Integrin beta 2/CD18 Antibody (60.3) - Chimeric - Azide and BSA Free

Novus Biologicals | Catalog # NBP3-12031

Recombinant Monoclonal Antibody
Novus Biologicals
100% Guarantee

Key Product Details

Species Reactivity

Human

Applications

Western Blot, ELISA, Block/Neutralize, Immunocytochemistry/ Immunofluorescence, Functional (Inhibition)

Label

Unconjugated

Antibody Source

Recombinant Monoclonal Rabbit IgG Kappa Clone # 60.3

Format

Azide and BSA Free
View all Integrin beta 2/CD18 Primary Antibodies »

Product Specifications

Immunogen

The original antibody was generated by immunizing BALB/c mice with alloantigen activated T cells.

Specificity

This antibody binds the Integrin beta 2/CD18 component of the beta-2 integrin family which is expressed by leukocytes and neutrophils.

Clonality

Monoclonal

Host

Rabbit

Isotype

IgG Kappa

Scientific Data Images for Integrin beta 2/CD18 Antibody (60.3) - Chimeric - Azide and BSA Free

Immunocytochemistry/ Immunofluorescence: Integrin beta 2/CD18 Antibody (60.3) - Chimeric - Azide and BSA Free [NBP3-12031]

Immunocytochemistry/ Immunofluorescence: Integrin beta 2/CD18 Antibody (60.3) - Chimeric - Azide and BSA Free [NBP3-12031]

Immunocytochemistry/Immunofluorescence: Integrin beta 2/CD18 Antibody (60.3) - Chimeric [NBP3-12031] - Immunofluorescence analysis of paraformaldehyde fixed Jurkat cells on Shi-fix(TM) coverslips stained with the chimeric rabbit IgG version of 60.3 (NBP3-12031) at 10 ug/ml for 1h followed by Alexa Fluor(R) 488 secondary antibody (2 ug/ml), showing membrane staining. The nuclear stain is DAPI (blue). Panels show from left-right, top-bottom NBP3-12031, DAPI, merged channels and an isotype control. The isotype control was an unknown specificity antibody followed by staining with Alexa Fluor(R) 488 secondary antibody.

Applications for Integrin beta 2/CD18 Antibody (60.3) - Chimeric - Azide and BSA Free

Application
Recommended Usage

Block/Neutralize

Optimal dilutions of this antibody should be experimentally determined.

ELISA

Optimal dilutions of this antibody should be experimentally determined.

Functional (Inhibition)

Optimal dilutions of this antibody should be experimentally determined.

Immunocytochemistry/ Immunofluorescence

Optimal dilutions of this antibody should be experimentally determined.

Western Blot

Optimal dilutions of this antibody should be experimentally determined.
Application Notes
This chimeric rabbit antibody was made using the variable domain sequences of the original Mouse IgG2a format, for improved compatibility with existing reagents, assays and techniques.

Formulation, Preparation, and Storage

Purification

Protein A purified

Formulation

PBS

Format

Azide and BSA Free

Preservative

0.02% Proclin 300

Concentration

1 mg/ml

Shipping

The product is shipped with polar packs. Upon receipt, store it immediately at the temperature recommended below.

Stability & Storage

Store at 4C for up to 3 months. For longer storage, aliquot and store at -20C.

Background: Integrin beta 2/CD18

CD18 is an integrin. Integrins are integral cell surface proteins composed of an alpha chain and a beta chain. A given chain may combine with multiple partners resulting in different integrins. For example, beta 2 combines with the alpha L chain to form the integrin LFA1, and combines with the alpha M chain to form the integrin Mac1. Integrins are known to participate in cell adhesion as well as cell surface mediated signalling. CD18 is expressed by most leucocytes. Defects in the CD18 gene result in leukocyte adhesion deficiency.

Alternate Names

CD18, ITGB2, LCAMB, LFA-1, LFA-1 beta, MAC-1 beta, MF17, p150,95 beta

Gene Symbol

ITGB2

Additional Integrin beta 2/CD18 Products

Product Documents for Integrin beta 2/CD18 Antibody (60.3) - Chimeric - Azide and BSA Free

Certificate of Analysis

To download a Certificate of Analysis, please enter a lot or batch number in the search box below.

Product Specific Notices for Integrin beta 2/CD18 Antibody (60.3) - Chimeric - Azide and BSA Free

This product is for research use only and is not approved for use in humans or in clinical diagnosis. Primary Antibodies are guaranteed for 1 year from date of receipt.

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Protocols

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FAQs

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