IRF7 Antibody (SC0617)

Novus Biologicals | Catalog # NBP2-67634

Recombinant Monoclonal Antibody
Novus Biologicals
100% Guarantee

Key Product Details

Species Reactivity

Validated:

Human, Mouse, Rat, Zebrafish

Cited:

Mouse

Applications

Validated:

Immunohistochemistry, Immunohistochemistry-Paraffin, Western Blot, Flow Cytometry, Immunocytochemistry/ Immunofluorescence, Immunoprecipitation

Cited:

Chemotaxis

Label

Unconjugated

Antibody Source

Recombinant Monoclonal Rabbit IgG Clone # SC0617 expressed in HEK293
View all IRF7 Primary Antibodies »

Product Specifications

Immunogen

Synthetic peptide within human IRF7 aa 200-240. (SwissProt: Q92985 Human; SwissProt: P70434 Mouse; Unigene:101159 Rat)

Localization

Nucleus, Cytoplasm.

Clonality

Monoclonal

Host

Rabbit

Isotype

IgG

Scientific Data Images for IRF7 Antibody (SC0617)

Western Blot: IRF7 Antibody (SC0617) [NBP2-67634]

Western Blot: IRF7 Antibody (SC0617) [NBP2-67634]

Western Blot: IRF7 Antibody (SC0617) [NBP2-67634] - Analysis of IRF7 on different lysates with Rabbit anti-IRF7 antibody at 1/500 dilution. Lane 1: Raji cell lysate Lane 2: 293 cell lysate Lysates/proteins at 10 ug/Lane. Predicted band size: 54 kDa Observed band size: 60 kDa Exposure time: 2 minutes; 8% SDS-PAGE gel. Proteins were transferred to a PVDF membrane and blocked with 5% NFDM/TBST for 1 hour at room temperature. The primary antibody at 1/500 dilution was used in 5% NFDM/TBST at room temperature for 2 hours. Goat Anti-Rabbit IgG - HRP Secondary Antibody at 1:300,000 dilution was used for 1 hour at room temperature.
Immunocytochemistry/ Immunofluorescence: IRF7 Antibody (SC0617) [NBP2-67634]

Immunocytochemistry/ Immunofluorescence: IRF7 Antibody (SC0617) [NBP2-67634]

Immunocytochemistry/Immunofluorescence: IRF7 Antibody (SC0617) [NBP2-67634] - Staining IRF7 in PC-12 cells (green). The nuclear counter stain is DAPI (blue). Cells were fixed in paraformaldehyde, permeabilised with 0.25% Triton X100/PBS.
Immunohistochemistry-Paraffin: IRF7 Antibody (SC0617) [NBP2-67634]

Immunohistochemistry-Paraffin: IRF7 Antibody (SC0617) [NBP2-67634]

Immunohistochemistry-Paraffin: IRF7 Antibody (SC0617) [NBP2-67634] - Analysis of paraffin-embedded mouse liver tissue using anti-IRF7 antibody. Counter stained with hematoxylin.
Flow Cytometry: IRF7 Antibody (SC0617) [NBP2-67634]

Flow Cytometry: IRF7 Antibody (SC0617) [NBP2-67634]

Flow Cytometry: IRF7 Antibody (SC0617) [NBP2-67634] - Analysis of Jurkat cells with IRF7 antibody at 1/50 dilution (red) compared with an unlabelled control (cells without incubation with primary antibody; black). Alexa Fluor 488-conjugated goat anti rabbit IgG was used as the secondary antibody
Western Blot: IRF7 Antibody (SC0617) [NBP2-67634]

Western Blot: IRF7 Antibody (SC0617) [NBP2-67634]

Western Blot: IRF7 Antibody (SC0617) [NBP2-67634] - Analysis of IRF7 on hybrid fish (crucian-carp) brain tissue lysates. Proteins were transferred to a PVDF membrane and blocked with 5% BSA in PBS for 1 hour at room temperature. The primary antibody (1/500) was used in 5% BSA at room temperature for 2 hours. Goat Anti-Rabbit IgG - HRP Secondary Antibody at 1:5,000 dilution was used for 1 hour at room temperature.
Immunocytochemistry/ Immunofluorescence: IRF7 Antibody (SC0617) [NBP2-67634]

Immunocytochemistry/ Immunofluorescence: IRF7 Antibody (SC0617) [NBP2-67634]

Immunocytochemistry/Immunofluorescence: IRF7 Antibody (SC0617) [NBP2-67634] - Staining IRF7 in 293 cells (green). The nuclear counter stain is DAPI (blue). Cells were fixed in paraformaldehyde, permeabilised with 0.25% Triton X100/PBS.
Immunocytochemistry/ Immunofluorescence: IRF7 Antibody (SC0617) [NBP2-67634]

Immunocytochemistry/ Immunofluorescence: IRF7 Antibody (SC0617) [NBP2-67634]

Immunocytochemistry/Immunofluorescence: IRF7 Antibody (SC0617) [NBP2-67634] - Staining IRF7 in HepG2 cells (green). The nuclear counter stain is DAPI (blue). Cells were fixed in paraformaldehyde, permeabilised with 0.25% Triton X100/PBS.
Immunohistochemistry-Paraffin: IRF7 Antibody (SC0617) [NBP2-67634]

Immunohistochemistry-Paraffin: IRF7 Antibody (SC0617) [NBP2-67634]

Immunohistochemistry-Paraffin: IRF7 Antibody (SC0617) [NBP2-67634] - Analysis of paraffin-embedded human kidney tissue using anti-IRF7 antibody. Counter stained with hematoxylin.
Immunohistochemistry-Paraffin: IRF7 Antibody (SC0617) [NBP2-67634]

Immunohistochemistry-Paraffin: IRF7 Antibody (SC0617) [NBP2-67634]

Immunohistochemistry-Paraffin: IRF7 Antibody (SC0617) [NBP2-67634] - Analysis of paraffin-embedded human spleen tissue using anti-IRF7 antibody. Counter stained with hematoxylin.
Immunohistochemistry-Paraffin: IRF7 Antibody (SC0617) [NBP2-67634]

Immunohistochemistry-Paraffin: IRF7 Antibody (SC0617) [NBP2-67634]

Immunohistochemistry-Paraffin: IRF7 Antibody (SC0617) [NBP2-67634] - Analysis of paraffin-embedded human tonsil tissue using anti-IRF7 antibody. Counter stained with hematoxylin.
Immunohistochemistry-Paraffin: IRF7 Antibody (SC0617) [NBP2-67634]

Immunohistochemistry-Paraffin: IRF7 Antibody (SC0617) [NBP2-67634]

Immunohistochemistry-Paraffin: IRF7 Antibody (SC0617) [NBP2-67634] - Analysis of paraffin-embedded mouse heart tissue using anti-IRF7 antibody. Counter stained with hematoxylin.
Immunohistochemistry-Paraffin: IRF7 Antibody (SC0617) [NBP2-67634]

Immunohistochemistry-Paraffin: IRF7 Antibody (SC0617) [NBP2-67634]

Immunohistochemistry-Paraffin: IRF7 Antibody (SC0617) [NBP2-67634] - Analysis of paraffin-embedded mouse kidney tissue using anti-IRF7 antibody. Counter stained with hematoxylin.

Applications for IRF7 Antibody (SC0617)

Application
Recommended Usage

Flow Cytometry

1:50-1:100

Immunocytochemistry/ Immunofluorescence

1:100-1:500

Immunohistochemistry-Paraffin

1:50-1:200

Western Blot

1:1000-1:2000
Application Notes
IP: Use at an assay dependent concentration.

Flow Cytometry Panel Builder

Bio-Techne Knows Flow Cytometry

Save time and reduce costly mistakes by quickly finding compatible reagents using the Panel Builder Tool.

Advanced Features

  • Spectra Viewer - Custom analysis of spectra from multiple fluorochromes
  • Spillover Popups - Visualize the spectra of individual fluorochromes
  • Antigen Density Selector - Match fluorochrome brightness with antigen density
Build Your Panel Now
Flow Cytometry

Formulation, Preparation, and Storage

Purification

Protein A purified

Formulation

TBS (pH7.4), 0.05% BSA, 40% Glycerol

Preservative

0.05% Sodium Azide

Concentration

1 mg/ml

Shipping

The product is shipped with polar packs. Upon receipt, store it immediately at the temperature recommended below.

Stability & Storage

Store at 4C short term. Aliquot and store at -20C long term. Avoid freeze-thaw cycles.

Background: IRF7

IRF7 encodes interferon regulatory factor 7, a member of the interferon regulatory transcription factor (IRF) family. IRF7 has been shown to play a role in the transcriptional activation of virus-inducible cellular genes, including interferon beta chain genes. Inducible expression of IRF7 is largely restricted to lymphoid tissue. Multiple IRF7 transcript variants have been identified, although the functional consequences of these have not yet been established.

Alternate Names

interferon regulatory factor 7, interferon regulatory factor-7H, IRF-7, IRF7A, IRF-7H

Gene Symbol

IRF7

Additional IRF7 Products

Product Documents for IRF7 Antibody (SC0617)

Certificate of Analysis

To download a Certificate of Analysis, please enter a lot or batch number in the search box below.

Download SDS

Product Specific Notices for IRF7 Antibody (SC0617)

This product is for research use only and is not approved for use in humans or in clinical diagnosis. Primary Antibodies are guaranteed for 1 year from date of receipt.

Citations for IRF7 Antibody (SC0617)

Customer Reviews for IRF7 Antibody (SC0617)

There are currently no reviews for this product. Be the first to review IRF7 Antibody (SC0617) and earn rewards!

Have you used IRF7 Antibody (SC0617)?

Submit a review and receive an Amazon gift card!

$25/€18/£15/$25CAN/¥2500 Yen for a review with an image

$10/€7/£6/$10CAN/¥1110 Yen for a review without an image

Submit a review
Amazon Gift Card

Protocols

Find general support by application which include: protocols, troubleshooting, illustrated assays, videos and webinars.

FAQs

No product specific FAQs exist for this product.

View all FAQs for Antibodies