Integrin alpha 5/CD49e Antibody (JJ08-94)

Name: Integrin alpha 5/CD49e Antibody (JJ08-94)
Brand: Novus Biologicals
SKU: NBP2-67883
Price: 439 USD
Availability: InStock

Novus Biologicals | Catalog # NBP2-67883

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Product Documents

Recombinant Monoclonal Antibody

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Product Specifications
Scientific Data
Applications
Flow Cytometry
Preparation & Storage
Background
Product Documents
Specific Notices
Research Areas

Key Product Details

Species Reactivity

Human, Mouse, Rat

Applications

Immunohistochemistry, Immunohistochemistry-Paraffin, Western Blot, Flow Cytometry, Immunocytochemistry/ Immunofluorescence, Immunoprecipitation

Label

Unconjugated

Antibody Source

Recombinant Monoclonal Rabbit IgG Clone # JJ08-94 expressed in HEK293

View all Integrin alpha 5/CD49e Primary Antibodies »

Product Specifications

Immunogen

Synthetic peptide within C-terminal human Integrin alpha 5/CD49e. (SwissProt: P08648 Human; SwissProt: P11688 Mouse; Unigene:100796 Rat)

Localization

Membrane, Cell junction, Cell surface.

Clonality

Monoclonal

Host

Rabbit

Isotype

IgG

Scientific Data Images for Integrin alpha 5/CD49e Antibody (JJ08-94)

Western Blot: Integrin alpha 5/CD49e Antibody (JJ08-94) [NBP2-67883]

Western Blot: Integrin alpha 5/CD49e Antibody (JJ08-94) [NBP2-67883] - Western blot analysis of Integrin alpha 5/CD49e on NIH/3T3 cell lysates. Proteins were transferred to a PVDF membrane and blocked with 5% BSA in PBS for 1 hour at room temperature. The primary antibody (1/500) was used in 5% BSA at room temperature for 2

Immunocytochemistry/ Immunofluorescence: Integrin alpha 5/CD49e Antibody (JJ08-94) [NBP2-67883]

Immunocytochemistry/Immunofluorescence: Integrin alpha 5/CD49e Antibody (JJ08-94) [NBP2-67883] - Staining Integrin alpha 5 in Hela cells (green). The nuclear counter stain is DAPI (blue). Cells were fixed in paraformaldehyde, permeabilised with 0.25% Triton X100/PBS.

Immunohistochemistry-Paraffin: Integrin alpha 5/CD49e Antibody (JJ08-94) [NBP2-67883]

Immunohistochemistry-Paraffin: Integrin alpha 5/CD49e Antibody (JJ08-94) [NBP2-67883] - Analysis of paraffin-embedded mouse uterus tissue using anti-Integrin alpha 5 antibody. Counter stained with hematoxylin.

Flow Cytometry: Integrin alpha 5/CD49e Antibody (JJ08-94) [NBP2-67883]

Flow Cytometry: Integrin alpha 5/CD49e Antibody (JJ08-94) [NBP2-67883] - Analysis of Jurkat cells with Integrin alpha 5 antibody at 1/50 dilution (red) compared with an unlabelled control (cells without incubation with primary antibody; black). Alexa Fluor 488-conjugated goat anti rabbit IgG was used as the secondary antibody.

Immunocytochemistry/ Immunofluorescence: Integrin alpha 5/CD49e Antibody (JJ08-94) [NBP2-67883]

Immunocytochemistry/Immunofluorescence: Integrin alpha 5/CD49e Antibody (JJ08-94) [NBP2-67883] - Staining Integrin alpha 5 in A549 cells (green). The nuclear counter stain is DAPI (blue). Cells were fixed in paraformaldehyde, permeabilised with 0.25% Triton X100/PBS.

Immunohistochemistry-Paraffin: Integrin alpha 5/CD49e Antibody (JJ08-94) [NBP2-67883]

Immunohistochemistry-Paraffin: Integrin alpha 5/CD49e Antibody (JJ08-94) [NBP2-67883] - Analysis of paraffin-embedded human kidney tissue using anti-Integrin alpha 5 antibody. Counter stained with hematoxylin.

Immunohistochemistry-Paraffin: Integrin alpha 5/CD49e Antibody (JJ08-94) [NBP2-67883]

Immunohistochemistry-Paraffin: Integrin alpha 5/CD49e Antibody (JJ08-94) [NBP2-67883] - Analysis of paraffin-embedded human tonsil tissue using anti-Integrin alpha 5 antibody. Counter stained with hematoxylin.

Immunohistochemistry-Paraffin: Integrin alpha 5/CD49e Antibody (JJ08-94) [NBP2-67883]

Immunohistochemistry-Paraffin: Integrin alpha 5/CD49e Antibody (JJ08-94) [NBP2-67883] - Analysis of paraffin-embedded human uterus tissue using anti-Integrin alpha 5 antibody. Counter stained with hematoxylin.

Western Blot: Integrin alpha 5/CD49e Antibody (JJ08-94) [NBP2-67883]

Western blot analysis on different lysates with NBP2-67883 at 1/1,000 dilution. Lane 1: HeLa cell lysate Lane 2: A549 cell lysate Lane 3: U-937 cell lysate Lane 4: NIH/3T3 cell lysate Lane 5: COS-1 cell lysate Lane 6: Mouse liver tissue lysate Lane 7: Rat liver tissue lysate Lysates/proteins at 20 µg/Lane. Predicted band size: 115 kDa Observed band size: 140/19 kDa Exposure time: 1 minute 2 seconds; 4-20% SDS-PAGE gel. Proteins were transferred to a PVDF membrane and blocked with 5% NFDM/TBST for 1 hour at room temperature. NBP2-67883 at 1/1,000 dilution was used in 5% NFDM/TBST at 4℃ overnight. Goat Anti-Rabbit IgG [HRP] Secondary Antibody at 1/50,000 dilution was used for 1 hour at room temperature.

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Applications for Integrin alpha 5/CD49e Antibody (JJ08-94)

Application

Recommended Usage

Flow Cytometry

1:50-1:100

Immunocytochemistry/ Immunofluorescence

1:50-1:200

Immunohistochemistry-Paraffin

1:50-1:200

Western Blot

1:1000-1:2000

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Advanced Features

Spectra Viewer - Custom analysis of spectra from multiple fluorochromes
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Antigen Density Selector - Match fluorochrome brightness with antigen density

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Formulation, Preparation, and Storage

Purification

Protein A purified

Formulation

TBS (pH7.4), 0.05% BSA, 40% Glycerol

Preservative

0.05% Sodium Azide

Concentration

1 mg/ml

Shipping

The product is shipped with polar packs. Upon receipt, store it immediately at the temperature recommended below.

Stability & Storage

Store at 4C short term. Aliquot and store at -20C long term. Avoid freeze-thaw cycles.

Background: Integrin alpha 5/CD49e

CD49e is a type I integral membrane glycoprotein, known as alpha5 integrin, VLA-5 alpha chain. It forms noncovalent heterodimer with integrin beta1 (CD29). CD49e contains two disulfide-linked subunits of 135 kDa and 24 kDa, and is mainly expressed on thymocytes, activated lymphocytes, endothelial cells, osteoblasts, melanoma, and some myeloid leukemia cells. CD49e/CD29 complex binds to fibronection and neural adhesion molecule L1. It mediates monocytes migration, involves in the regulation of cells survival and apoptosis, and co-stimulates T cell activation.

Alternate Names

CD49e, ITGA5, VLA-5 alpha

Gene Symbol

ITGA5

Additional Integrin alpha 5/CD49e Products

Product Documents for Integrin alpha 5/CD49e Antibody (JJ08-94)

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Certificate of Analysis

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Product Specific Notices for Integrin alpha 5/CD49e Antibody (JJ08-94)

This product is for research use only and is not approved for use in humans or in clinical diagnosis. Primary Antibodies are guaranteed for 1 year from date of receipt.