Insect Cathepsin L Antibody

Catalog # Availability Size / Price Qty
MAB22591
MAB22591-SP
Product Details
Citations (7)
FAQs
Supplemental Products
Reviews (1)

Insect Cathepsin L Antibody Summary

Species Reactivity
Insect
Specificity
Detects pro and mature forms of Insect Cathepsin L in direct ELISAs and Western blots. In Western blots, less than 5% cross-reactivity with recombinant human Cathepsin L is observed and no cross-reactivity with recombinant mouse Cathepsin L is observed.
Source
Monoclonal Mouse IgG1 Clone # 193702
Purification
Protein A or G purified from hybridoma culture supernatant
Immunogen
S. frugiperda ovarian cell line Sf 21-derived Insect Cathepsin L
Formulation
Lyophilized from a 0.2 μm filtered solution in PBS with Trehalose. *Small pack size (SP) is supplied either lyophilized or as a 0.2 µm filtered solution in PBS.

Applications

Recommended Concentration
Sample
Western Blot
1 µg/mL

Recombinant Insect Cathepsin L

Immunoprecipitation
25 µg/mL
Conditioned cell culture medium spiked with Recombinant Insect Cathepsin L, see our available Western blot detection antibodies

Please Note: Optimal dilutions should be determined by each laboratory for each application. General Protocols are available in the Technical Information section on our website.

Reconstitution Calculator

Reconstitution Calculator

The reconstitution calculator allows you to quickly calculate the volume of a reagent to reconstitute your vial. Simply enter the mass of reagent and the target concentration and the calculator will determine the rest.

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Preparation and Storage

Reconstitution
Reconstitute at 0.5 mg/mL in sterile PBS.
Reconstitution Buffer Available
Reconstitution Buffer 1 (PBS)
Catalog #
Availability
Size / Price
Qty
RB01
Shipping
The product is shipped at ambient temperature. Upon receipt, store it immediately at the temperature recommended below. *Small pack size (SP) is shipped with polar packs. Upon receipt, store it immediately at -20 to -70 °C
Stability & Storage
Use a manual defrost freezer and avoid repeated freeze-thaw cycles.
  • 12 months from date of receipt, -20 to -70 °C as supplied.
  • 1 month, 2 to 8 °C under sterile conditions after reconstitution.
  • 6 months, -20 to -70 °C under sterile conditions after reconstitution.

Background: Cathepsin L

The pro form of insect cell Cathepsin L is stable at neutral pH, but autoactivates in acidic buffers under reducing conditions. The activated enzyme is similar in substrate specificity to mammalian Cathepsin L, and is very active as a protease, as judged by its ability to degrade azocasein. Cystatins A, B, C, E/M, F, SA, and SN are potent inhibitors of the insect cell Cathepsin L. Like other cysteine proteases related to papain, insect cell Cathepsin L is also inhibited by E-64 and reagents which react with thiols.

Entrez Gene IDs
1514 (Human); 13039 (Mouse); 118276442 (Insect)
Alternate Names
Cathepsin L; cathepsin L1; CATL; CTSL; CTSL1; CTSLEC 3.4.22.15; EC 3.4.22; FLJ31037; Major excreted protein; MEP

Product Datasheets

Citations for Insect Cathepsin L Antibody

R&D Systems personnel manually curate a database that contains references using R&D Systems products. The data collected includes not only links to publications in PubMed, but also provides information about sample types, species, and experimental conditions.

7 Citations: Showing 1 - 7
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  1. Integrated analysis of the aging brain transcriptome and proteome in tauopathy
    Authors: CG Mangleburg, T Wu, HK Yalamanchi, C Guo, YC Hsieh, DM Duong, EB Dammer, PL De Jager, NT Seyfried, Z Liu, JM Shulman
    Mol Neurodegener, 2020;15(1):56.
    Species: Drosophila
    Sample Types: Tissue Homogenates
    Applications: Western Blot
  2. VAMP associated proteins are required for autophagic and lysosomal degradation by promoting a PtdIns4P-mediated endosomal pathway
    Authors: D Mao, G Lin, B Tepe, Z Zuo, KL Tan, M Senturk, S Zhang, BR Arenkiel, M Sardiello, HJ Bellen
    Autophagy, 2019;0(0):.
    Species: Human
    Sample Types: Whole Tissue
    Applications: IHC
  3. Lysosomal Machinery Drives Extracellular Acidification to Direct Non-apoptotic Cell Death
    Authors: AA Mondragon, A Yalonetska, AJ Ortega, Y Zhang, O Naranjo, J Elguero, WS Chung, K McCall
    Cell Rep, 2019;27(1):11-19.e3.
    Species: Insect - Drosophila
    Sample Types: Whole Tissue
    Applications: IHC
  4. Ubiquilins regulate autophagic flux through mTOR signalling and lysosomal acidification
    Authors: M ?entürk, G Lin, Z Zuo, D Mao, E Watson, AG Mikos, HJ Bellen
    Nat. Cell Biol., 2019;21(3):384-396.
    Species: Insect
    Sample Types: Cell Lysates
    Applications: Western Blot
  5. Spermine synthase deficiency causes lysosomal dysfunction and oxidative stress in models of Snyder-Robinson syndrome
    Authors: C Li, JM Brazill, S Liu, C Bello, Y Zhu, M Morimoto, L Cascio, R Pauly, Z Diaz-Perez, MCV Malicdan, H Wang, L Boccuto, CE Schwartz, WA Gahl, CF Boerkoel, RG Zhai
    Nat Commun, 2017;8(1):1257.
    Species: Drosophila
    Sample Types: Tissue Homogenates
    Applications: Western Blot
  6. The unique GGA clathrin adaptor of Drosophila melanogaster is not essential.
    Authors: Luan S, Ilvarsonn A, Eissenberg J
    PLoS ONE, 2012;7(9):e45163.
    Species: Drosophila
    Sample Types: Tissue Homogenates
    Applications: Western Blot
  7. Characterization of cathepsin L secreted by Sf21 insect cells.
    Authors: Johnson GD, Jiang W
    Arch. Biochem. Biophys., 2005;444(1):7-14.
    Species: Insect
    Sample Types: Cell Culture Supernates
    Applications: Immunoprecipitation

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Reviews for Insect Cathepsin L Antibody

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Insect Cathepsin L Antibody
By Anonymous on 06/28/2016
Application: NULL Sample Tested: DLD clone 2C2 human colon adenocarcinoma cell line Species: Insect