IRF3 Antibody (PCRP-IRF3-1E6)

Novus Biologicals | Catalog # NBP3-07529

Novus Biologicals
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Key Product Details

Species Reactivity

Human

Applications

Western Blot, Flow Cytometry, Immunocytochemistry/ Immunofluorescence, Protein Array

Label

Unconjugated

Antibody Source

Monoclonal Mouse IgG2A Clone # PCRP-IRF3-1E6
View all IRF3 Primary Antibodies »

Product Specifications

Immunogen

Recombinant full-length human IRF3 protein (Uniprot: Q14653)

Localization

Cytoplasm. Nucleus. Shuttles between cytoplasmic and nuclear compartments, with export being the prevailing effect. When activated, IRF3 interaction with CREBBP prevents its export to the cytoplasm.

Clonality

Monoclonal

Host

Mouse

Isotype

IgG2A

Theoretical MW

50 kDa.
Disclaimer note: The observed molecular weight of the protein may vary from the listed predicted molecular weight due to post translational modifications, post translation cleavages, relative charges, and other experimental factors.

Description

200ug/ml of antibody purified from Bioreactor Concentrate by Protein A or G. Prepared in 10 mM PBS with 0.05% BSA & 0.05% azide. Also available WITHOUT BSA & azide at 1.0 mg/ml. (NBP3-08854)

Antibody with azide - store at 2 to 8C. Antibody without azide - store at -20 to -80 C.

Scientific Data Images for IRF3 Antibody (PCRP-IRF3-1E6)

Immunocytochemistry/ Immunofluorescence: IRF3 Antibody (PCRP-IRF3-1E6) [NBP3-07529]

Immunocytochemistry/ Immunofluorescence: IRF3 Antibody (PCRP-IRF3-1E6) [NBP3-07529]

Immunocytochemistry/Immunofluorescence: IRF3 Antibody (PCRP-IRF3-1E6) [NBP3-07529] - Immunofluorescence Analysis of PFA-fixed HeLa cells using IRF3 Mouse Monoclonal Antibody (PCRP-IRF3-1E6) followed by goat anti- Mouse IgG-CF488 (green). Counterstain is phalloidin.
Flow Cytometry: IRF3 Antibody (PCRP-IRF3-1E6) [NBP3-07529]

Flow Cytometry: IRF3 Antibody (PCRP-IRF3-1E6) [NBP3-07529]

Flow Cytometry: IRF3 Antibody (PCRP-IRF3-1E6) [NBP3-07529] - Flow Cytometric Analysis of PFA-fixed HeLa cells. IRF3 Mouse Monoclonal Antibody (PCRP-IRF3-1E6) followed by goat anti- Mouse IgG-CF488 (blue), isotype control (red).
Protein Array: IRF3 Antibody (PCRP-IRF3-1E6) [NBP3-07529]

Protein Array: IRF3 Antibody (PCRP-IRF3-1E6) [NBP3-07529]

Protein Array: IRF3 Antibody (PCRP-IRF3-1E6) [NBP3-07529] - Analysis of Protein Array containing more than 19,000 full-length human proteins using IRF3 Mouse Monoclonal Antibody (PCRP-IRF3-1E6).

Applications for IRF3 Antibody (PCRP-IRF3-1E6)

Application
Recommended Usage

Flow Cytometry

1-2 ug/million cells

Immunocytochemistry/ Immunofluorescence

1-2 ug/ml

Western Blot

1-2 ug/ml
Application Notes
Optimal dilution for a specific application should be determined.

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Flow Cytometry

Formulation, Preparation, and Storage

Purification

Protein A or G purified

Formulation

10 mM PBS with 0.05% BSA

Preservative

0.05% Sodium Azide

Concentration

0.2 mg/ml

Shipping

The product is shipped with polar packs. Upon receipt, store it immediately at the temperature recommended below.

Stability & Storage

Store at 4C.

Background: IRF3

IRF3 is a family of interferon (IFN) regulatory factors (IRFs) have been shown to play a role in transcription of IFN genes as well as IFN-stimulated genes. The IRF-3 gene encodes a 50-kDa protein that binds specifically to the IFN-stimulated response element (ISRE) but not to the IRF-1 binding site PRD-I. Overexpression of IRF-3 stimulates expression of the IFN-stimulated gene 15 (ISG15) promoter, an ISRE-containing promoter. he high amino acid homology between IRF-3 and ISG factor 3 gamma polypeptide (ISGF3 gamma) and their similar binding properties indicate that, like ISGF3 gamma, IRF-3 may activate transcription by complex formation with other transcriptional factors, possibly members of the Stat family (1). IRF-3 is expressed constitutively in a variety of tissues, and the relative levels of IRF-3 mRNA do not change in virus-infected or IFN-treated cells. (2). In uninfected cells, the IRF-3 component of DRAF1 resides in the cytoplasm. The cytoplasmic localization of IRF-3 is dependent on a nuclear export signal, and IRF-3 is recognized by the chromosome region maintenance 1 (CRM1) (also known as exportin 1) shuttling receptor. Following infection and specific phosphorylation, IRF-3 accumulates in the nucleus where it associates with CBP and p300 (3).

Long Name

Interferon Regulatory Factor 3

Alternate Names

interferon regulatory factor 3, IRF-3

Gene Symbol

IRF3

UniProt

Q14653

Additional IRF3 Products

Product Documents for IRF3 Antibody (PCRP-IRF3-1E6)

Certificate of Analysis

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Product Specific Notices for IRF3 Antibody (PCRP-IRF3-1E6)

This product is for research use only and is not approved for use in humans or in clinical diagnosis. Primary Antibodies are guaranteed for 1 year from date of receipt.

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Protocols

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FAQs

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