IRF3 Antibody (PCRP-IRF3-3B2) - Azide and BSA Free

Novus Biologicals | Catalog # NBP3-14106

Novus Biologicals
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Key Product Details

Species Reactivity

Human

Applications

Western Blot, Immunocytochemistry/ Immunofluorescence, Protein Array

Label

Unconjugated

Antibody Source

Monoclonal Mouse IgG1 kappa Clone # PCRP-IRF3-3B2

Format

Azide and BSA Free
View all IRF3 Primary Antibodies »

Product Specifications

Immunogen

Recombinant full-length human IRF3 protein (Uniprot: Q14653)

Localization

Cytoplasm, Nucleus. Shuttles between cytoplasmic and nuclear compartments, with export being the prevailing effect, When activated, IRF3 interaction with CREBBP prevents its export to the cytoplasm

Clonality

Monoclonal

Host

Mouse

Isotype

IgG1 kappa

Theoretical MW

50 kDa.
Disclaimer note: The observed molecular weight of the protein may vary from the listed predicted molecular weight due to post translational modifications, post translation cleavages, relative charges, and other experimental factors.

Description

1.0 mg/ml of antibody purified from Bioreactor Concentrate by Protein A/G. Prepared in 10mM PBS WITHOUT BSA & azide. Also available at 200 ug/ml WITH BSA & azide (NBP3-13954).

Antibody with azide - store at 2 to 8C. Antibody without azide - store at -20 to -80 C.

Scientific Data Images for IRF3 Antibody (PCRP-IRF3-3B2) - Azide and BSA Free

Protein Array: IRF3 Antibody (PCRP-IRF3-3B2) - Azide and BSA Free [NBP3-14106]

Protein Array: IRF3 Antibody (PCRP-IRF3-3B2) - Azide and BSA Free [NBP3-14106]

Protein Array: IRF3 Antibody (PCRP-IRF3-3B2) - Azide and BSA Free [NBP3-14106] - Analysis of Protein Array containing more than 19,000 full-length human proteins using IRF3 Antibody (PCRP-IRF3-3B2).
IRF3 Antibody (PCRP-IRF3-3B2) - Azide and BSA Free Flow Cytometry: IRF3 Antibody (PCRP-IRF3-3B2) - Azide and BSA Free [NBP3-14106] -

Flow Cytometry: IRF3 Antibody (PCRP-IRF3-3B2) - Azide and BSA Free [NBP3-14106] -

Flow cytometric analysis of PFA-fixed HeLa cells. IRF3 Antibody (PCRP-IRF3-3B2) - Azide and BSA Free followed by goat anti-mouse IgG-CF488 (blue); isotype control (red).
IRF3 Antibody (PCRP-IRF3-3B2) - Azide and BSA Free Immunocytochemistry/ Immunofluorescence: IRF3 Antibody (PCRP-IRF3-3B2) - Azide and BSA Free [NBP3-14106] -

Immunocytochemistry/ Immunofluorescence: IRF3 Antibody (PCRP-IRF3-3B2) - Azide and BSA Free [NBP3-14106] -

Immunofluorescence Analysis of PFA-fixed HeLa cells stained using IRF3 Antibody (PCRP-IRF3-3B2) - Azide and BSA Free followed by goat anti-mouse IgG-CF488 (green). CF640A phalloidin (red).
IRF3 Antibody (PCRP-IRF3-3B2) - Azide and BSA Free Western Blot: IRF3 Antibody (PCRP-IRF3-3B2) - Azide and BSA Free [NBP3-14106] -

Western Blot: IRF3 Antibody (PCRP-IRF3-3B2) - Azide and BSA Free [NBP3-14106] -

Western Blot Analysis of HeLa cell lysate using IRF3 Antibody (PCRP-IRF3-3B2) - Azide and BSA Free.
IRF3 Antibody (PCRP-IRF3-3B2) - Azide and BSA Free Western Blot: IRF3 Antibody (PCRP-IRF3-3B2) - Azide and BSA Free [NBP3-14106] -

Western Blot: IRF3 Antibody (PCRP-IRF3-3B2) - Azide and BSA Free [NBP3-14106] -

Western Blot Analysis of HeLa cell lysate using IRF3 Antibody (PCRP-IRF3-3B2) - Azide and BSA Free.

Applications for IRF3 Antibody (PCRP-IRF3-3B2) - Azide and BSA Free

Application
Recommended Usage

Immunocytochemistry/ Immunofluorescence

1-2 ug/ml

Western Blot

2-4 ug/ml

Formulation, Preparation, and Storage

Purification

Protein A or G purified

Formulation

10mM PBS

Format

Azide and BSA Free

Preservative

No Preservative

Concentration

1 mg/ml

Shipping

The product is shipped with polar packs. Upon receipt, store it immediately at the temperature recommended below.

Stability & Storage

Store at -20 to -80C. Avoid freeze-thaw cycles.

Background: IRF3

IRF3 is a family of interferon (IFN) regulatory factors (IRFs) have been shown to play a role in transcription of IFN genes as well as IFN-stimulated genes. The IRF-3 gene encodes a 50-kDa protein that binds specifically to the IFN-stimulated response element (ISRE) but not to the IRF-1 binding site PRD-I. Overexpression of IRF-3 stimulates expression of the IFN-stimulated gene 15 (ISG15) promoter, an ISRE-containing promoter. he high amino acid homology between IRF-3 and ISG factor 3 gamma polypeptide (ISGF3 gamma) and their similar binding properties indicate that, like ISGF3 gamma, IRF-3 may activate transcription by complex formation with other transcriptional factors, possibly members of the Stat family (1). IRF-3 is expressed constitutively in a variety of tissues, and the relative levels of IRF-3 mRNA do not change in virus-infected or IFN-treated cells. (2). In uninfected cells, the IRF-3 component of DRAF1 resides in the cytoplasm. The cytoplasmic localization of IRF-3 is dependent on a nuclear export signal, and IRF-3 is recognized by the chromosome region maintenance 1 (CRM1) (also known as exportin 1) shuttling receptor. Following infection and specific phosphorylation, IRF-3 accumulates in the nucleus where it associates with CBP and p300 (3).

Long Name

Interferon Regulatory Factor 3

Alternate Names

interferon regulatory factor 3, IRF-3

Gene Symbol

IRF3

Additional IRF3 Products

Product Documents for IRF3 Antibody (PCRP-IRF3-3B2) - Azide and BSA Free

Certificate of Analysis

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Product Specific Notices for IRF3 Antibody (PCRP-IRF3-3B2) - Azide and BSA Free

This product is for research use only and is not approved for use in humans or in clinical diagnosis. Primary Antibodies are guaranteed for 1 year from date of receipt.

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Protocols

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FAQs

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