ISG15/UCRP Antibody - BSA Free

Novus Biologicals | Catalog # NB600-891

Novus Biologicals
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Key Product Details

Species Reactivity

Human

Applications

Immunohistochemistry, Immunohistochemistry-Paraffin, Western Blot, ELISA

Label

Unconjugated

Antibody Source

Polyclonal Rabbit IgG

Format

BSA Free
View all ISG15/UCRP Primary Antibodies »

Product Specifications

Immunogen

This purified ISG15/UCRP Antibody was prepared from rabbit serum after repeated immunizations with recombinant human ISG15/UCRP/UCRP protein. (Uniprot: P05161)

Localization

Cytoplasmic and Secreted

Clonality

Polyclonal

Host

Rabbit

Isotype

IgG

Description

This product is an IgG fraction antibody purified from monospecific antiserum by a multi-step process which includes delipidation, salt fractionation and ion exchange chromatography followed by extensive dialysis against the buffer stated above. Assay by immunoelectrophoresis resulted in a single precipitin arc against anti-Rabbit Serum.

Store vial at 4C prior to restoration. For extended storage aliquot contents and freeze at -20C or below. Avoid cycles of freezing and thawing. Centrifuge product if not completely clear after standing at room temperature. This product is stable for several weeks at 4C as an undiluted liquid. Dilute only prior to immediate use.

Scientific Data Images for ISG15/UCRP Antibody - BSA Free

Western Blot: ISG15/UCRP Antibody [NB600-891]

Western Blot: ISG15/UCRP Antibody [NB600-891]

Western Blot: ISG15/UCRP Antibody [NB600-891] - Immunoblot of hISG15 fusion protein. Anti-hISG15 antibody, generated by immunization with recombinant human ISG15, was tested by immunoblot against a hISG15-GFP fusion protein produced in E.coli cell lysate soluble fraction. Dilution of the antibody between 1:200 and 1:1,000 showed strong reactivity specifically with hISG15 and ISG15 coupled proteins. Free hISG15 is indicated by the arrowhead. In this blot the antibody was used at a 1:200 dilution incubated overnight at 4C in 5% non-fat dry milk in TTBS. Detection occurred using a 1:2000 dilution of HRP labeled Donkey anti-Rabbit IgG for 1h at room temperature. A chemiluminescence system was used for signal detection.
N/A: ISG15/UCRP Antibody [NB600-891]

Applications for ISG15/UCRP Antibody - BSA Free

Application
Recommended Usage

ELISA

1:2000-1:10000

Immunohistochemistry

1:10-1:500

Immunohistochemistry-Paraffin

1:10-1:500

Western Blot

1:200-1:1000
Application Notes
This purified polyclonal antibody reacts with human ISG15 by western blot and ELISA. Although not tested, this antibody is likely functional in immunohistochemistry and immunoprecipitation. This antibody using the specified conditions may recognize other prominent intrinsic bands (UBLs or conjugates), especially at lower dilutions. An 18.5 kDa band corresponding to human ISG15 is detected. IFNalpha or IFNbeta stimulated HeLa cell lysates can be used as a positive control.

Formulation, Preparation, and Storage

Purification

Multi-step

Reconstitution

For the 500 ug size, we recommend reconstituting with 100 ul deionized water. The 0.1 mg size is provided in liquid format (0.02 ml).

Formulation

Lyophilized from 0.02 M Potassium Phosphate, 0.15 M Sodium Chloride, pH 7.2

Format

BSA Free

Preservative

0.01% Sodium Azide

Concentration

LYOPH mg/ml

Shipping

The product is shipped with polar packs. Upon receipt, store it immediately at the temperature recommended below.

Stability & Storage

Store lyophilized antibody at 4C. Aliquot reconstituted liquid and store at -20C. Avoid freeze-thaw cycles.

Calculators

The reconstitution calculator allows you to quickly calculate the volume of a reagent to reconstitute your vial. Simply enter the mass of reagent and the target concentration and the calculator will determine the rest.

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Background: ISG15/UCRP

ISG15 a 15kDa homologue shows no amino acid sequence homology to cytokines and is synthesized as a precursor that is activated through processing by a thiol protease. ISG15 is secreted by monocytes and lymphocytes. Synthesis is induced in response to IFN alpha or IFN beta, but not IFN gamma. ISG15 expression is induced also by overexpression of some interferon regulatory factors that have been shown to play a role in the transcriptional regulation of IFN genes. ISG15 is secreted also by cell lines of monocyte (U937 cell line), T-lymphocyte, B-lymphocyte (DAUDI cells), human fibroblasts, and epithelial origins. The induction of terminal differentiation in human melanoma cells is associated, among other things, with alterations in the expression of ISG15. Intracellularly ISG15 has been shown to function as a ubiquitin homologue. It is known also as UCRP (ubiquitin cross-reactive protein). Serpin 2a (spi2a), a member of the serine protease inhibitor (serpin) protein family that is highly induced in macrophages during bacillus Calmette-Guerin infection has been shown to bind ISG15. ISG15 has been shown to modulate immune cell function. It possesses activities of cytokines and induces production of IFN gamma. It enhances proliferation and functions of natural killer and LAK cells.

Long Name

ISG15 Ubiquitin-like Modifier

Alternate Names

G1P2, IFI15, IP17, UCRP

Entrez Gene IDs

9636 (Human)

Gene Symbol

ISG15

UniProt

P05161 (Human)

Additional ISG15/UCRP Products

Product Documents for ISG15/UCRP Antibody - BSA Free

Certificate of Analysis

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Product Specific Notices for ISG15/UCRP Antibody - BSA Free

This product is for research use only and is not approved for use in humans or in clinical diagnosis. Primary Antibodies are guaranteed for 1 year from date of receipt.

Citations for ISG15/UCRP Antibody - BSA Free

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Protocols

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