IRF5 Antibody (SD203-07)
Novus Biologicals | Catalog # NBP2-67742
Recombinant Monoclonal Antibody
![Western Blot: IRF5 Antibody (SD203-07) [NBP2-67742]](https://resources.rndsystems.com/images/products/IRF5-Antibody-SD203-07-Western-Blot-NBP2-67742-img0004.jpg "Western Blot: IRF5 Antibody (SD203-07) [NBP2-67742]")
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Key Product Details
Species Reactivity
Human, Mouse, Rat
Applications
Immunohistochemistry, Immunohistochemistry-Paraffin, Western Blot, Flow Cytometry, Immunocytochemistry/ Immunofluorescence, Simple Western, Immunoprecipitation
Label
Unconjugated
Antibody Source
Recombinant Monoclonal Rabbit IgG Clone # SD203-07 expressed in HEK293
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Product Specifications
Immunogen
Recombinant protein within human IRF5 aa 1-200. (SwissProt: Q13568 Human; SwissProt: P56477 Mouse; Unigene:203787 Rat)
Localization
Nucleus, Cytoplasm.
Clonality
Monoclonal
Host
Rabbit
Isotype
IgG
Scientific Data Images for IRF5 Antibody (SD203-07)
Western Blot: IRF5 Antibody (SD203-07) [NBP2-67742]
Western Blot: IRF5 Antibody (SD203-07) [NBP2-67742] - Analysis of IRF5 on THP-1 cells lysates using anti-IRF5 antibody at 1/1,000 dilution.![Immunocytochemistry/ Immunofluorescence: IRF5 Antibody (SD203-07) [NBP2-67742]](https://resources.rndsystems.com/images/products/IRF5-Antibody-SD203-07-Immunocytochemistry-Immunofluorescence-NBP2-67742-img0005.jpg "Immunocytochemistry/ Immunofluorescence: IRF5 Antibody (SD203-07) [NBP2-67742]")
Immunocytochemistry/ Immunofluorescence: IRF5 Antibody (SD203-07) [NBP2-67742]
Immunocytochemistry/Immunofluorescence: IRF5 Antibody (SD203-07) [NBP2-67742] - Staining IRF5 in RH-35 cells (green). The nuclear counter stain is DAPI (blue). Cells were fixed in paraformaldehyde, permeabilised with 0.25% Triton X100/PBS.![Immunohistochemistry-Paraffin: IRF5 Antibody (SD203-07) [NBP2-67742]](https://resources.rndsystems.com/images/products/IRF5-Antibody-SD203-07-Immunohistochemistry-Paraffin-NBP2-67742-img0003.jpg "Immunohistochemistry-Paraffin: IRF5 Antibody (SD203-07) [NBP2-67742]")
Immunohistochemistry-Paraffin: IRF5 Antibody (SD203-07) [NBP2-67742]
Immunohistochemistry-Paraffin: IRF5 Antibody (SD203-07) [NBP2-67742] - Analysis of paraffin-embedded human spleen tissue using anti-IRF5 antibody. Counter stained with hematoxylin.![Flow Cytometry: IRF5 Antibody (SD203-07) [NBP2-67742]](https://resources.rndsystems.com/images/products/IRF5-Antibody-SD203-07-Flow-Cytometry-NBP2-67742-img0001.jpg "Flow Cytometry: IRF5 Antibody (SD203-07) [NBP2-67742]")
Flow Cytometry: IRF5 Antibody (SD203-07) [NBP2-67742]
Flow Cytometry: IRF5 Antibody (SD203-07) [NBP2-67742] - Analysis of Hela cells with IRF5 antibody at 1/50 dilution (red) compared with an unlabelled control (cells without incubation with primary antibody; black). Alexa Fluor 488-conjugated goat anti rabbit IgG was used as the secondary antibody.![Immunocytochemistry/ Immunofluorescence: IRF5 Antibody (SD203-07) [NBP2-67742]](https://resources.rndsystems.com/images/products/IRF5-Antibody-SD203-07-Immunocytochemistry-Immunofluorescence-NBP2-67742-img0002.jpg "Immunocytochemistry/ Immunofluorescence: IRF5 Antibody (SD203-07) [NBP2-67742]")
Immunocytochemistry/ Immunofluorescence: IRF5 Antibody (SD203-07) [NBP2-67742]
Immunocytochemistry/Immunofluorescence: IRF5 Antibody (SD203-07) [NBP2-67742] - Staining IRF5 in HepG2 cells (green). The nuclear counter stain is DAPI (blue). Cells were fixed in paraformaldehyde, permeabilised with 0.25% Triton X100/PBS.Applications for IRF5 Antibody (SD203-07)
Application
Recommended Usage
Flow Cytometry
1:50-1:100
Immunocytochemistry/ Immunofluorescence
1:50-1:200
Immunohistochemistry-Paraffin
1:50-1:200
Simple Western
1:50
Western Blot
1:500-1:2000
Flow Cytometry Panel Builder
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Advanced Features
- Spectra Viewer - Custom analysis of spectra from multiple fluorochromes
- Spillover Popups - Visualize the spectra of individual fluorochromes
- Antigen Density Selector - Match fluorochrome brightness with antigen density
Formulation, Preparation, and Storage
Purification
Protein A purified
Formulation
TBS (pH7.4), 0.05% BSA, 40% Glycerol
Preservative
0.05% Sodium Azide
Concentration
1 mg/ml
Shipping
The product is shipped with polar packs. Upon receipt, store it immediately at the temperature recommended below.
Stability & Storage
Store at 4C short term. Aliquot and store at -20C long term. Avoid freeze-thaw cycles.
Background: IRF5
Long Name
Interferon Regulatory Factor 5
Alternate Names
IBD14, interferon regulatory factor 5, IRF-5, SLEB10
Gene Symbol
IRF5
Additional IRF5 Products
Product Documents for IRF5 Antibody (SD203-07)
Certificate of Analysis
To download a Certificate of Analysis, please enter a lot or batch number in the search box below.
Product Specific Notices for IRF5 Antibody (SD203-07)
This product is for research use only and is not approved for use in humans or in clinical diagnosis. Primary Antibodies are guaranteed for 1 year from date of receipt.
Related Research Areas
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Protocols
Find general support by application which include: protocols, troubleshooting, illustrated assays, videos and webinars.
- 7-Amino Actinomycin D (7-AAD) Cell Viability Flow Cytometry Protocol
- Antigen Retrieval Protocol (PIER)
- Antigen Retrieval for Frozen Sections Protocol
- Appropriate Fixation of IHC/ICC Samples
- Cellular Response to Hypoxia Protocols
- Chromogenic IHC Staining of Formalin-Fixed Paraffin-Embedded (FFPE) Tissue Protocol
- Chromogenic Immunohistochemistry Staining of Frozen Tissue
- ClariTSA™ Fluorophore Kits
- Detection & Visualization of Antibody Binding
- Extracellular Membrane Flow Cytometry Protocol
- Flow Cytometry Protocol for Cell Surface Markers
- Flow Cytometry Protocol for Staining Membrane Associated Proteins
- Flow Cytometry Staining Protocols
- Flow Cytometry Troubleshooting Guide
- Fluorescent IHC Staining of Frozen Tissue Protocol
- Graphic Protocol for Heat-induced Epitope Retrieval
- Graphic Protocol for the Preparation and Fluorescent IHC Staining of Frozen Tissue Sections
- Graphic Protocol for the Preparation and Fluorescent IHC Staining of Paraffin-embedded Tissue Sections
- Graphic Protocol for the Preparation of Gelatin-coated Slides for Histological Tissue Sections
- ICC Cell Smear Protocol for Suspension Cells
- ICC Immunocytochemistry Protocol Videos
- ICC for Adherent Cells
- IHC Sample Preparation (Frozen sections vs Paraffin)
- Immunocytochemistry (ICC) Protocol
- Immunocytochemistry Troubleshooting
- Immunofluorescence of Organoids Embedded in Cultrex Basement Membrane Extract
- Immunofluorescent IHC Staining of Formalin-Fixed Paraffin-Embedded (FFPE) Tissue Protocol
- Immunohistochemistry (IHC) and Immunocytochemistry (ICC) Protocols
- Immunohistochemistry Frozen Troubleshooting
- Immunohistochemistry Paraffin Troubleshooting
- Immunoprecipitation Protocol
- Intracellular Flow Cytometry Protocol Using Alcohol (Methanol)
- Intracellular Flow Cytometry Protocol Using Detergents
- Intracellular Nuclear Staining Flow Cytometry Protocol Using Detergents
- Intracellular Staining Flow Cytometry Protocol Using Alcohol Permeabilization
- Intracellular Staining Flow Cytometry Protocol Using Detergents to Permeabilize Cells
- Preparing Samples for IHC/ICC Experiments
- Preventing Non-Specific Staining (Non-Specific Binding)
- Primary Antibody Selection & Optimization
- Propidium Iodide Cell Viability Flow Cytometry Protocol
- Protocol for Heat-Induced Epitope Retrieval (HIER)
- Protocol for Liperfluo
- Protocol for Making a 4% Formaldehyde Solution in PBS
- Protocol for VisUCyte™ HRP Polymer Detection Reagent
- Protocol for the Characterization of Human Th22 Cells
- Protocol for the Characterization of Human Th9 Cells
- Protocol for the Fluorescent ICC Staining of Cell Smears - Graphic
- Protocol for the Fluorescent ICC Staining of Cultured Cells on Coverslips - Graphic
- Protocol for the Preparation & Fixation of Cells on Coverslips
- Protocol for the Preparation and Chromogenic IHC Staining of Frozen Tissue Sections
- Protocol for the Preparation and Chromogenic IHC Staining of Frozen Tissue Sections - Graphic
- Protocol for the Preparation and Chromogenic IHC Staining of Paraffin-embedded Tissue Sections
- Protocol for the Preparation and Chromogenic IHC Staining of Paraffin-embedded Tissue Sections - Graphic
- Protocol for the Preparation and Fluorescent ICC Staining of Cells on Coverslips
- Protocol for the Preparation and Fluorescent ICC Staining of Non-adherent Cells
- Protocol for the Preparation and Fluorescent ICC Staining of Stem Cells on Coverslips
- Protocol for the Preparation and Fluorescent IHC Staining of Frozen Tissue Sections
- Protocol for the Preparation and Fluorescent IHC Staining of Paraffin-embedded Tissue Sections
- Protocol for the Preparation of Gelatin-coated Slides for Histological Tissue Sections
- Protocol for the Preparation of a Cell Smear for Non-adherent Cell ICC - Graphic
- Protocol: Annexin V and PI Staining by Flow Cytometry
- Protocol: Annexin V and PI Staining for Apoptosis by Flow Cytometry
- R&D Systems Quality Control Western Blot Protocol
- TUNEL and Active Caspase-3 Detection by IHC/ICC Protocol
- The Importance of IHC/ICC Controls
- Troubleshooting Guide: Fluorokine Flow Cytometry Kits
- Troubleshooting Guide: Immunohistochemistry
- Troubleshooting Guide: Western Blot Figures
- Western Blot Conditions
- Western Blot Protocol
- Western Blot Protocol for Cell Lysates
- Western Blot Troubleshooting
- Western Blot Troubleshooting Guide
- View all Protocols, Troubleshooting, Illustrated assays and Webinars
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