Insulin R/CD220 Antibody (83-7) - Chimeric - Azide and BSA Free

Novus Biologicals | Catalog # NBP2-81039

Recombinant Monoclonal Antibody
Novus Biologicals
100% Guarantee

Key Product Details

Species Reactivity

Human

Applications

Immunohistochemistry, Immunohistochemistry-Paraffin, ELISA, Flow Cytometry, Immunoprecipitation

Label

Unconjugated

Antibody Source

Recombinant Monoclonal Rabbit IgG Kappa Clone # 83-7

Format

Azide and BSA Free
View all Insulin R/CD220 Primary Antibodies »

Product Specifications

Immunogen

Epitope within amino acids 140-301 (the cysteine rich region) of the extracellular domain of the human Insulin Receptor alpha.

Specificity

Recognises the extracellular domain of the Insulin R/CD220. Amino acids 469 - 592 (exon 7 and 8) of Insulin R/CD220.

Clonality

Monoclonal

Host

Rabbit

Isotype

IgG Kappa

Scientific Data Images for Insulin R/CD220 Antibody (83-7) - Chimeric - Azide and BSA Free

Flow Cytometry: Insulin R/CD220 Antibody (83-7) - Chimeric - Azide and BSA Free [NBP2-81039]

Flow Cytometry: Insulin R/CD220 Antibody (83-7) - Chimeric - Azide and BSA Free [NBP2-81039]

Flow Cytometry: Insulin R/CD220 Antibody (83-7) [NBP2-81039] - Flow-cytometry using the Insulin R/CD220 Antibody (83-7) [NBP2-81039]. Jurkat cells were stained with unimmunized rabbit IgG antibody (black line) or the rabbit-chimeric version of 83-7 (NBP2-81039, blue line) at a concentration of 10 ug/ml for 30 mins at RT. After washing, bound antibody was detected using anti-rabbit IgG JK (FITC-conjugate) antibody at 2 ug/ml and cells analyzed on a FACSCanto flow-cytometer.

Applications for Insulin R/CD220 Antibody (83-7) - Chimeric - Azide and BSA Free

Application
Recommended Usage

ELISA

Optimal dilutions of this antibody should be experimentally determined.

Flow Cytometry

Optimal dilutions of this antibody should be experimentally determined.

Immunohistochemistry

Optimal dilutions of this antibody should be experimentally determined.

Immunohistochemistry-Paraffin

Optimal dilutions of this antibody should be experimentally determined.

Immunoprecipitation

Optimal dilutions of this antibody should be experimentally determined.
Application Notes
This chimeric rabbit antibody was made using the variable domain sequences of the original murine IgG1 format, for improved compatibility with existing reagents, assays and techniques.

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Flow Cytometry

Formulation, Preparation, and Storage

Purification

Protein A purified

Formulation

PBS

Format

Azide and BSA Free

Preservative

0.02% Proclin 300

Concentration

1 mg/ml

Shipping

The product is shipped with polar packs. Upon receipt, store it immediately at the temperature recommended below.

Stability & Storage

Store at 4C for up to 3 months. For longer storage, aliquot and store at -20C.

Background: Insulin R/CD220

Insulin receptor plays a major part in the regulation of glucose homeostasis, which when malfunctioning can result in diabetes and cancer. Encoded by the INSR gene, insulin receptor has two isoforms produced by alternative splicing, HIR-B and HIR-A.

Long Name

Insulin Receptor

Alternate Names

CD220, INSR, InsulinR

Gene Symbol

INSR

Additional Insulin R/CD220 Products

Product Documents for Insulin R/CD220 Antibody (83-7) - Chimeric - Azide and BSA Free

Certificate of Analysis

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Product Specific Notices for Insulin R/CD220 Antibody (83-7) - Chimeric - Azide and BSA Free

This product is for research use only and is not approved for use in humans or in clinical diagnosis. Primary Antibodies are guaranteed for 1 year from date of receipt.

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Protocols

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FAQs

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