Q: I recently purchased some IRAK4 recombinant protein. I was wondering if you could tell me whether or not this protein is phosphorylated.

A: This product is distributed by Novus for a Taiwanese company called Abnova. We do not perform any in-house testing or development of their products. This protein is produced in a cell-free wheat germ system with an N-terminal 26kDa GST-tag. Due to this expression system, in our experience, the proteins do not always undergo the same folding and post-translational modifications they might undergo in an endogenous cell. Abnova does not evaluate the phosphorylation status of their proteins.

Recombinant Human IRAK4 GST (N-Term) Protein

Novus Biologicals | Catalog # H00051135-P01

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Product Documents

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Product Specifications
Scientific Data
Preparation & Storage
Background
Product Documents
Specific Notices
Research Areas

Key Product Details

Source

Wheat germ

Tag

GST (N-Term)

Applications

Western Blot, ELISA, Affinity Purification, Enzyme Activity, Microarray

View all IRAK4 Proteins and Enzymes »

Product Specifications

Description

A recombinant protein with GST tag at N-terminal corresponding to the amino acids 1-460 of Human IRAK4

Source: Wheat Germ (in vitro)

Amino Acid Sequence: MNKPITPSTYVRCLNVGLIRKLSDFIDPQEGWKKLAVAIKKPSGDDRYNQFHIRRFEALLQTGKSPTSELLFDWGTTNCTVGDLVDLLIQNEFFAPASLLLPDAVPKTANTLPSKEAITVQQKQMPFCDKDRTLMTPVQNLEQSYMPPDSSSPENKSLEVSDTRFHSFSFYELKNVTNNFDERPISVGGNKMGEGGFGVVYKGYVNNTTVAVKKPAAMVDITTEELKQQFDQEIKVMAKCQHENLVELLGFSSDGDDLCLVYVYMPNGSLLDRLSCLDGTPPLSWHMRCKIAQGAANGINFLHENHHIHRDIKSANILLDEAFTAKISDFGLARASEKFAQTVMTSRIVGTTAYMAPEALRGEITPKSDIYSFGVVLLEIITGLPAVDEHREPQLLLDIKEEIEDEEKTIEDYIDKKMNDADSTSVEAMYSVASQCLHEKKNKRPDIKKVQQLLQEMTAS

Purity

>80% by SDS-PAGE and Coomassie blue staining

Predicted Molecular Mass

76.23 kDa.
Disclaimer note: The observed molecular weight of the protein may vary from the listed predicted molecular weight due to post translational modifications, post translation cleavages, relative charges, and other experimental factors.

Activity

This protein was produced in an in vitro wheat germ expression system that should preserve correct conformational folding that is necessary for biological function. While it is possible that this protein could display some level of activity, the functionality of this protein has not been explicitly measured or validated.

Protein / Peptide Type

Recombinant Protein

Scientific Data Images for Recombinant Human IRAK4 GST (N-Term) Protein

SDS-PAGE: Recombinant Human IRAK4 GST (N-Term) Protein [H00051135-P01]

SDS-Page: IRAK4 Recombinant Protein [H00051135-P01]

Functional: Recombinant Human IRAK4 Protein [H00051135-P01]

Formulation, Preparation, and Storage

H00051135-P01

Preparation Method	in vitro wheat germ expression system
Formulation	50 mM Tris-HCl, 10 mM reduced Glutathione, pH 8.0 in the elution buffer.
Preservative	No Preservative
Concentration	Please see the vial label for concentration. If unlisted please contact technical services.
Shipping	The product is shipped with dry ice or equivalent. Upon receipt, store it immediately at the temperature recommended below.
Stability & Storage	Store at -80C. Avoid freeze-thaw cycles.

Background: IRAK4

IRAK4( AAH13316, 1 a.a. - 461 a.a.) recombinant protein with GST.

Long Name

Interleukin-1 Receptor-Associated Kinase 4

Alternate Names

REN64

Gene Symbol

IRAK4

Additional IRAK4 Products

Product Documents for Recombinant Human IRAK4 GST (N-Term) Protein

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Certificate of Analysis

To download a Certificate of Analysis, please enter a lot or batch number in the search box below.

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Product Specific Notices for Recombinant Human IRAK4 GST (N-Term) Protein

This product is produced by and distributed for Abnova, a company based in Taiwan.

This product is for research use only and is not approved for use in humans or in clinical diagnosis. This product is guaranteed for 1 year from date of receipt.

Related Research Areas

IL-1 Family

Intracellular Kinases

NOD-like Receptors and the Inflammasome

Toll-Like Receptors

Translation Regulation

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