Rat Insulin ELISA Kit (Colorimetric)

Novus Biologicals | Catalog # NBP2-62854

Novus Biologicals
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Key Product Details

Sample Type & Volume Required Per Well

Cell culture supernatant and tissue homogenate (100 uL)

Sensitivity

0.19 ng/mL

Assay Range

0.31 - 20 ng/mL
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Product Specifications

Assay Type

Sandwich-ELISA

Kit Type

ELISA Kit (Colorimetric)

Species

Rat

Specificity

This kit recognizes Rat INS in samples. No significant cross-reactivity or interference between Rat INS and analogues was observed.

Precision

Intra-Assay Precision (Precision within an assay) %CV 5

Inter-Assay Precision (Precision between assays) %CV 5.91

Recovery for Rat Insulin ELISA Kit (Colorimetric)

Recovery

86 - 105%

Linearity

Rat Insulin ELISA Kit (Colorimetric)

Scientific Data Images for Rat Insulin ELISA Kit (Colorimetric)

Rat Insulin ELISA Kit (Colorimetric)

ELISA: Rat Insulin ELISA Kit (Colorimetric) [NBP2-62854] -

ELISA: Rat Insulin ELISA Kit (Colorimetric) [NBP2-62854] - Standard Curve Reference

Kit Contents for Rat Insulin ELISA Kit (Colorimetric)

  • Biotinylated Detection Ab Diluent
  • Concentrated Biotinylated Detection Ab (100x)
  • Concentrated HRP Conjugate (100x)
  • Concentrated Wash Buffer (25x)
  • HRP Conjugate Diluent
  • Micro ELISA Plate (Dismountable)
  • Plate Sealer
  • Product Manual
  • Reference Standard
  • Sample Diluent
  • Stop Solution
  • Substrate Reagent

Preparation and Storage

Shipping

The product is shipped with polar packs. Upon receipt, store it immediately at the temperature recommended below.

Stability & Storage

Storage of components varies. See protocol for specific instructions.

Background: Insulin

After removal of the precursor signal peptide, proinsulin is post-translationally cleaved into two chains (peptide A and peptide B) that are covalently linked via two disulfide bonds. Binding of this mature form of insulin to the insulin receptor (INSR) stimulates glucose uptake. A variety of mutant alleles with changes in the coding region have been identified. There is a read-through gene, INS-IGF2, which overlaps with this gene at the 5' region and with the IGF2 gene at the 3' region. (provided by RefSeq)

Alternate Names

IDDM2, ILPR, INS, IRDN, MODY10

Gene Symbol

INS

Additional Insulin Products

Product Documents for Rat Insulin ELISA Kit (Colorimetric)

Certificate of Analysis

To download a Certificate of Analysis, please enter a lot or batch number in the search box below.

Product Specific Notices for Rat Insulin ELISA Kit (Colorimetric)

This product is for research use only and is not approved for use in humans or in clinical diagnosis. ELISA Kits are guaranteed for 6 months from date of receipt.

Citations for Rat Insulin ELISA Kit (Colorimetric)

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FAQs for Rat Insulin ELISA Kit (Colorimetric)

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    • Q: Will you please recommend me antibodies to insulin, the most suitable for immunohistochemistry in the pancreas of guinea pigs? Desirable to be conjugated with some pigment and there was no need for secondary antibodies.
      A: I have carried out a search and the best antibody I recommend is NB600-1488. This is a primary unconjugated antibody however and you will need a secondary. If you click on the product link and then click on related products, a list of all recommended secondary antibodies will appear.
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