IRF6 Antibody (5D1Z4)

Novus Biologicals | Catalog # NBP3-16155

Recombinant Monoclonal Antibody
Novus Biologicals
100% Guarantee

Key Product Details

Species Reactivity

Human, Mouse, Rat

Applications

Western Blot, Immunocytochemistry/ Immunofluorescence, Immunoprecipitation

Label

Unconjugated

Antibody Source

Recombinant Monoclonal Rabbit IgG Clone # 5D1Z4 expressed in HEK293
View all IRF6 Primary Antibodies »

Product Specifications

Immunogen

A synthetic peptide corresponding to a sequence within amino acids 368-467 of human IRF6 (O14896). PFEIYLCFGEEWPDGKPLERKLILVQVIPVVARMIYEMFSGDFTRSFDSGSVRLQISTPDIKDNIVAQLKQLYRILQTQESWQPMQPTPSMQLPPALPPQ

Clonality

Monoclonal

Host

Rabbit

Isotype

IgG

Scientific Data Images for IRF6 Antibody (5D1Z4)

Western Blot: IRF6 Antibody (5D1Z4) [NBP3-16155]

Western Blot: IRF6 Antibody (5D1Z4) [NBP3-16155]

Western Blot: IRF6 Antibody (5D1Z4) [NBP3-16155] - Western blot analysis of extracts of various cell lines, using IRF6 Rabbit mAb (NBP3-16155) at 1:1000 dilution. Secondary antibody: HRP Goat Anti-Rabbit IgG (H+L) at 1:10000 dilution. Lysates/proteins: 25ug per lane. Blocking buffer: 3% nonfat dry milk in TBST. Detection: ECL Basic Kit. Exposure time: 3min.
Immunoprecipitation: IRF6 Antibody (5D1Z4) [NBP3-16155]

Immunoprecipitation: IRF6 Antibody (5D1Z4) [NBP3-16155]

Immunoprecipitation: IRF6 Antibody (5D1Z4) [NBP3-16155] - Immunoprecipitation analysis of 300ug extracts of HepG2 cells using 3ug IRF6 antibody (NBP3-16155). Western blot was performed from the immunoprecipitate using IRF6 antibody (NBP3-16155) at a dilition of 1:1000.
IRF6 Antibody (5D1Z4)

Immunocytochemistry/ Immunofluorescence: IRF6 Antibody (5D1Z4) [NBP3-16155] -

Immunocytochemistry/ Immunofluorescence: IRF6 Antibody (5D1Z4) [NBP3-16155] - Immunofluorescence analysis of NIH-3T3 cells using IRF6 Rabbit mAb at dilution of 1:100 (40x lens). Secondary antibody: Cy3 Goat Anti-Rabbit IgG (H+L) at 1:500 dilution. Blue: DAPI for nuclear staining.
IRF6 Antibody (5D1Z4)

Immunocytochemistry/ Immunofluorescence: IRF6 Antibody (5D1Z4) [NBP3-16155] -

Immunocytochemistry/ Immunofluorescence: IRF6 Antibody (5D1Z4) [NBP3-16155] - Immunofluorescence analysis of C6 cells using IRF6 Rabbit mAb at dilution of 1:100 (40x lens). Secondary antibody: Cy3 Goat Anti-Rabbit IgG (H+L) at 1:500 dilution. Blue: DAPI for nuclear staining.
Immunocytochemistry/ Immunofluorescence: IRF6 Antibody (5D1Z4) [NBP3-16155]

Immunocytochemistry/ Immunofluorescence: IRF6 Antibody (5D1Z4) [NBP3-16155]

Immunocytochemistry/Immunofluorescence: IRF6 Antibody (5D1Z4) [NBP3-16155] - Immunofluorescence analysis of HeLa cells using IRF6 Rabbit mAb (NBP3-16155) at dilution of 1:100 (40x lens). Blue: DAPI for nuclear staining.

Applications for IRF6 Antibody (5D1Z4)

Application
Recommended Usage

Immunocytochemistry/ Immunofluorescence

1:50 - 1:200

Immunoprecipitation

1:500 - 1:1000

Western Blot

1:500 - 1:1000

Formulation, Preparation, and Storage

Purification

Affinity purified

Formulation

PBS (pH 7.3), 50% glycerol, 0.05% BSA

Preservative

0.02% Sodium Azide

Concentration

Please see the vial label for concentration. If unlisted please contact technical services.

Shipping

The product is shipped with polar packs. Upon receipt, store it immediately at the temperature recommended below.

Stability & Storage

Store at -20C. Avoid freeze-thaw cycles.

Background: IRF6

IRF6 encodes a member of the interferon regulatory transcription factor (IRF) family. Family members share a highly-conserved N-terminal helix-turn-helix DNA-binding domain and a less conserved C-terminal protein-binding domain. Mutations in this gene can cause van der Woude syndrome and popliteal pterygium syndrome. This protein is involved in palate formation. [provided by RefSeq]

Long Name

Interferon Regulatory Factor 8

Alternate Names

LPS, OFC6, PIT, PPS, VWS

Gene Symbol

IRF6

Additional IRF6 Products

Product Documents for IRF6 Antibody (5D1Z4)

Certificate of Analysis

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Product Specific Notices for IRF6 Antibody (5D1Z4)

This product is for research use only and is not approved for use in humans or in clinical diagnosis. Primary Antibodies are guaranteed for 1 year from date of receipt.

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Protocols

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FAQs

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