Integrin alpha 5/CD49e Antibody (CL6940) - BSA Free

Name: Integrin alpha 5/CD49e Antibody (CL6940) - BSA Free
Brand: Novus Biologicals
SKU: NBP2-76518
Price: 429 USD
Availability: InStock

Novus Biologicals | Catalog # NBP2-76518

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Product Specifications
Scientific Data
Applications
Preparation & Storage
Background
Product Documents
Specific Notices
Research Areas

Key Product Details

Validated by

Knockout/Knockdown, Orthogonal Validation

Species Reactivity

Human

Applications

Immunohistochemistry, Immunohistochemistry-Paraffin, Western Blot, Knockdown Validated

Label

Unconjugated

Antibody Source

Monoclonal Mouse IgG₁ Clone # CL6940

Format

BSA Free

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Product Specifications

Immunogen

This antibody was developed using a recombinant protein derived from P08648, with the exact immunogen sequence remaining proprietary.

Reactivity Notes

Immunogen displays the following percentage of sequence identity for non-tested species: Mouse (85)% and Rat (82)%

Clonality

Monoclonal

Host

Mouse

Isotype

IgG₁

Scientific Data Images for Integrin alpha 5/CD49e Antibody (CL6940) - BSA Free

Western Blot: Integrin alpha 5/CD49e Antibody (CL6940) [NBP2-76518]

Western Blot: Integrin alpha 5/CD49e Antibody (CL6940) [NBP2-76518] - Analysis in U-87MG ATCC cells transfected with control siRNA, target specific siRNA probe #1 and #2, using Anti-ITGA5 antibody. Remaining relative intensity is presented. Loading control: Anti-GAPDH.

Immunohistochemistry-Paraffin: Integrin alpha 5/CD49e Antibody (CL6940) [NBP2-76518]

Immunohistochemistry-Paraffin: Integrin alpha 5/CD49e Antibody (CL6940) [NBP2-76518] - Staining of human testis shows no positivity in cells in seminiferous ducts as expected.

Western Blot: Integrin alpha 5/CD49e Antibody (CL6940) [NBP2-76518]

Western Blot: Integrin alpha 5/CD49e Antibody (CL6940) [NBP2-76518] - Analysis in human cell line U-87 MG.

Immunohistochemistry-Paraffin: Integrin alpha 5/CD49e Antibody (CL6940) [NBP2-76518]

Immunohistochemistry-Paraffin: Integrin alpha 5/CD49e Antibody (CL6940) [NBP2-76518] - Analysis in human placenta and testis tissues. Corresponding ITGA5 RNA-seq data are presented for the same tissues.

Immunohistochemistry-Paraffin: Integrin alpha 5/CD49e Antibody (CL6940) [NBP2-76518]

Immunohistochemistry-Paraffin: Integrin alpha 5/CD49e Antibody (CL6940) [NBP2-76518] - Staining of human placenta shows strong membranous positivity in trophoblastic cells.

Immunohistochemistry-Paraffin: Integrin alpha 5/CD49e Antibody (CL6940) [NBP2-76518]

Immunohistochemistry-Paraffin: Integrin alpha 5/CD49e Antibody (CL6940) [NBP2-76518] - Staining of human endometrium shows moderate membranous positivity in smooth muscle cells.

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Applications for Integrin alpha 5/CD49e Antibody (CL6940) - BSA Free

Application

Recommended Usage

Immunohistochemistry

1:5000 - 1:10000

Immunohistochemistry-Paraffin

1:5000 - 1:10000

Western Blot

1 ug/ml

Application Notes

For IHC-Paraffin, HIER pH 6 retrieval is recommended.

Formulation, Preparation, and Storage

Purification

Protein A purified

Formulation

PBS (pH 7.2) and 40% Glycerol

Format

BSA Free

Preservative

0.02% Sodium Azide

Concentration

1 mg/ml

Shipping

The product is shipped with polar packs. Upon receipt, store it immediately at the temperature recommended below.

Stability & Storage

Store at 4C short term. Aliquot and store at -20C long term. Avoid freeze-thaw cycles.

Background: Integrin alpha 5/CD49e

CD49e is a type I integral membrane glycoprotein, known as alpha5 integrin, VLA-5 alpha chain. It forms noncovalent heterodimer with integrin beta1 (CD29). CD49e contains two disulfide-linked subunits of 135 kDa and 24 kDa, and is mainly expressed on thymocytes, activated lymphocytes, endothelial cells, osteoblasts, melanoma, and some myeloid leukemia cells. CD49e/CD29 complex binds to fibronection and neural adhesion molecule L1. It mediates monocytes migration, involves in the regulation of cells survival and apoptosis, and co-stimulates T cell activation.

Alternate Names

CD49e, ITGA5, VLA-5 alpha

Gene Symbol

ITGA5

Additional Integrin alpha 5/CD49e Products

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Product Specific Notices for Integrin alpha 5/CD49e Antibody (CL6940) - BSA Free

This product is for research use only and is not approved for use in humans or in clinical diagnosis. Primary Antibodies are guaranteed for 1 year from date of receipt.