Integrin alpha 2b/CD41 Antibody - BSA Free

Novus Biologicals | Catalog # NBP1-84581

Novus Biologicals
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Key Product Details

Validated by

Orthogonal Validation, Independent Antibodies

Species Reactivity

Human

Applications

Validated:

Immunohistochemistry, Immunohistochemistry-Paraffin, Simple Western

Cited:

Western Blot

Label

Unconjugated

Antibody Source

Polyclonal Rabbit IgG

Format

BSA Free
View all Integrin alpha 2b/CD41 Primary Antibodies »

Product Specifications

Immunogen

This antibody was developed against Recombinant Protein corresponding to amino acids: GATGHNIPQKLSLNAELQLDRQKPRQGRRVLLLGSQQAGTTLNLDLGGKHSPICHTTMAFLRDEADFRDKLSPIVLSLNVSLPPTEAGMAPAV

Reactivity Notes

Immunogen displays the following percentage of sequence identity for non-tested species: Mouse (85%), Rat (82%)

Clonality

Polyclonal

Host

Rabbit

Isotype

IgG

Scientific Data Images for Integrin alpha 2b/CD41 Antibody - BSA Free

Simple Western: Integrin alpha 2b/CD41 Antibody [NBP1-84581]

Simple Western: Integrin alpha 2b/CD41 Antibody [NBP1-84581]

Simple Western: Integrin alpha 2b/CD41 Antibody [NBP1-84581] - Simple Western lane view shows a specific band for Integrin alpha 2b/CD41 in 0.2 mg/ml of HEL lysate(s). This experiment was performed under reducing conditions using the 12-230 kDa separation systems.
Simple Western: Integrin alpha 2b/CD41 Antibody [NBP1-84581]

Simple Western: Integrin alpha 2b/CD41 Antibody [NBP1-84581]

Simple Western: Integrin alpha 2b/CD41 Antibody [NBP1-84581] - Electropherogram image of the corresponding Simple Western lane view. Integrin alpha 2b/CD41 antibody was used at 1:25 dilution on HEL lysate(s) respectively.
Integrin alpha 2b/CD41 Antibody - BSA Free Immunohistochemistry-Paraffin: Integrin alpha 2b/CD41 Antibody - BSA Free [NBP1-84581]

Immunohistochemistry-Paraffin: Integrin alpha 2b/CD41 Antibody - BSA Free [NBP1-84581]

Staining of human kidney shows no positivity in cells in tubules as expected.
Integrin alpha 2b/CD41 Antibody - BSA Free Immunohistochemistry-Paraffin: Integrin alpha 2b/CD41 Antibody - BSA Free [NBP1-84581]

Immunohistochemistry-Paraffin: Integrin alpha 2b/CD41 Antibody - BSA Free [NBP1-84581]

Staining of human liver shows no positivity in hepatocytes as expected.
Integrin alpha 2b/CD41 Antibody - BSA Free Immunohistochemistry-Paraffin: Integrin alpha 2b/CD41 Antibody - BSA Free [NBP1-84581]

Immunohistochemistry-Paraffin: Integrin alpha 2b/CD41 Antibody - BSA Free [NBP1-84581]

Staining of human bone marrow shows strong cytoplasmic positivity in hematopoietic cells.
Integrin alpha 2b/CD41 Antibody - BSA Free Immunohistochemistry-Paraffin: Integrin alpha 2b/CD41 Antibody - BSA Free [NBP1-84581]

Immunohistochemistry-Paraffin: Integrin alpha 2b/CD41 Antibody - BSA Free [NBP1-84581]

Staining of human tonsil shows no positivity in non-germinal center cells as expected.
Integrin alpha 2b/CD41 Antibody - BSA Free Immunohistochemistry-Paraffin: Integrin alpha 2b/CD41 Antibody - BSA Free [NBP1-84581]

Immunohistochemistry-Paraffin: Integrin alpha 2b/CD41 Antibody - BSA Free [NBP1-84581]

Analysis in human bone marrow and tonsil tissues using HPA031170 antibody. Corresponding ITGA2B RNA-seq data are presented for the same tissues.
Integrin alpha 2b/CD41 Antibody - BSA Free Immunohistochemistry-Paraffin: Integrin alpha 2b/CD41 Antibody - BSA Free [NBP1-84581]

Staining of human bone marrow, kidney, liver and tonsil using Anti-ITGA2B antibody NBP1-84581 (A) shows similar protein distribution across tissues to independent antibody HPA031168 (B).

Staining of human bone marrow, kidney, liver and tonsil using Anti-ITGA2B antibody HPA031170 (A) shows similar protein distribution across tissues to independent antibody HPA031168 (B).

Applications for Integrin alpha 2b/CD41 Antibody - BSA Free

Application
Recommended Usage

Immunohistochemistry

1:2500 - 1:5000

Immunohistochemistry-Paraffin

1:2500 - 1:5000

Simple Western

1:25
Application Notes

For IHC-Paraffin, HIER pH 6 retrieval is recommended. In Simple Western only 10-15 uL of the recommended dilution is used per data point.
See Simple Western Antibody Database for Simple Western validation: Tested in HEL lysates, separated by Size, antibody dilution of 1:25

Formulation, Preparation, and Storage

Purification

Affinity purified

Formulation

PBS (pH 7.2) and 40% Glycerol

Format

BSA Free

Preservative

0.02% Sodium Azide

Concentration

Concentrations vary lot to lot. See vial label for concentration. If unlisted please contact technical services.

Shipping

The product is shipped with polar packs. Upon receipt, store it immediately at the temperature recommended below.

Stability & Storage

Store at 4C short term. Aliquot and store at -20C long term. Avoid freeze-thaw cycles.

Background: Integrin alpha 2b/CD41

Integrin alpha 2b (CD41) is a calcium-dependent, noncovalently associated heterodimer and contains a heavy chain (GPIIb alpha) and a light chain (GPIIb beta) linked by a single disulfide bond. The Integrin alpha 2B chain interacts with the Integrin beta 3 subunit/CD61 to form the platelet glycoprotein complex, gpIIb/IIIa. It is expressed on platelets and megakaryocytes. Ligands for the gpIIb/IIIa heterodimer include fibrinogen, von Willebrand factor, fibronectin, vitronectin, and thrombospondin. The gpIIb/IIIa complex is the major Integrin on platelets and is important for platelet adhesion and aggregation.

Alternate Names

CD41, GP2B, GPIIb, GTA, HPA3, ITGA2b

Gene Symbol

ITGA2B

Additional Integrin alpha 2b/CD41 Products

Product Documents for Integrin alpha 2b/CD41 Antibody - BSA Free

Certificate of Analysis

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Product Specific Notices for Integrin alpha 2b/CD41 Antibody - BSA Free

This product is for research use only and is not approved for use in humans or in clinical diagnosis. Primary Antibodies are guaranteed for 1 year from date of receipt.

Citations for Integrin alpha 2b/CD41 Antibody - BSA Free

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Protocols

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