody [NBP3-44161]

Immunohistochemistry-Paraffin: Inhibin beta A Antibody [NBP3-44161]

Staining of rat cerebral cortex shows moderate cytoplasmic positivity in a subset of neurons in the upper layers.
Inhibin beta A Antibody (CL15357) - Azide and BSA Free Western Blot: Inhibin beta A Antibody [NBP3-44161]

Western Blot: Inhibin beta A Antibody [NBP3-44161]

Analysis in human cell line U-138MG.

Applications for Inhibin beta A Antibody (CL15357) - Azide and BSA Free

Application
Recommended Usage

Immunohistochemistry-Paraffin

1:50 - 1:200

Western Blot

1 ug/ml
Application Notes
For IHC-Paraffin, HIER pH 6 retrieval is recommended.

Formulation, Preparation, and Storage

Purification

Protein A purified

Reconstitution

Centrifuge the vial of lyophilized antibody at 12,000 x g for 20 seconds. Reconstitute by adding sterile, distilled water to achieve a final antibody concentration of 1mg/ml.

Formulation

Lyophilized from a 0.2 um filtered solution in PBS with Trehalose

Format

Azide and BSA Free

Preservative

No Preservative

Concentration

LYOPH mg/ml

Shipping

The product is shipped at ambient temperature. Upon receipt, store it immediately at the temperature recommended below.

Stability & Storage

Store at 4C short term. Aliquot and store at -20C long term. Avoid freeze-thaw cycles.

Calculators

The reconstitution calculator allows you to quickly calculate the volume of a reagent to reconstitute your vial. Simply enter the mass of reagent and the target concentration and the calculator will determine the rest.

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Background: INHBA

The inhibin beta A subunit joins the alpha subunit to form a pituitary FSH secretion inhibitor. Inhibin has been shown to regulate gonadal stromal cell proliferation negatively and to have tumor-suppressor activity. In addition, serum levels of inhibin have been shown to reflect the size of granulosa-cell tumors and can therefore be used as a marker for primary as well as recurrent disease. Because expression in gonadal and various extragonadal tissues may vary severalfold in a tissue-specific fashion, it is proposed that inhibin may be both a growth/differentiation factor and a hormone. Furthermore, the beta A subunit forms a homodimer, activin A, and also joins with a beta B subunit to form a heterodimer, activin AB, both of which stimulate FSH secretion. Finally, it has been shown that the beta A subunit mRNA is identical to the erythroid differentiation factor subunit mRNA and that only one gene for this mRNA exists in the human genome.

Long Name

Inhibin Subunit Beta A

Alternate Names

Activin Beta A, Inhibin Beta A, Inhibin Subunit Beta A

Gene Symbol

INHBA

Additional INHBA Products

Product Documents for Inhibin beta A Antibody (CL15357) - Azide and BSA Free

Certificate of Analysis

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Product Specific Notices for Inhibin beta A Antibody (CL15357) - Azide and BSA Free

This product is for research use only and is not approved for use in humans or in clinical diagnosis. Primary Antibodies are guaranteed for 1 year from date of receipt.

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Protocols

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FAQs

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