Integrin beta 1/CD29 Antibody (MEM-101A) - BSA Free

Novus Biologicals | Catalog # NB500-383

Clone MEM-101A was used by HLDA to establish CD designation.
Novus Biologicals

Key Product Details

Species Reactivity

Validated:

Human, Porcine, Canine, Mouse (Negative)

Cited:

Porcine, Canine

Applications

Validated:

Western Blot, Flow Cytometry, Immunoprecipitation, CyTOF-ready

Cited:

Flow Cytometry, Immunocytochemistry/ Immunofluorescence

Label

Unconjugated

Antibody Source

Monoclonal Mouse IgG1 Clone # MEM-101A

Format

BSA Free
View all Integrin beta 1/CD29 Primary Antibodies »

Product Specifications

Immunogen

Raji Burkitt's lymphoma cell line (NM_002211.3).

Marker

Stem Cell Marker

Specificity

The antibody MEM-101A reacts with CD29 (integrin beta1 chain), a 130 kDa single chain type I glycoprotein expressed as a heterodimer (non-covalently associated with the integrin alpha subunits 1-6). CD29 is broadly expressed on majority of hematopoietic and non-hematopoietic cells (leukocytes, platelets, fibroblasts, endothelial cells, epithelial cells and mast cells). HLDA VI; WS Code AS A048

Clonality

Monoclonal

Host

Mouse

Isotype

IgG1

Theoretical MW

130 kDa.
Disclaimer note: The observed molecular weight of the protein may vary from the listed predicted molecular weight due to post translational modifications, post translation cleavages, relative charges, and other experimental factors.

Description

Novus Biologicals Mouse Integrin beta 1/CD29 Antibody (MEM-101A) - BSA Free (NB500-383) is a monoclonal antibody validated for use in WB, Flow, ICC/IF and IP. Anti-Integrin beta 1/CD29 Antibody: Cited in 7 publications. All Novus Biologicals antibodies are covered by our 100% guarantee.

Scientific Data Images for Integrin beta 1/CD29 Antibody (MEM-101A) - BSA Free

Western Blot: Integrin beta 1/CD29 Antibody (MEM-101A)BSA Free [NB500-383]

Western Blot: Integrin beta 1/CD29 Antibody (MEM-101A)BSA Free [NB500-383]

Western Blot: Integrin beta 1/CD29 Antibody (MEM-101A) [NB500-383] - Western Blotting analysis (non-reducing conditions) of isolated peripheral blood lymphocytes of various species using anti-Integrin beta 1 Antibody (MEM-101A). Lane 1: lysate of human PBL Lane 2: lysate of canine PBL Lane 3: lysate of porcine PBL.
Flow Cytometry: Integrin beta 1/CD29 Antibody (MEM-101A) - BSA Free [NB500-383]

Flow Cytometry: Integrin beta 1/CD29 Antibody (MEM-101A) - BSA Free [NB500-383]

Flow Cytometry: Integrin beta 1/CD29 Antibody (MEM-101A) [NB500-383] - Staining of human peripheral blood with anti-human CD29 (MEM-101A) PE.
Flow Cytometry: Integrin beta 1/CD29 Antibody (MEM-101A) - BSA Free [NB500-383]

Flow Cytometry: Integrin beta 1/CD29 Antibody (MEM-101A) - BSA Free [NB500-383]

Flow Cytometry: Integrin beta 1/CD29 Antibody (MEM-101A) [NB500-383] - Surface staining of human peripheral blood with anti-human CD29 (MEM-101A) APC conjugated.

Applications for Integrin beta 1/CD29 Antibody (MEM-101A) - BSA Free

Application
Recommended Usage

Flow Cytometry

2 ug/ml

Immunoprecipitation

1:50

Western Blot

2 ug/ml
Application Notes
Purity is > 95% (by SDS-PAGE). Western Blot - Sample preparation: buffer with laurylmaltoside, 2 x non-reducing SDS Application note: Non-reducing conditions. SDS-PAGE (6% separating gel, 4% stacking gel). Recommended secondary antibody - anti mouse IgG/HRP, dilution 1:2000, 60 min on vertical incubator. This antibody is CyTOF ready.

Flow Cytometry Panel Builder

Bio-Techne Knows Flow Cytometry

Save time and reduce costly mistakes by quickly finding compatible reagents using the Panel Builder Tool.

Advanced Features

  • Spectra Viewer - Custom analysis of spectra from multiple fluorochromes
  • Spillover Popups - Visualize the spectra of individual fluorochromes
  • Antigen Density Selector - Match fluorochrome brightness with antigen density
Build Your Panel Now
Flow Cytometry

Formulation, Preparation, and Storage

Purification

Protein A purified

Formulation

Phosphate buffered saline (PBS), pH 7.4

Format

BSA Free

Preservative

15mM Sodium Azide

Concentration

1 mg/ml

Shipping

The product is shipped with polar packs. Upon receipt, store it immediately at the temperature recommended below.

Stability & Storage

Store at 4C. Do not freeze.

Background: Integrin beta 1/CD29

CD29 (beta1 integrin subunit, GPIIa) forms non-covalently linked heterodimers with at least 6 different alpha chains (alpha1-alpha6, CD49a-f) determining the binding properties of beta1 (VLA) integrins. These integrins mediate cell adhesion to collagen, fibronectin, laminin and other extracellular matrix (ECM) components. This interaction hinders cell death, whereas disruption of anchorage to ECM leads to apoptosis. Decreased expression of most beta1 integrins correlates with acquiring multidrug resistance of tumour cells during selection in presence of antitumour drug. In platelets, translocation of intracellular pool of beta1 integrins to the plasma membrane following thrombin stimulation. These integrins are also up-regulated in leukocytes during emigration and extravascular migration and appear to be critically involved in regulating the immune cell trafficking from blood to tissue, as well as in regulating tissue damage and disease symptoms related to inflammatory bowel disease. Through a beta1 integrin-dependent mechanism, fibronectin and type I collagen enhance cytokine secretion of human airway smooth muscle in response to IL-1beta.

Alternate Names

CD29, ITGB1

Gene Symbol

ITGB1

Additional Integrin beta 1/CD29 Products

Product Documents for Integrin beta 1/CD29 Antibody (MEM-101A) - BSA Free

Certificate of Analysis

To download a Certificate of Analysis, please enter a lot or batch number in the search box below.

Download SDS

Product Specific Notices for Integrin beta 1/CD29 Antibody (MEM-101A) - BSA Free

This product is for research use only and is not approved for use in humans or in clinical diagnosis. Primary Antibodies are guaranteed for 1 year from date of receipt.

Citations for Integrin beta 1/CD29 Antibody (MEM-101A) - BSA Free

Customer Reviews for Integrin beta 1/CD29 Antibody (MEM-101A) - BSA Free

There are currently no reviews for this product. Be the first to review Integrin beta 1/CD29 Antibody (MEM-101A) - BSA Free and earn rewards!

Have you used Integrin beta 1/CD29 Antibody (MEM-101A) - BSA Free?

Submit a review and receive an Amazon gift card!

$25/€18/£15/$25CAN/¥2500 Yen for a review with an image

$10/€7/£6/$10CAN/¥1110 Yen for a review without an image

Submit a review
Amazon Gift Card

Protocols

Find general support by application which include: protocols, troubleshooting, illustrated assays, videos and webinars.

FAQs for Integrin beta 1/CD29 Antibody (MEM-101A) - BSA Free

Showing  1 - 1 of 1 FAQ Showing All

    • Q: I'm looking for a good antibody for integrins that works in rats. I would need it for Western Blot, Immunocyto- as well as Immunohistochemistry. I'm especially interested in beta1.
      A: I would recommend NB110-57123 as it has been validated using rat samples in WB, IHC-P, and FACS. Although we do not list ICC on the datasheet for this antibody I believe it will work in ICC because it works in Flow Cytometry and has been validated to stain tissue. We have several other Integrin targets. To search for what you are looking for I recommend typing Integrin into the search area at the top of our website, then review the list of suggested targets that poplulate below the search. Once you click on a particular Integrin you can narrow the results with our filter. I recommend filtering to primary antibodies, by target name, species of interest you can filter to rat. If you so choose you can also filter further by application, host, clonality, etc.
Showing  1 - 1 of 1 FAQ Showing All
View all FAQs for Antibodies