Isocitrate Dehydrogenase 1/IDH1 Antibody (7G8A1) - BSA Free

Novus Biologicals | Catalog # NBP2-61795

Novus Biologicals
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Key Product Details

Species Reactivity

Human, Mouse, Monkey

Applications

Western Blot, ELISA, Flow Cytometry, Immunocytochemistry/ Immunofluorescence

Label

Unconjugated

Antibody Source

Monoclonal Mouse IgG1 Clone # 7G8A1

Format

BSA Free
View all Isocitrate Dehydrogenase 1/IDH1 Primary Antibodies »

Product Specifications

Immunogen

Purified recombinant fragment of human Isocitrate Dehydrogenase 1/IDH1 (AA: 156-298) expressed in E. Coli.

Clonality

Monoclonal

Host

Mouse

Isotype

IgG1

Scientific Data Images for Isocitrate Dehydrogenase 1/IDH1 Antibody (7G8A1) - BSA Free

Western Blot: Isocitrate Dehydrogenase 1/IDH1 Antibody (7G8A1)BSA Free [NBP2-61795]

Western Blot: Isocitrate Dehydrogenase 1/IDH1 Antibody (7G8A1)BSA Free [NBP2-61795]

Western Blot: Isocitrate Dehydrogenase 1/IDH1 Antibody (7G8A1) [NBP2-61795] - Analysis using IDH1 mAb against HEK293 (1) and IDH1 (AA: 156-298)-hIgGFc transfected HEK293 (2) cell lysate.
Immunocytochemistry/ Immunofluorescence: Isocitrate Dehydrogenase 1/IDH1 Antibody (7G8A1) - BSA Free [NBP2-61795]

Immunocytochemistry/ Immunofluorescence: Isocitrate Dehydrogenase 1/IDH1 Antibody (7G8A1) - BSA Free [NBP2-61795]

Immunocytochemistry/Immunofluorescence: Isocitrate Dehydrogenase 1/IDH1 Antibody (7G8A1) [NBP2-61795] - Analysis of Hela cells using IDH1 mouse mAb (green). Blue: DRAQ5 fluorescent DNA dye. Red: Actin filaments have been labeled with Alexa Fluor- 555 phalloidin. Goat anti-Mouse IgG (H+L) DyLight 488 secondary antibody was used.
Flow Cytometry: Isocitrate Dehydrogenase 1/IDH1 Antibody (7G8A1) - BSA Free [NBP2-61795]

Flow Cytometry: Isocitrate Dehydrogenase 1/IDH1 Antibody (7G8A1) - BSA Free [NBP2-61795]

Flow Cytometry: Isocitrate Dehydrogenase 1/IDH1 Antibody (7G8A1) [NBP2-61795] - Analysis of Hela cells using IDH1 mouse mAb (green) and negative control (red).
Western Blot: Isocitrate Dehydrogenase 1/IDH1 Antibody (7G8A1)BSA Free [NBP2-61795]

Western Blot: Isocitrate Dehydrogenase 1/IDH1 Antibody (7G8A1)BSA Free [NBP2-61795]

Western Blot: Isocitrate Dehydrogenase 1/IDH1 Antibody (7G8A1) [NBP2-61795] - Analysis using IDH1 mAb against human IDH1 (AA: 156-298) recombinant protein. (Expected MW is 41.8 kDa)
Western Blot: Isocitrate Dehydrogenase 1/IDH1 Antibody (7G8A1)BSA Free [NBP2-61795]

Western Blot: Isocitrate Dehydrogenase 1/IDH1 Antibody (7G8A1)BSA Free [NBP2-61795]

Western Blot: Isocitrate Dehydrogenase 1/IDH1 Antibody (7G8A1) [NBP2-61795] - Analysis using IDH1 mouse mAb against HepG2 (1), NIH/3T3 (2), C2C12 (3), COS7 (4), and SW480 (5) cell lysate.
ELISA: Isocitrate Dehydrogenase 1/IDH1 Antibody (7G8A1) - BSA Free [NBP2-61795]

ELISA: Isocitrate Dehydrogenase 1/IDH1 Antibody (7G8A1) - BSA Free [NBP2-61795]

ELISA: Isocitrate Dehydrogenase 1/IDH1 Antibody (7G8A1) [NBP2-61795] - Black line: Control Antigen (100 ng);Purple line: Antigen (10ng); Blue line: Antigen (50 ng); Red line:Antigen (100 ng)

Applications for Isocitrate Dehydrogenase 1/IDH1 Antibody (7G8A1) - BSA Free

Application
Recommended Usage

ELISA

1:10000

Flow Cytometry

1:200-1:400

Immunocytochemistry/ Immunofluorescence

1:50-1:250

Western Blot

1:500-1:2000

Flow Cytometry Panel Builder

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Advanced Features

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  • Antigen Density Selector - Match fluorochrome brightness with antigen density
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Flow Cytometry

Formulation, Preparation, and Storage

Purification

Protein G purified

Formulation

PBS

Format

BSA Free

Preservative

0.05% Sodium Azide

Concentration

1 mg/ml

Shipping

The product is shipped with polar packs. Upon receipt, store it immediately at the temperature recommended below.

Stability & Storage

Store at 4C short term. Aliquot and store at -20C long term. Avoid freeze-thaw cycles.

Background: Isocitrate Dehydrogenase 1/IDH1

Isocitrate dehydrogenases catalyze the oxidative decarboxylation of isocitrate to 2-oxoglutarate. These enzymes belong to two distinct subclasses, one of which utilizes NAD(+) as the electron acceptor and the other NADP(+). Five isocitrate dehydrogenases have been reported: three NAD(+)-dependent isocitrate dehydrogenases, which localize to the mitochondrial matrix, and two NADP(+)-dependent isocitrate dehydrogenases, one of which is mitochondrial and the other predominantly cytosolic. Each NADP(+)-dependent isozyme is a homodimer. The protein encoded by this gene is the NADP(+)-dependent isocitrate dehydrogenase found in the cytoplasm and peroxisomes. It contains the PTS-1 peroxisomal targeting signal sequence. The presence of this enzyme in peroxisomes suggests roles in the regeneration of NADPH for intraperoxisomal reductions, such as the conversion of 2, 4-dienoyl-CoAs to 3-enoyl-CoAs, as well as in peroxisomal reactions that consume 2-oxoglutarate, namely the alpha-hydroxylation of phytanic acid. The cytoplasmic enzyme serves a significant role in cytoplasmic NADPH production. Alternatively spliced transcript variants encoding the same protein have been found for this gene.;

Alternate Names

IDCD, IDH1, IDP, IDPC, PICD

Gene Symbol

IDH1

Additional Isocitrate Dehydrogenase 1/IDH1 Products

Product Documents for Isocitrate Dehydrogenase 1/IDH1 Antibody (7G8A1) - BSA Free

Certificate of Analysis

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Product Specific Notices for Isocitrate Dehydrogenase 1/IDH1 Antibody (7G8A1) - BSA Free

This product is for research use only and is not approved for use in humans or in clinical diagnosis. Primary Antibodies are guaranteed for 1 year from date of receipt.

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Protocols

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