Isocitrate Dehydrogenase 1/IDH1 Antibody (1521) - Azide and BSA Free

Novus Biologicals | Catalog # NBP2-43692

Novus Biologicals
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Key Product Details

Validated by

Knockout/Knockdown

Species Reactivity

Validated:

Human, Mouse, Rat

Predicted:

Bovine (95%), Rhesus Macaque (100%), Sheep (95%). Backed by our 100% Guarantee.

Applications

Knockout Validated, Western Blot, Immunocytochemistry/ Immunofluorescence, Immunoprecipitation, Knockdown Validated

Label

Unconjugated

Antibody Source

Monoclonal Mouse IgG2B Clone # 1521

Format

Azide and BSA Free
View all Isocitrate Dehydrogenase 1/IDH1 Primary Antibodies »

Product Specifications

Immunogen

Recombinant protein encompassing a sequence within the center region of human Isocitrate Dehydrogenase 1/IDH1. The exact sequence is proprietary.

Reactivity Notes

Zebrafish (85%), Xenopus laevis (88%).

Clonality

Monoclonal

Host

Mouse

Isotype

IgG2B

Theoretical MW

47 kDa.
Disclaimer note: The observed molecular weight of the protein may vary from the listed predicted molecular weight due to post translational modifications, post translation cleavages, relative charges, and other experimental factors.

Scientific Data Images for Isocitrate Dehydrogenase 1/IDH1 Antibody (1521) - Azide and BSA Free

Immunocytochemistry/ Immunofluorescence: Isocitrate Dehydrogenase 1/IDH1 Antibody (1521) [NBP2-43692]

Immunocytochemistry/ Immunofluorescence: Isocitrate Dehydrogenase 1/IDH1 Antibody (1521) [NBP2-43692]

Immunocytochemistry/Immunofluorescence: Isocitrate Dehydrogenase 1/IDH1 Antibody (1521) [NBP2-43692] - HepG2 cells were fixed in 4% paraformaldehyde at RT for 15 min. Green: IDH1 protein stained by IDH1 antibody [1521] diluted at 1:500. Blue: Hoechst 33342 staining.
Western Blot: Isocitrate Dehydrogenase 1/IDH1 Antibody (1521) [NBP2-43692]

Western Blot: Isocitrate Dehydrogenase 1/IDH1 Antibody (1521) [NBP2-43692]

Western Blot: Isocitrate Dehydrogenase 1/IDH1 Antibody (1521) [NBP2-43692] - Analysis of A. 30 ug 293T whole cell lysate/extract B. 30 ug A431 whole cell lysate/extract C. 30 ug HeLa whole cell lysate/extract D. 30 ug HepG2 whole cell lysate/extract 10 % SDS-PAGE IDH1 antibody [1521] dilution: 1:1000
Western Blot: Isocitrate Dehydrogenase 1/IDH1 Antibody (1521) [NBP2-43692]

Western Blot: Isocitrate Dehydrogenase 1/IDH1 Antibody (1521) [NBP2-43692]

Western Blot: Isocitrate Dehydrogenase 1/IDH1 Antibody (1521) [NBP2-43692] - Analysis of A. 30 ug Neuro2A whole cell lysate/extract B. 30 ug GL261 whole cell lysate/extract C. 30 ug C8D30 whole cell lysate/extract D. 30 ug NIH-3T3 whole cell lysate/extract E. 30 ug Raw264.7 whole cell lysate/extract F. 30 ug C2C12 whole cell lysate/extract 10 % SDS-PAGE IDH1 antibody [1521] dilution: 1:1000
Western Blot: Isocitrate Dehydrogenase 1/IDH1 Antibody (1521) [NBP2-43692]

Western Blot: Isocitrate Dehydrogenase 1/IDH1 Antibody (1521) [NBP2-43692]

Western Blot: Isocitrate Dehydrogenase 1/IDH1 Antibody (1521) [NBP2-43692] - Analysis of A. 30 ug PC-12 whole cell lysate/extract B. 30 ug Rat-2 whole cell lysate/extract 10 % SDS-PAGE IDH1 antibody [1521] dilution: 1:1000
Immunoprecipitation: Isocitrate Dehydrogenase 1/IDH1 Antibody (1521) [NBP2-43692]

Immunoprecipitation: Isocitrate Dehydrogenase 1/IDH1 Antibody (1521) [NBP2-43692]

Immunoprecipitation: Isocitrate Dehydrogenase 1/IDH1 Antibody (1521) [NBP2-43692] - Analysis of HepG2 whole cell lysate/extract A : 30 ug whole cell lysate/extract of IDH1 protein expressing HepG2 cells B : Control with 2.5 ug of pre-immune mouse IgG C : Immunoprecipitation of IDH-1 protein by 2.5 ug of IDH-1 antibody 10% SDS-PAGE The immunoprecipitated IDH-1 protein was detected by IDH-1 antibody diluted at 1 : 1000. EasyBlot anti-mouse IgG (HRP) was used as a secondary reagent.
Isocitrate Dehydrogenase 1/IDH1 Antibody (1521) - Azide and BSA Free

Western Blot: Isocitrate Dehydrogenase 1/IDH1 Antibody (1521) [NBP2-43692] -

Non-transfected (-) and transfected (+) HepG2 whole cell extracts (30 ug) were separated by 10% SDS-PAGE, and the membrane was blotted with IDH1 antibody [GT1521] (NBP2-43692) diluted at 1:4000. The HRP-conjugated anti-mouse IgG antibody was used to detect the primary antibody.
Isocitrate Dehydrogenase 1/IDH1 Antibody (1521) - Azide and BSA Free

Western Blot: Isocitrate Dehydrogenase 1/IDH1 Antibody (1521) [NBP2-43692] -

Non-transfected (-) and transfected (+) 293T whole cell extracts (30 ug) were separated by 10% SDS-PAGE, and the membrane was blotted with IDH1 antibody [GT1521] (NBP2-43692) diluted at 1:5000. The HRP-conjugated anti-mouse IgG antibody was used to detect the primary antibody.
Isocitrate Dehydrogenase 1/IDH1 Antibody (1521) - Azide and BSA Free

Western Blot: Isocitrate Dehydrogenase 1/IDH1 Antibody (1521) [NBP2-43692] -

Wild-type (WT) and IDH1 knockout (KO) HeLa cell extracts (30 ug) were separated by 10% SDS-PAGE, and the membrane was blotted with IDH1 antibody [GT1521] (NBP2-43692) diluted at 1:500. The HRP-conjugated anti-mouse IgG antibody was used to detect the primary antibody.

Applications for Isocitrate Dehydrogenase 1/IDH1 Antibody (1521) - Azide and BSA Free

Application
Recommended Usage

Immunocytochemistry/ Immunofluorescence

1:100-1:1000

Immunoprecipitation

1:100-1:1000

Western Blot

1:500-1:10000

Formulation, Preparation, and Storage

Purification

Protein G purified

Formulation

PBS

Format

Azide and BSA Free

Preservative

No Preservative

Concentration

Concentrations vary lot to lot. See vial label for concentration. If unlisted please contact technical services.

Shipping

The product is shipped with polar packs. Upon receipt, store it immediately at the temperature recommended below.

Stability & Storage

Aliquot and store at -20C or -80C. Avoid freeze-thaw cycles.

Background: Isocitrate Dehydrogenase 1/IDH1

Isocitrate dehydrogenases catalyze the oxidative decarboxylation of isocitrate to 2-oxoglutarate. These enzymes belong to two distinct subclasses, one of which utilizes NAD(+) as the electron acceptor and the other NADP(+). Five isocitrate dehydrogenases have been reported: three NAD(+)-dependent isocitrate dehydrogenases, which localize to the mitochondrial matrix, and two NADP(+)-dependent isocitrate dehydrogenases, one of which is mitochondrial and the other predominantly cytosolic. Each NADP(+)-dependent isozyme is a homodimer. The protein encoded by this gene is the NADP(+)-dependent isocitrate dehydrogenase found in the cytoplasm and peroxisomes. It contains the PTS-1 peroxisomal targeting signal sequence. The presence of this enzyme in peroxisomes suggests roles in the regeneration of NADPH for intraperoxisomal reductions, such as the conversion of 2, 4-dienoyl-CoAs to 3-enoyl-CoAs, as well as in peroxisomal reactions that consume 2-oxoglutarate, namely the alpha-hydroxylation of phytanic acid. The cytoplasmic enzyme serves a significant role in cytoplasmic NADPH production. [provided by RefSeq]

Alternate Names

IDCD, IDH1, IDP, IDPC, PICD

Gene Symbol

IDH1

Additional Isocitrate Dehydrogenase 1/IDH1 Products

Product Documents for Isocitrate Dehydrogenase 1/IDH1 Antibody (1521) - Azide and BSA Free

Certificate of Analysis

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Product Specific Notices for Isocitrate Dehydrogenase 1/IDH1 Antibody (1521) - Azide and BSA Free

This product is for research use only and is not approved for use in humans or in clinical diagnosis. Primary Antibodies are guaranteed for 1 year from date of receipt.

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Protocols

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