Irisin/FNDC5 Antibody - BSA Free

Novus Biologicals | Catalog # NBP2-14024

Novus Biologicals
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Key Product Details

Species Reactivity

Validated:

Human, Mouse

Cited:

Human

Predicted:

Rat (100%). Backed by our 100% Guarantee.

Applications

Validated:

Immunohistochemistry, Immunohistochemistry-Paraffin, Immunofluorescence

Cited:

Immunohistochemistry, Immunohistochemistry-Paraffin, Immunofluorescence, Immunocytochemistry/ Immunofluorescence, IF/IHC

Label

Unconjugated

Antibody Source

Polyclonal Rabbit IgG

Format

BSA Free
View all Irisin/FNDC5 Primary Antibodies »

Product Specifications

Immunogen

This antibody was developed against a recombinant protein corresponding to the amino acids: EDTEYIVHVQAISIQGQSPASEPVLFKTPREAEKMASKNKDEVTMKEMGRNQQLR

Reactivity Notes

Mouse reactivity reported from a verified customer review.

Clonality

Polyclonal

Host

Rabbit

Isotype

IgG

Scientific Data Images for Irisin/FNDC5 Antibody - BSA Free

Immunohistochemistry-Paraffin: Irisin/FNDC5 Antibody [NBP2-14024]

Immunohistochemistry-Paraffin: Irisin/FNDC5 Antibody [NBP2-14024]

Immunohistochemistry-Paraffin: Irisin/FNDC5 Antibody [NBP2-14024] - Specific positive staining can be observed in a subpopulation of mouse gastric mucosa cells. Green is FNDC5. Image from verified customer review.
Immunohistochemistry-Paraffin: Irisin/FNDC5 Antibody [NBP2-14024]

Immunohistochemistry-Paraffin: Irisin/FNDC5 Antibody [NBP2-14024]

Immunohistochemistry-Paraffin: Irisin/FNDC5 Antibody [NBP2-14024] - Staining of human skeletal muscle shows granular cytoplasmic positivity in myocytes.
Irisin/FNDC5 Antibody

Immunohistochemistry: Irisin/FNDC5 Antibody [NBP2-14024] -

Immunohistochemistry: Irisin/FNDC5 Antibody [NBP2-14024] - Positive immunohistochemical (IHC) reactions (brown color) indicating strong irisin expression in the apical parts of cancer cells & vesicles; magnification x400. Image collected & cropped by CiteAb from the following publication (https://pubmed.ncbi.nlm.nih.gov/35408891), licensed under a CC-BY license. Not internally tested by Novus Biologicals.
Irisin/FNDC5 Antibody

Immunohistochemistry: Irisin/FNDC5 Antibody [NBP2-14024] -

Immunohistochemistry: Irisin/FNDC5 Antibody [NBP2-14024] - Comparison of irisin expression using immunohistochemistry (IHC; positive reactions—brown cell cytoplasm) in control tissue (A) & at different grades of malignancy (G) of breast cancer (BC; (B)—G1; (C)—G2, in apical parts of cells; (D)—G3) with Ki-67 (E) & PGC-1 alpha (F); magnification x200. Image collected & cropped by CiteAb from the following publication (https://pubmed.ncbi.nlm.nih.gov/35408891), licensed under a CC-BY license. Not internally tested by Novus Biologicals.

Applications for Irisin/FNDC5 Antibody - BSA Free

Application
Recommended Usage

Immunofluorescence

Reported in scientific literature (PMID:31614634).

Immunohistochemistry

1:20 - 1:50

Immunohistochemistry-Paraffin

1:20-1:50
Application Notes
For IHC-Paraffin, HIER pH 6 retrieval is recommended.

Reviewed Applications

Read 1 review rated 5 using NBP2-14024 in the following applications:

Formulation, Preparation, and Storage

Purification

Affinity purified

Formulation

PBS (pH 7.2) and 40% Glycerol

Format

BSA Free

Preservative

0.02% Sodium Azide

Concentration

Concentrations vary lot to lot. See vial label for concentration. If unlisted please contact technical services.

Shipping

The product is shipped with polar packs. Upon receipt, store it immediately at the temperature recommended below.

Stability & Storage

Store at 4C short term. Aliquot and store at -20C long term. Avoid freeze-thaw cycles.

Background: Irisin/FNDC5

Irisin, also known as FNDC5, is a peptide hormone that is upregulated in skeletal muscle and subcutaneous adipose tissue during exercise. It induces expression of the mitochondrial proteins PPAR-gamma coactivator 1 alpha (PGC1a) and uncoupling protein-1 (UCP1). It contributes to the regulation of diabetes and obesity by inducing the transition of white adipose tissue into more metabolically active beige adipose tissue. Irisin also regulates neuronal cell differentiation, neurite outgrowth, and osteoblast differentiation.

Long Name

Fibronectin Type III Domain Containing 5

Alternate Names

FNDC5, FRCP2

Gene Symbol

FNDC5

Additional Irisin/FNDC5 Products

Product Documents for Irisin/FNDC5 Antibody - BSA Free

Certificate of Analysis

To download a Certificate of Analysis, please enter a lot or batch number in the search box below.

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Product Specific Notices for Irisin/FNDC5 Antibody - BSA Free

This product is for research use only and is not approved for use in humans or in clinical diagnosis. Primary Antibodies are guaranteed for 1 year from date of receipt.

Related Research Areas

Diabetes
Obesity

Citations for Irisin/FNDC5 Antibody - BSA Free

Customer Reviews for Irisin/FNDC5 Antibody - BSA Free (1)

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Customer Images

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  • Irisin/FNDC5 Antibody
    Name: Anonymous
    Application: Immunohistochemistry-Paraffin
    Sample Tested: Stomach tissue
    Species: Mouse
    Verified Customer | Posted 01/23/2019
    Specific positive staining can be observed in a subpopulation of mouse gastric mucosa cells. Green is FNDC5. This antibody is very good.
    Irisin/FNDC5 Antibody - BSA Free NBP2-14024

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