IRAK4 Antibody (10H4) - BSA Free

Novus Biologicals | Catalog # NBP3-15153

Recombinant Monoclonal Antibody
Novus Biologicals
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Key Product Details

Species Reactivity

Human

Applications

Western Blot, ELISA, Flow Cytometry

Label

Unconjugated

Antibody Source

Recombinant Monoclonal Rabbit IgG Clone # 10H4 expressed in HEK293

Format

BSA Free
View all IRAK4 Primary Antibodies »

Product Specifications

Immunogen

A synthesized peptide derived from human IRAK4. UniProt ID (Q9NWZ3).

Clonality

Monoclonal

Host

Rabbit

Isotype

IgG

Scientific Data Images for IRAK4 Antibody (10H4) - BSA Free

Western Blot: IRAK4 Antibody (10H4) [NBP3-15153]

Western Blot: IRAK4 Antibody (10H4) [NBP3-15153]

Western Blot: IRAK4 Antibody (10H4) [NBP3-15153] - Positive WB detected in: THP-1 whole cell lysate, U251 whole cell lysate. All lanes: IRAK4 antibody at 1:2000. Secondary: Goat polyclonal to rabbit IgG at 1/50000 dilution. Predicted band size: 52, 38 KDa. Observed band size: 55 KDa.
Flow Cytometry: IRAK4 Antibody (10H4) [NBP3-15153]

Flow Cytometry: IRAK4 Antibody (10H4) [NBP3-15153]

Flow Cytometry: IRAK4 Antibody (10H4) [NBP3-15153] - Overlay histogram showing Jurkat cells stained with IRAK4 antibody (red line) at 1:50. The cells were fixed with 70% Ethylalcohol (18h) and then incubated in 10% normal goat serum to block non-specific protein-protein interactions followedby the antibody (1ug/1*106cells) for 1 h at 4C.The secondary antibody used was FITC-conjugated goat anti-rabbit IgG (H+L) at 1/200 dilution for 30min at 4C. Control antibody (green line) was Rabbit IgG (1ug/1*106cells) used under the same conditions. Acquisition of >10,000 events was performed.

Applications for IRAK4 Antibody (10H4) - BSA Free

Application
Recommended Usage

Western Blot

1:500-1:5000

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Flow Cytometry

Formulation, Preparation, and Storage

Purification

Affinity purified

Formulation

PBS, pH 7.4, 150mM NaCl, and 50% glycerol

Format

BSA Free

Preservative

0.02% Sodium Azide

Concentration

Please see the vial label for concentration. If unlisted please contact technical services.

Shipping

The product is shipped with polar packs. Upon receipt, store it immediately at the temperature recommended below.

Stability & Storage

Store at -20 to -70C. Avoid freeze-thaw cycles.

Background: IRAK4

Interleukin-1 receptor (see IL1R; MIM 147810)-associated kinases (e.g., IRAK1; MIM 300283) are important mediators in the signal transduction of Toll-like receptor (TLR, e.g., TLR4; MIM 603030) and IL1R family members, collectively referred to as TIRs. IRAK4 functions in this signal transduction pathway.[supplied by OMIM]

Long Name

Interleukin-1 Receptor-Associated Kinase 4

Alternate Names

REN64

Gene Symbol

IRAK4

Additional IRAK4 Products

Product Documents for IRAK4 Antibody (10H4) - BSA Free

Certificate of Analysis

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Product Specific Notices for IRAK4 Antibody (10H4) - BSA Free

This product is for research use only and is not approved for use in humans or in clinical diagnosis. Primary Antibodies are guaranteed for 1 year from date of receipt.

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Protocols

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FAQs

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