Human Insulin ELISA Kit (Colorimetric)

Novus Biologicals | Catalog # NBP2-60077

Novus Biologicals
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Key Product Details

Sample Type & Volume Required Per Well

Serum, plasma, cell culture supernates and tissue homogenates (50 - 100 uL)

Sensitivity

2 uIU/mL

Assay Range

10 uIU/mL - 160 uIU/mL
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Product Specifications

Assay Type

Sandwich ELISA

Kit Type

ELISA Kit (Colorimetric)

Species

Human

Specificity

This assay has high sensitivity and excellent specificity for detection of human INS. No significant cross-reactivity or interference between human INS and analogues was observed.

Description

Assay Length: 1-5 hours

Precision

Intra-Assay Precision (Precision within an assay) %CV 15

Inter-Assay Precision (Precision between assays) %CV 15

Scientific Data Images for Human Insulin ELISA Kit (Colorimetric)

Human Insulin ELISA Kit (Colorimetric)

Human Insulin ELISA Kit (Colorimetric) [NBP2-60077] -

Human Insulin ELISA Kit (Colorimetric) [NBP2-60077] - Standard Curve Reference

Kit Contents for Human Insulin ELISA Kit (Colorimetric)

  • Adhesive Strip (For 96 wells)
  • Assay plate
  • HRP-conjugate
  • Instruction manual
  • Standard
  • Stop Solution
  • Substrate A
  • Substrate B
  • Wash Buffer (20 x concentrate)

Preparation and Storage

Shipping

The product is shipped with polar packs. Upon receipt, store it immediately at the temperature recommended below.

Stability & Storage

Storage of components varies. See protocol for specific instructions.

Background: Insulin

This assay employs the quantitative sandwich enzyme immunoassay technique. Antibody specific for INS has been pre-coated onto a microplate. Standards and samples are pipetted into the wells with a Horseradish Peroxidase (HRP) conjugated antibody specific for INS. Following a wash to remove any unbound reagent, a substrate solution is added to the wells and color develops in proportion to the amount of INS bound in the initial step. The color development is stopped and the intensity of the color is measured.

Alternate Names

IDDM2, ILPR, INS, IRDN, MODY10

Gene Symbol

INS

Additional Insulin Products

Product Documents for Human Insulin ELISA Kit (Colorimetric)

Certificate of Analysis

To download a Certificate of Analysis, please enter a lot or batch number in the search box below.

Product Specific Notices for Human Insulin ELISA Kit (Colorimetric)

This product is for research use only and is not approved for use in humans or in clinical diagnosis. ELISA Kits are guaranteed for 6 months from date of receipt.

Citations for Human Insulin ELISA Kit (Colorimetric)

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FAQs for Human Insulin ELISA Kit (Colorimetric)

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    • Q: Will you please recommend me antibodies to insulin, the most suitable for immunohistochemistry in the pancreas of guinea pigs? Desirable to be conjugated with some pigment and there was no need for secondary antibodies.
      A: I have carried out a search and the best antibody I recommend is NB600-1488. This is a primary unconjugated antibody however and you will need a secondary. If you click on the product link and then click on related products, a list of all recommended secondary antibodies will appear.
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