IRF5 Antibody (1H6) - Azide and BSA Free

Novus Biologicals | Catalog # H00003663-M03

Novus Biologicals
100% Guarantee

Key Product Details

Species Reactivity

Validated:

Human, Mouse

Cited:

Human

Applications

Validated:

Western Blot, ELISA, Flow Cytometry, Immunocytochemistry/ Immunofluorescence, Immunoprecipitation

Cited:

Flow Cytometry

Label

Unconjugated

Antibody Source

Monoclonal Mouse IgG1 kappa Clone # 1H6

Format

Azide and BSA Free
View all IRF5 Primary Antibodies »

Product Specifications

Immunogen

IRF5 (NP_002191, 395 a.a. ~ 504 a.a) partial recombinant protein with GST tag. MW of the GST tag alone is 26 KDa. TPPPFEIFFCFGEEWPDRKPREKKLITVQVVPVAARLLLEMFSGELSWSADSIRLQISNPDLKDRMVEQFKELHHIWQSQQRLQPVAQAPPGAGLGVGQGPWPMHPAGMQ

Reactivity Notes

Please note that this antibody is reactive to Mouse and derived from the same host, Mouse. Additional Mouse on Mouse blocking steps may be required for IHC and ICC experiments. Please contact Technical Support for more information.

Specificity

IRF5 - interferon regulatory factor 5

Clonality

Monoclonal

Host

Mouse

Isotype

IgG1 kappa

Description

Quality control test: Antibody Reactive Against Recombinant Protein.

Scientific Data Images for IRF5 Antibody (1H6) - Azide and BSA Free

Western Blot: IRF5 Antibody (1H6) [H00003663-M03]

Western Blot: IRF5 Antibody (1H6) [H00003663-M03]

Western Blot: IRF5 Antibody (1H6) [H00003663-M03] - Analysis of IRF5 expression in NIH/3T3 (Cat # L018V1).
Immunocytochemistry/ Immunofluorescence: IRF5 Antibody (1H6) [H00003663-M03]

Immunocytochemistry/ Immunofluorescence: IRF5 Antibody (1H6) [H00003663-M03]

Immunocytochemistry/Immunofluorescence: IRF5 Antibody (1H6) [H00003663-M03] - Analysis of monoclonal antibody to IRF5 on A-431 cell. Antibody concentration 10 ug/ml
Western Blot: IRF5 Antibody (1H6) [H00003663-M03]

Western Blot: IRF5 Antibody (1H6) [H00003663-M03]

Western Blot: IRF5 Antibody (1H6) [H00003663-M03] - Analysis of IRF5 expression in A-431 (Cat # L015V1).
ELISA: IRF5 Antibody (1H6) [H00003663-M03]

ELISA: IRF5 Antibody (1H6) [H00003663-M03]

ELISA: IRF5 Antibody (1H6) [H00003663-M03] - Detection limit for recombinant GST tagged IRF5 is approximately 0.1ng/ml as a capture antibody.
Immunoprecipitation: IRF5 Antibody (1H6) [H00003663-M03]

Immunoprecipitation: IRF5 Antibody (1H6) [H00003663-M03]

Immunoprecipitation: IRF5 Antibody (1H6) [H00003663-M03] - Analysis of IRF5 transfected lysate using anti-IRF5 monoclonal antibody and Protein A Magnetic Bead, and immunoblotted with IRF5 mouse polyclonal antibody.

Applications for IRF5 Antibody (1H6) - Azide and BSA Free

Application
Recommended Usage

Western Blot

1:500
Application Notes
Antibody reactivity against cell lysate and recombinant protein for WB. It has also been used for IF and ELISA. Use in FLOW reported in scientific literature (PMID: 30593537).

Flow Cytometry Panel Builder

Bio-Techne Knows Flow Cytometry

Save time and reduce costly mistakes by quickly finding compatible reagents using the Panel Builder Tool.

Advanced Features

  • Spectra Viewer - Custom analysis of spectra from multiple fluorochromes
  • Spillover Popups - Visualize the spectra of individual fluorochromes
  • Antigen Density Selector - Match fluorochrome brightness with antigen density
Build Your Panel Now
Flow Cytometry

Formulation, Preparation, and Storage

Purification

IgG purified

Formulation

In 1x PBS, pH 7.4

Format

Azide and BSA Free

Preservative

No Preservative

Concentration

Concentrations vary lot to lot. See vial label for concentration. If unlisted please contact technical services.

Shipping

The product is shipped with polar packs. Upon receipt, store it immediately at the temperature recommended below.

Stability & Storage

Aliquot and store at -20C or -80C. Avoid freeze-thaw cycles.

Background: IRF5

This gene encodes a member of the interferon regulatory factor (IRF) family, a group of transcription factors with diverse roles, including virus-mediated activation of interferon, and modulation of cell growth, differentiation, apoptosis, and immune system activity. Members of the IRF family are characterized by a conserved N-terminal DNA-binding domain containing tryptophan (W) repeats. Alternative splice variants encoding different isoforms exist.

Long Name

Interferon Regulatory Factor 5

Alternate Names

IBD14, interferon regulatory factor 5, IRF-5, SLEB10

Entrez Gene IDs

3663 (Human)

Gene Symbol

IRF5

OMIM

607218 (Human)

UniProt

NP_002191

Additional IRF5 Products

Product Documents for IRF5 Antibody (1H6) - Azide and BSA Free

Certificate of Analysis

To download a Certificate of Analysis, please enter a lot or batch number in the search box below.

Download SDS

Product Specific Notices for IRF5 Antibody (1H6) - Azide and BSA Free

This product is produced by and distributed for Abnova, a company based in Taiwan.

This product is for research use only and is not approved for use in humans or in clinical diagnosis. Primary Antibodies are guaranteed for 1 year from date of receipt.

Related Research Areas

Macrophage Activation Markers

Citations for IRF5 Antibody (1H6) - Azide and BSA Free

Customer Reviews for IRF5 Antibody (1H6) - Azide and BSA Free

There are currently no reviews for this product. Be the first to review IRF5 Antibody (1H6) - Azide and BSA Free and earn rewards!

Have you used IRF5 Antibody (1H6) - Azide and BSA Free?

Submit a review and receive an Amazon gift card!

$25/€18/£15/$25CAN/¥2500 Yen for a review with an image

$10/€7/£6/$10CAN/¥1110 Yen for a review without an image

Submit a review
Amazon Gift Card

Protocols

Find general support by application which include: protocols, troubleshooting, illustrated assays, videos and webinars.

FAQs

No product specific FAQs exist for this product.

View all FAQs for Antibodies