Insect Cathepsin L Antibody

Name: Insect Cathepsin L Antibody
Brand: R&D Systems
SKU: MAB22591-SP
Rating: 5 (1 reviews)

Catalog #: MAB22591
9 Citations 1 Review Datasheet / COA / SDS

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MAB22591
MAB22591-SP

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Product Details

Citations (9)

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Supplemental Products

Reviews (1)

Summary Preparation and Storage Reconstitution Calculator Background Product Datasheets Related Research Areas

Insect Cathepsin L Antibody Summary

Species Reactivity

Insect

Specificity

Detects pro and mature forms of Insect Cathepsin L in direct ELISAs and Western blots. In Western blots, less than 5% cross-reactivity with recombinant human Cathepsin L is observed and no cross-reactivity with recombinant mouse Cathepsin L is observed.

Source

Monoclonal Mouse IgG₁ Clone # 193702

Purification

Protein A or G purified from hybridoma culture supernatant

Immunogen

S. frugiperda ovarian cell line Sf 21-derived Insect Cathepsin L

Formulation

Lyophilized from a 0.2 μm filtered solution in PBS with Trehalose. *Small pack size (SP) is supplied either lyophilized or as a 0.2 µm filtered solution in PBS.

Applications

Recommended Concentration

Sample

Western Blot

1 µg/mL

Recombinant Insect Cathepsin L

Immunoprecipitation

25 µg/mL

Conditioned cell culture medium spiked with Recombinant Insect Cathepsin L, see our available Western blot detection antibodies

Please Note: Optimal dilutions should be determined by each laboratory for each application. General Protocols are available in the Technical Information section on our website.

Reconstitution Calculator

Preparation and Storage

Reconstitution

Reconstitute at 0.5 mg/mL in sterile PBS.

Reconstitution Buffer Available

Reconstitution Buffer 1 (PBS)

Shipping

The product is shipped at ambient temperature. Upon receipt, store it immediately at the temperature recommended below. *Small pack size (SP) is shipped with polar packs. Upon receipt, store it immediately at -20 to -70 °C

Stability & Storage

Use a manual defrost freezer and avoid repeated freeze-thaw cycles.

12 months from date of receipt, -20 to -70 °C as supplied.
1 month, 2 to 8 °C under sterile conditions after reconstitution.
6 months, -20 to -70 °C under sterile conditions after reconstitution.

Background: Cathepsin L

The pro form of insect cell Cathepsin L is stable at neutral pH, but autoactivates in acidic buffers under reducing conditions. The activated enzyme is similar in substrate specificity to mammalian Cathepsin L, and is very active as a protease, as judged by its ability to degrade azocasein. Cystatins A, B, C, E/M, F, SA, and SN are potent inhibitors of the insect cell Cathepsin L. Like other cysteine proteases related to papain, insect cell Cathepsin L is also inhibited by E-64 and reagents which react with thiols.

Entrez Gene IDs

1514 (Human); 13039 (Mouse); 118276442 (Insect)

Alternate Names

Cathepsin L; cathepsin L1; CATL; CTSL; CTSL1; CTSLEC 3.4.22.15; EC 3.4.22; FLJ31037; Major excreted protein; MEP

Product Datasheets

Product Datasheet

COA

SDS

Related Research Areas

Citations for Insect Cathepsin L Antibody

R&D Systems personnel manually curate a database that contains references using R&D Systems products. The data collected includes not only links to publications in PubMed, but also provides information about sample types, species, and experimental conditions.

9 Citations: Showing 1 - 9
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Neuronal activity induces glucosylceramide that is secreted via exosomes for lysosomal degradation in glia
Authors: L Wang, G Lin, Z Zuo, Y Li, SK Byeon, A Pandey, HJ Bellen
Oncogene, 2022;8(28):eabn3326.
Species: Drosophila melanogaster
Sample Types: Whole Tissue
Applications: IHC
Phenylbutyrate modulates polyamine acetylase and ameliorates Snyder-Robinson syndrome in a Drosophila model and patient cells
Authors: X Tao, Y Zhu, Z Diaz-Perez, SH Yu, JR Foley, TM Stewart, RA Casero, R Steet, RG Zhai
JCI Insight, 2022;7(13):.
Species: Fly
Sample Types: Tissue Homogenates
Applications: Western Blot
Integrated analysis of the aging brain transcriptome and proteome in tauopathy
Authors: CG Mangleburg, T Wu, HK Yalamanchi, C Guo, YC Hsieh, DM Duong, EB Dammer, PL De Jager, NT Seyfried, Z Liu, JM Shulman
Mol Neurodegener, 2020;15(1):56.
Species: Drosophila
Sample Types: Tissue Homogenates
Applications: Western Blot
VAMP associated proteins are required for autophagic and lysosomal degradation by promoting a PtdIns4P-mediated endosomal pathway
Authors: D Mao, G Lin, B Tepe, Z Zuo, KL Tan, M Senturk, S Zhang, BR Arenkiel, M Sardiello, HJ Bellen
Autophagy, 2019;0(0):.
Species: Human
Sample Types: Whole Tissue
Applications: IHC
Lysosomal Machinery Drives Extracellular Acidification to Direct Non-apoptotic Cell Death
Authors: AA Mondragon, A Yalonetska, AJ Ortega, Y Zhang, O Naranjo, J Elguero, WS Chung, K McCall
Cell Rep, 2019;27(1):11-19.e3.
Species: Insect - Drosophila
Sample Types: Whole Tissue
Applications: IHC
Ubiquilins regulate autophagic flux through mTOR signalling and lysosomal acidification
Authors: M ?entürk, G Lin, Z Zuo, D Mao, E Watson, AG Mikos, HJ Bellen
Nat. Cell Biol., 2019;21(3):384-396.
Species: Insect
Sample Types: Cell Lysates
Applications: Western Blot
Spermine synthase deficiency causes lysosomal dysfunction and oxidative stress in models of Snyder-Robinson syndrome
Authors: C Li, JM Brazill, S Liu, C Bello, Y Zhu, M Morimoto, L Cascio, R Pauly, Z Diaz-Perez, MCV Malicdan, H Wang, L Boccuto, CE Schwartz, WA Gahl, CF Boerkoel, RG Zhai
Nat Commun, 2017;8(1):1257.
Species: Drosophila
Sample Types: Tissue Homogenates
Applications: Western Blot
The unique GGA clathrin adaptor of Drosophila melanogaster is not essential.
Authors: Luan S, Ilvarsonn A, Eissenberg J
PLoS ONE, 2012;7(9):e45163.
Species: Drosophila
Sample Types: Tissue Homogenates
Applications: Western Blot
Characterization of cathepsin L secreted by Sf21 insect cells.
Authors: Johnson GD, Jiang W
Arch. Biochem. Biophys., 2005;444(1):7-14.
Species: Insect
Sample Types: Cell Culture Supernates
Applications: Immunoprecipitation