IRE1 alpha Antibody (8D12) - Azide and BSA Free

Novus Biologicals | Catalog # H00002081-M02

Novus Biologicals
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Key Product Details

Species Reactivity

Human

Applications

Western Blot, ELISA, Immunocytochemistry/ Immunofluorescence, Proximity Ligation Assay

Label

Unconjugated

Antibody Source

Monoclonal Mouse IgG2a Kappa Clone # 8D12

Format

Azide and BSA Free
View all IRE1 alpha Primary Antibodies »

Product Specifications

Immunogen

This IRE1 alpha antibody (8D12) was raised against ERN1 (AAC25991, 401 a.a. ~ 500 a.a) partial recombinant protein with GST tag. MW of the GST tag alone is 26 KDa. EEVINLVDQTSENAPTTVSRDVEEKPAHAPARPEAPVDSMLKDMATIILSTFLLIGWVAFIITYPLSMHQQQQLQHQQFQKELEKIQLLQQQQQQLPFHP

Clonality

Monoclonal

Host

Mouse

Isotype

IgG2a Kappa

Theoretical MW

110 kDa.
Disclaimer note: The observed molecular weight of the protein may vary from the listed predicted molecular weight due to post translational modifications, post translation cleavages, relative charges, and other experimental factors.

Description

Quality control test: Antibody Reactive Against Recombinant Protein.

Scientific Data Images for IRE1 alpha Antibody (8D12) - Azide and BSA Free

Western Blot: IRE1 alpha Antibody (8D12) [H00002081-M02]

Western Blot: IRE1 alpha Antibody (8D12) [H00002081-M02]

Western Blot: IRE1 alpha Antibody (8D12) [H00002081-M02] - IRE1 Antibody (8D12) [H00002081-M02] - ERN1 monoclonal antibody (M02), clone 8D12. Analysis of ERN1 expression in LNCaP. Theoretical molecular weight: 110 kDa.
Immunocytochemistry/ Immunofluorescence: IRE1 alpha Antibody (8D12) [H00002081-M02]

Immunocytochemistry/ Immunofluorescence: IRE1 alpha Antibody (8D12) [H00002081-M02]

Immunocytochemistry/Immunofluorescence: IRE1 alpha Antibody (8D12) [H00002081-M02] - Analysis of monoclonal antibody to ERN1 on HeLa cell. Antibody concentration 25 ug/ml.
Western Blot: IRE1 alpha Antibody (8D12) [H00002081-M02]

Western Blot: IRE1 alpha Antibody (8D12) [H00002081-M02]

Western Blot: IRE1 alpha Antibody (8D12) [H00002081-M02] - Analysis of ERN1 expression in HeLa. Theoretical molecular weight: 110 kDa. Observed molecular weight may vary.
Proximity Ligation Assay: IRE1 alpha Antibody (8D12) [H00002081-M02]

Proximity Ligation Assay: IRE1 alpha Antibody (8D12) [H00002081-M02]

Proximity Ligation Assay: IRE1 alpha Antibody (8D12) [H00002081-M02] - Analysis of protein-protein interactions between TRAF2 and ERN1. HeLa cells were stained with anti-TRAF2 rabbit purified polyclonal 1:1200 and anti-ERN1 mouse monoclonal antibody 1:50. Each red dot represents the detection of protein-protein interaction complex, and nuclei were counterstained with DAPI (blue).
ELISA: IRE1 alpha Antibody (8D12) [H00002081-M02]

ELISA: IRE1 alpha Antibody (8D12) [H00002081-M02]

ELISA: IRE1 alpha Antibody (8D12) [H00002081-M02] - IRE1 Antibody (8D12) [H00002081-M02] - Detection limit for recombinant GST tagged ERN1 is approximately 0.03ng/ml as a capture antibody.

Applications for IRE1 alpha Antibody (8D12) - Azide and BSA Free

Application
Recommended Usage

Immunocytochemistry/ Immunofluorescence

1:10-1:500

Western Blot

1:500
Application Notes
Antibody reactivity against cell lysate and recombinant protein for WB. It has also been used for IF and ELISA.

Formulation, Preparation, and Storage

Purification

IgG purified

Formulation

In 1x PBS, pH 7.4

Format

Azide and BSA Free

Preservative

No Preservative

Concentration

Concentrations vary lot to lot. See vial label for concentration. If unlisted please contact technical services.

Shipping

The product is shipped with polar packs. Upon receipt, store it immediately at the temperature recommended below.

Stability & Storage

Aliquot and store at -20C or -80C. Avoid freeze-thaw cycles.

Background: IRE1 alpha

Accumulation of misfolded proteins in the endoplasmic reticulum (ER) activates the unfolded protein response (UPR) and upregulates ER molecular chaperones in order to cope with ER stress. UPR is initiated by three ER-localized protein sensors: PERK (PKR-like ER kinase), ATF (activating transcription factor 6), and IRE1 alpha (inositol-requiring enzyme 1 alpha) (1). IRE1 alpha is correlated with X-box binding protein (XBP1) as a potent UPR transcriptional activator (2).

Transcriptional activation of UPR-responsive genes are regulated by the ATF6 and IRE1-XBP1 pathways, which are often regulated by HIFs and contribute to cell survival under ER hypoxic stress (3). UPR signaling can a) inhibit protein translation to restore cell function, b) activate signaling to increase production of molecular chaperones for protein folding, and c) initiate ubiquitination signaling that leads to degradation of misfolded proteins in ER.

IRE1 alpha acts as the sensor of unfolded proteins in the ER. IRE1 alpha not only promotes cell survival but can initiate apoptosis when accumulation of unfolded proteins in the ER causes stress. IRE1 alpha is essential for viability under stress conditions that cause unfolded proteins to accumulate in the ER. IRE1 alpha is a transmembrane protein that has both serine-threonine kinase and endoribonuclease activities and has a theoretical molecular weight of 110 kDa. When detecting phospho-IRE1 alpha, it is recommended to normalize its band intensity with total IRE1 alpha.

References

1. Zheng, W., Xie, W., Yin, D., Luo, R., Liu, M., & Guo, F. (2019). ATG5 and ATG7 induced autophagy interplays with UPR via PERK signaling. Cell Commun Signal, 17(1), 42. doi:10.1186/s12964-019-0353-3

2. Cho, Y. M., Kim, D. H., Lee, K. H., Jeong, S. W., & Kwon, O. J. (2018). The IRE1alpha-XBP1s pathway promotes insulin-stimulated glucose uptake in adipocytes by increasing PPARgamma activity. Exp Mol Med, 50(8), 102. doi:10.1038/s12276-018-0131-0

3. Xia, Z., Wu, S., Wei, X., Liao, Y., Yi, P., Liu, Y.,... Liu, J. (2019). Hypoxic ER stress suppresses beta-catenin expression and promotes cooperation between the transcription factors XBP1 and HIF1alpha for cell survival. J Biol Chem, 294(37), 13811-13821. doi:10.1074/jbc.RA119.008353

Long Name

Serine/threonine-protein kinase/endoribonuclease IRE1

Alternate Names

ERN1, hIRE1p, IRE1, Ire1-alpha

Entrez Gene IDs

2081 (Human)

Gene Symbol

ERN1

OMIM

604033 (Human)

UniProt

AAC25991 (Human)

Additional IRE1 alpha Products

Product Documents for IRE1 alpha Antibody (8D12) - Azide and BSA Free

Certificate of Analysis

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Product Specific Notices for IRE1 alpha Antibody (8D12) - Azide and BSA Free

This product is produced by and distributed for Abnova, a company based in Taiwan.

This product is for research use only and is not approved for use in humans or in clinical diagnosis. Primary Antibodies are guaranteed for 1 year from date of receipt.

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Protocols

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FAQs

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