ISG15/UCRP Antibody (239) - Azide and BSA Free
Novus Biologicals | Catalog # NBP2-90146
Recombinant Monoclonal Antibody
![Western Blot: ISG15/UCRP Antibody (239) [NBP2-90146]](https://resources.rndsystems.com/images/products/ISG15-UCRP-Antibody-239-Western-Blot-NBP2-90146-img0002.jpg "Western Blot: ISG15/UCRP Antibody (239) [NBP2-90146]")
Key Product Details
Species Reactivity
Human
Applications
Western Blot, ELISA, Immunocytochemistry/ Immunofluorescence
Label
Unconjugated
Antibody Source
Recombinant Monoclonal Rabbit IgG Clone # 239 expressed in HEK293
Format
Azide and BSA Free
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Product Specifications
Immunogen
This antibody was obtained from a rabbit immunized with purified, recombinant Human ISG15/UCRP (Accession#: AAH09507.1; Met1-Ser165).
Clonality
Monoclonal
Host
Rabbit
Isotype
IgG
Description
This antibody can be stored at 2C-8C for one month without detectable loss of activity. Antibody products are stable for twelve months from date of receipt when stored at -20C to -80C. Avoid repeated freeze-thaw cycles.
Scientific Data Images for ISG15/UCRP Antibody (239) - Azide and BSA Free
Western Blot: ISG15/UCRP Antibody (239) [NBP2-90146]
Western Blot: ISG15/UCRP Antibody (239) [NBP2-90146] - Lane A: Hela Whole Cell LysateLane B: 293 Whole Cell LysateLane C: Daudi Whole Cell LysateLysates/proteins at 30 ug per lane.SecondaryGoat Anti-Rabbit IgG H&L (Dylight800) at 1/10000 dilution.Developed using the Odyssey technique. Performed under reducing conditions.Predicted band size:18 kDaObserved band size:15 kDa
Immunocytochemistry/Immunofluorescence: ISG15/UCRP Antibody (239) [NBP2-90146] -
Immunocytochemistry/Immunofluorescence: ISG15/UCRP Antibody (239) [NBP2-90146] - Immunofluorescence staining of Human ProISG15 in Hela cells. Cells were fixed with 4% PFA, permeabilzed with 0.3% Triton X-100 in PBS, blocked with 10% serum, and incubated with NBP2-90146 antibody (1:60) at 37? 1 hour. Then cells were stained with the Alexa Fluo(R) 488-conjugated Goat Anti-rabbit IgG secondary antibody (green). Positive staining was localized to cytoplasm.Applications for ISG15/UCRP Antibody (239) - Azide and BSA Free
Application
Recommended Usage
ELISA
1:5000-1:10000
Immunocytochemistry/ Immunofluorescence
1:20-1:100
Western Blot
1:2000-1:10000
Formulation, Preparation, and Storage
Purification
Protein A purified
Formulation
0.2 um filtered solution in PBS
Format
Azide and BSA Free
Preservative
No Preservative
Concentration
Please see the vial label for concentration. If unlisted please contact technical services.
Shipping
The product is shipped with polar packs. Upon receipt, store it immediately at the temperature recommended below.
Stability & Storage
Store at 4C short term. Aliquot and store at -20C long term. Avoid freeze-thaw cycles.
Background: ISG15/UCRP
Long Name
ISG15 Ubiquitin-like Modifier
Alternate Names
G1P2, IFI15, IP17, UCRP
Gene Symbol
ISG15
Additional ISG15/UCRP Products
Product Documents for ISG15/UCRP Antibody (239) - Azide and BSA Free
Certificate of Analysis
To download a Certificate of Analysis, please enter a lot or batch number in the search box below.
Product Specific Notices for ISG15/UCRP Antibody (239) - Azide and BSA Free
This product is for research use only and is not approved for use in humans or in clinical diagnosis. Primary Antibodies are guaranteed for 1 year from date of receipt.
Related Research Areas
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Protocols
Find general support by application which include: protocols, troubleshooting, illustrated assays, videos and webinars.
- Appropriate Fixation of IHC/ICC Samples
- Cellular Response to Hypoxia Protocols
- ClariTSA™ Fluorophore Kits
- Detection & Visualization of Antibody Binding
- ELISA Sample Preparation & Collection Guide
- ELISA Troubleshooting Guide
- How to Run an R&D Systems DuoSet ELISA
- How to Run an R&D Systems Quantikine ELISA
- How to Run an R&D Systems Quantikine™ QuicKit™ ELISA
- ICC Cell Smear Protocol for Suspension Cells
- ICC Immunocytochemistry Protocol Videos
- ICC for Adherent Cells
- Immunocytochemistry (ICC) Protocol
- Immunocytochemistry Troubleshooting
- Immunofluorescence of Organoids Embedded in Cultrex Basement Membrane Extract
- Immunohistochemistry (IHC) and Immunocytochemistry (ICC) Protocols
- Preparing Samples for IHC/ICC Experiments
- Preventing Non-Specific Staining (Non-Specific Binding)
- Primary Antibody Selection & Optimization
- Protocol for VisUCyte™ HRP Polymer Detection Reagent
- Protocol for the Fluorescent ICC Staining of Cell Smears - Graphic
- Protocol for the Fluorescent ICC Staining of Cultured Cells on Coverslips - Graphic
- Protocol for the Preparation and Fluorescent ICC Staining of Cells on Coverslips
- Protocol for the Preparation and Fluorescent ICC Staining of Non-adherent Cells
- Protocol for the Preparation and Fluorescent ICC Staining of Stem Cells on Coverslips
- Protocol for the Preparation of a Cell Smear for Non-adherent Cell ICC - Graphic
- Quantikine HS ELISA Kit Assay Principle, Alkaline Phosphatase
- Quantikine HS ELISA Kit Principle, Streptavidin-HRP Polymer
- R&D Systems Quality Control Western Blot Protocol
- Sandwich ELISA (Colorimetric) – Biotin/Streptavidin Detection Protocol
- Sandwich ELISA (Colorimetric) – Direct Detection Protocol
- TUNEL and Active Caspase-3 Detection by IHC/ICC Protocol
- The Importance of IHC/ICC Controls
- Troubleshooting Guide: ELISA
- Troubleshooting Guide: Western Blot Figures
- Western Blot Conditions
- Western Blot Protocol
- Western Blot Protocol for Cell Lysates
- Western Blot Troubleshooting
- Western Blot Troubleshooting Guide
- View all Protocols, Troubleshooting, Illustrated assays and Webinars
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