Integrin alpha V/CD51 Antibody - BSA Free

Name: Integrin alpha V/CD51 Antibody - BSA Free
Brand: Novus Biologicals
SKU: NBP1-85746
Price: 549 USD
Availability: InStock

Novus Biologicals | Catalog # NBP1-85746

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Product Documents

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Product Specifications
Scientific Data
Applications
Preparation & Storage
Background
Product Documents
Specific Notices
Research Areas

Key Product Details

Species Reactivity

Validated:

Human

Predicted:

Rat (90%). Backed by our 100% Guarantee.

Applications

Validated:

Immunohistochemistry, Immunohistochemistry-Paraffin, Immunocytochemistry/ Immunofluorescence, Simple Western

Cited:

In vivo assay

Label

Unconjugated

Antibody Source

Polyclonal Rabbit IgG

Format

BSA Free

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Product Specifications

Immunogen

This antibody was developed against Recombinant Protein corresponding to amino acids: DHLITKRDLALSEGDIHTLGCGVAQCLKIVCQVGRLDRGKSAILYVKSLLWTETFMNKENQNHSYSLKSSASFNVIEFPYKNLPIEDITNSTLVTTNVTWG

Reactivity Notes

Immunogen displays the following percentage of sequence identity for non-tested species: Mouse (88%)

Clonality

Polyclonal

Host

Rabbit

Isotype

IgG

Scientific Data Images for Integrin alpha V/CD51 Antibody - BSA Free

Immunohistochemistry-Paraffin: Integrin alpha V/CD51 Antibody [NBP1-85746]

Immunohistochemistry-Paraffin: Integrin alpha V/CD51 Antibody [NBP1-85746] - Staining of human placenta shows strong cytoplasmic and membranous positivity in trophoblastic cells.

Simple Western: Integrin alpha V/CD51 Antibody [NBP1-85746]

Simple Western: Integrin alpha V/CD51 Antibody [NBP1-85746] - Simple Western lane view shows a specific band for ITGAV in 0.2 mg/ml of RT4 lysate(s). This experiment was performed under reducing conditions using the 12-230 kDa separation system.

Simple Western: Integrin alpha V/CD51 Antibody [NBP1-85746]

Simple Western: Integrin alpha V/CD51 Antibody [NBP1-85746] - Electropherogram image of the corresponding Simple Western lane view. ITGAV antibody was used at 1:25 dilution on RT4 lysate(s) respectively.

Immunocytochemistry/ Immunofluorescence: Integrin alpha V/CD51 Antibody - BSA Free [NBP1-85746]

Staining of human cell line A-431 shows localization to cytosol & focal adhesion sites.

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Applications for Integrin alpha V/CD51 Antibody - BSA Free

Application

Recommended Usage

Immunocytochemistry/ Immunofluorescence

0.25-2 ug/ml

Immunohistochemistry

1:200 - 1:500

Immunohistochemistry-Paraffin

1:200 - 1:500

Simple Western

1:25

Application Notes

For IHC-Paraffin, HIER pH 6 retrieval is recommended. ICC/IF Fixation Permeabilization: Use PFA/Triton X-100.

In Simple Western only 10 - 15 uL of the recommended dilution is used per data point.
See Simple Western Antibody Database for Simple Western validation: Tested in RT-4 lysate, separated by Size, antibody dilution of 1:25, apparent MW was 173 kDa.

Formulation, Preparation, and Storage

Purification

Affinity purified

Formulation

PBS (pH 7.2) and 40% Glycerol

Format

BSA Free

Preservative

0.02% Sodium Azide

Concentration

Concentrations vary lot to lot. See vial label for concentration. If unlisted please contact technical services.

Shipping

The product is shipped with polar packs. Upon receipt, store it immediately at the temperature recommended below.

Stability & Storage

Store at 4C short term. Aliquot and store at -20C long term. Avoid freeze-thaw cycles.

Background: Integrin alpha V/CD51

Integrin alpha V chain interacts with the integrin beta 3 subunit/CD61 to form the alpha-V-beta-3 heterodimer/vitronectin receptor. It is expressed on endothelial cells, some activated leukocytes, NK cells, macrophages, neutrophils, and platelets. Integrin alpha V also forms heterodimers with the integrin beta 1, beta 5, beta 6, and beta 8 subunits. Alpha-V-beta-3 is an activation dependent receptor for platelet attachment and spreading on vitronectin and other matrix components.

Alternate Names

CD51, ITGAV, MSK8, Vitronectin Receptor alpha, VNRA

Gene Symbol

ITGAV

Additional Integrin alpha V/CD51 Products

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Product Specific Notices for Integrin alpha V/CD51 Antibody - BSA Free

This product is for research use only and is not approved for use in humans or in clinical diagnosis. Primary Antibodies are guaranteed for 1 year from date of receipt.