IRP2 Antibody - BSA Free

Novus Biologicals | Catalog # NBP3-03393

Novus Biologicals
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Key Product Details

Species Reactivity

Human, Mouse, Rat

Applications

Western Blot, ELISA, Immunocytochemistry/ Immunofluorescence

Label

Unconjugated

Antibody Source

Polyclonal Rabbit IgG

Format

BSA Free
View all IRP2 Primary Antibodies »

Product Specifications

Immunogen

Recombinant fusion protein containing a sequence corresponding to amino acids 760-963 of human IRP2 (NP_004127.1). SIARNSAAAKYLTNRGLTPREFNSYGARRGNDAVMTRGTFANIKLFNKFIGKPAPKTIHFPSGQTLDVFEAAELYQKEGIPLIILAGKKYGSGNSRDWAAKGPYLLGVKAVLAESYEKIHKDHLIGIGIAPLQFLPGENADSLGLSGRETFSLTFPEELSPGITLNIQTSTGKVFSVIASFEDDVEITLYKHGGLLNFVARKFS

Clonality

Polyclonal

Host

Rabbit

Isotype

IgG

Scientific Data Images for IRP2 Antibody - BSA Free

Western Blot: IRP2 AntibodyAzide and BSA Free [NBP3-03393]

Western Blot: IRP2 AntibodyAzide and BSA Free [NBP3-03393]

Western Blot: IRP2 Antibody [NBP3-03393] - Western blot analysis of extracts of various cell lines, using IRP2 antibody (NBP3-03393) at 1:1000 dilution. Secondary antibody: HRP Goat Anti-Rabbit IgG (H+L) at 1:10000 dilution. Lysates/proteins: 25ug per lane. Blocking buffer: 3% nonfat dry milk in TBST. Detection: ECL Basic Kit. Exposure time: 3s.
IRP2 Antibody - Azide and BSA Free

Immunocytochemistry/ Immunofluorescence: IRP2 Antibody - Azide and BSA Free [IRP2] -

Immunocytochemistry/ Immunofluorescence: IRP2 Antibody - Azide and BSA Free [IRP2] - Immunofluorescence analysis of A549 cells using IRP2 Rabbit pAb. Secondary antibody: Cy3-conjugated Goat anti-Rabbit IgG (H+L) at 1:500 dilution. Blue: DAPI for nuclear staining.

Applications for IRP2 Antibody - BSA Free

Application
Recommended Usage

Immunocytochemistry/ Immunofluorescence

1:20-1:50

Western Blot

1:500 - 1:1000

Formulation, Preparation, and Storage

Purification

Affinity purified

Formulation

PBS (pH 7.3), 50% glycerol

Format

BSA Free

Preservative

0.02% Sodium Azide

Concentration

Please see the vial label for concentration. If unlisted please contact technical services.

Shipping

The product is shipped with polar packs. Upon receipt, store it immediately at the temperature recommended below.

Stability & Storage

Store at -20C. Avoid freeze-thaw cycles.

Background: IRP2

Iron regulatory protein 2 (IRP2) is a central post-transcriptional regulator of cellular and systemic iron metabolism. It is a cytosolic RNA-binding protein that binds to stem-loop structures. IRP2 is regulated by iron-mediated proteolysis. IRP2 also plays a role during hypoxia, as iron and oxygen are closely related.

Alternate Names

ACO3, EC 4.2.1.3, FLJ23381, IRE-BP 2, Iron regulatory protein 2, iron-responsive element binding protein 2, iron-responsive element-binding protein 2, IRP2IRP2AD

Gene Symbol

IREB2

Additional IRP2 Products

Product Documents for IRP2 Antibody - BSA Free

Certificate of Analysis

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Product Specific Notices for IRP2 Antibody - BSA Free

This product is for research use only and is not approved for use in humans or in clinical diagnosis. Primary Antibodies are guaranteed for 1 year from date of receipt.

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Protocols

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FAQs

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