IRF2 Antibody (JJ088-0)
Novus Biologicals | Catalog # NBP2-67870
Recombinant Monoclonal Antibody
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Key Product Details
Species Reactivity
Human, Mouse
Applications
Western Blot, Immunocytochemistry/ Immunofluorescence
Label
Unconjugated
Antibody Source
Recombinant Monoclonal Rabbit IgG Clone # JJ088-0 expressed in HEK293
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Product Specifications
Immunogen
Synthetic peptide within C-terminal human IRF2. (SwissProt: P14316 Human; SwissProt: P23906 Mouse)
Localization
Nucleus.
Clonality
Monoclonal
Host
Rabbit
Isotype
IgG
Scientific Data Images for IRF2 Antibody (JJ088-0)
Western Blot: IRF2 Antibody (JJ088-0) [NBP2-67870]
Western Blot: IRF2 Antibody (JJ088-0) [NBP2-67870] - Western blot analysis of IRF2 on human lung tissue lysates. Proteins were transferred to a PVDF membrane and blocked with 5% BSA in PBS for 1 hour at room temperature. The primary antibody (1/500) was used in 5% BSA at room temperature for 2 hours. Goat AImmunocytochemistry/ Immunofluorescence: IRF2 Antibody (JJ088-0) [NBP2-67870]
Immunocytochemistry/Immunofluorescence: IRF2 Antibody (JJ088-0) [NBP2-67870] - Staining IRF2 in PANC-1 cells (green). The nuclear counter stain is DAPI (blue). Cells were fixed in paraformaldehyde, permeabilised with 0.25% Triton X100/PBS.Western Blot: IRF2 Antibody (JJ088-0) [NBP2-67870]
Western Blot: IRF2 Antibody (JJ088-0) [NBP2-67870] - Analysis of IRF2 on human lung lysates using anti-IRF2 antibody at 1/1,000 dilution.Immunocytochemistry/ Immunofluorescence: IRF2 Antibody (JJ088-0) [NBP2-67870]
Immunocytochemistry/Immunofluorescence: IRF2 Antibody (JJ088-0) [NBP2-67870] - Staining IRF2 in A549 cells (green). The nuclear counter stain is DAPI (blue). Cells were fixed in paraformaldehyde, permeabilised with 0.25% Triton X100/PBS.Immunocytochemistry/ Immunofluorescence: IRF2 Antibody (JJ088-0) [NBP2-67870]
Immunocytochemistry/Immunofluorescence: IRF2 Antibody (JJ088-0) [NBP2-67870] - Staining IRF2 in Hela cells (green). The nuclear counter stain is DAPI (blue). Cells were fixed in paraformaldehyde, permeabilised with 0.25% Triton X100/PBS.Applications for IRF2 Antibody (JJ088-0)
Application
Recommended Usage
Immunocytochemistry/ Immunofluorescence
1:50-1:200
Western Blot
1:500-1:2000
Formulation, Preparation, and Storage
Purification
Protein A purified
Formulation
TBS (pH7.4), 0.05% BSA, 40% Glycerol
Preservative
0.05% Sodium Azide
Concentration
1 mg/ml
Shipping
The product is shipped with polar packs. Upon receipt, store it immediately at the temperature recommended below.
Stability & Storage
Store at 4C short term. Aliquot and store at -20C long term. Avoid freeze-thaw cycles.
Background: IRF2
Long Name
Interferon Regulatory Factor 2
Alternate Names
DKFZp686F0244, interferon regulatory factor 2, IRF-2
Gene Symbol
IRF2
Additional IRF2 Products
Product Documents for IRF2 Antibody (JJ088-0)
Certificate of Analysis
To download a Certificate of Analysis, please enter a lot or batch number in the search box below.
Product Specific Notices for IRF2 Antibody (JJ088-0)
This product is for research use only and is not approved for use in humans or in clinical diagnosis. Primary Antibodies are guaranteed for 1 year from date of receipt.
Related Research Areas
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Protocols
Find general support by application which include: protocols, troubleshooting, illustrated assays, videos and webinars.
- Appropriate Fixation of IHC/ICC Samples
- Cellular Response to Hypoxia Protocols
- ClariTSA™ Fluorophore Kits
- Detection & Visualization of Antibody Binding
- ICC Cell Smear Protocol for Suspension Cells
- ICC Immunocytochemistry Protocol Videos
- ICC for Adherent Cells
- Immunocytochemistry (ICC) Protocol
- Immunocytochemistry Troubleshooting
- Immunofluorescence of Organoids Embedded in Cultrex Basement Membrane Extract
- Immunohistochemistry (IHC) and Immunocytochemistry (ICC) Protocols
- Preparing Samples for IHC/ICC Experiments
- Preventing Non-Specific Staining (Non-Specific Binding)
- Primary Antibody Selection & Optimization
- Protocol for VisUCyte™ HRP Polymer Detection Reagent
- Protocol for the Fluorescent ICC Staining of Cell Smears - Graphic
- Protocol for the Fluorescent ICC Staining of Cultured Cells on Coverslips - Graphic
- Protocol for the Preparation and Fluorescent ICC Staining of Cells on Coverslips
- Protocol for the Preparation and Fluorescent ICC Staining of Non-adherent Cells
- Protocol for the Preparation and Fluorescent ICC Staining of Stem Cells on Coverslips
- Protocol for the Preparation of a Cell Smear for Non-adherent Cell ICC - Graphic
- R&D Systems Quality Control Western Blot Protocol
- TUNEL and Active Caspase-3 Detection by IHC/ICC Protocol
- The Importance of IHC/ICC Controls
- Troubleshooting Guide: Western Blot Figures
- Western Blot Conditions
- Western Blot Protocol
- Western Blot Protocol for Cell Lysates
- Western Blot Troubleshooting
- Western Blot Troubleshooting Guide
- View all Protocols, Troubleshooting, Illustrated assays and Webinars
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