Isocitrate Dehydrogenase 1/IDH1 Antibody (IDH1/1152)

Novus Biologicals | Catalog # NBP2-45156

Novus Biologicals
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Key Product Details

Species Reactivity

Human

Applications

Immunohistochemistry, Immunohistochemistry-Paraffin, Western Blot, Flow Cytometry, Immunocytochemistry/ Immunofluorescence

Label

Unconjugated

Antibody Source

Monoclonal Mouse IgG1 kappa Clone # IDH1/1152
View all Isocitrate Dehydrogenase 1/IDH1 Primary Antibodies »

Product Specifications

Immunogen

Recombinant fragment of human Isocitrate Dehydrogenase 1/IDH1 protein (around aa 281-414) (exact sequence is proprietary) (Uniprot: O75874 )

Localization

Cytoplasmic & Nuclear

Clonality

Monoclonal

Host

Mouse

Isotype

IgG1 kappa

Description

200ug/ml of antibody purified from Bioreactor Concentrate by Protein A or G. Prepared in 1 mM PBS with 0.05% BSA & 0.05% azide. Also available WITHOUT BSA & azide at 1.0 mg/ml. (NBP2-47744)

Antibody with azide - store at 2 to 8C. Antibody without azide - store at -20 to -80 C.

Scientific Data Images for Isocitrate Dehydrogenase 1/IDH1 Antibody (IDH1/1152)

Immunocytochemistry/ Immunofluorescence: Isocitrate Dehydrogenase 1/IDH1 Antibody (IDH1/1152) [NBP2-45156]

Immunocytochemistry/ Immunofluorescence: Isocitrate Dehydrogenase 1/IDH1 Antibody (IDH1/1152) [NBP2-45156]

Immunocytochemistry/Immunofluorescence: Isocitrate Dehydrogenase 1/IDH1 Antibody (IDH1/1152) [NBP2-45156] - Immunofluorescence Analysis of PFA-fixed HeLa cells labeling IDH1. Isocitrate Dehydrogenase 1/IDH1 antibody (IDH1/1152) followed by goat anti-mouse IgG-CF488 (green). Nuclei counterstained with RedDot.
Immunohistochemistry-Paraffin: Isocitrate Dehydrogenase 1/IDH1 Antibody (IDH1/1152) [NBP2-45156]

Immunohistochemistry-Paraffin: Isocitrate Dehydrogenase 1/IDH1 Antibody (IDH1/1152) [NBP2-45156]

Immunohistochemistry-Paraffin: Isocitrate Dehydrogenase 1/IDH1 Antibody (IDH1/1152) [NBP2-45156] - Formalin-fixed, paraffin-embedded human Colon Carcinoma stained with Isocitrate Dehydrogenase 1/IDH1 Mouse Monoclonal Antibody (Isocitrate Dehydrogenase 1/IDH1/1152).
Immunohistochemistry-Paraffin: Isocitrate Dehydrogenase 1/IDH1 Antibody (IDH1/1152) [NBP2-45156]

Immunohistochemistry-Paraffin: Isocitrate Dehydrogenase 1/IDH1 Antibody (IDH1/1152) [NBP2-45156]

Immunohistochemistry-Paraffin: Isocitrate Dehydrogenase 1/IDH1 Antibody (IDH1/1152) [NBP2-45156] - Human Colon Carcinoma stained with IDH1 Monoclonal Antibody (IDH1/1152).
Isocitrate Dehydrogenase 1/IDH1 Antibody (IDH1/1152)

Immunohistochemistry-Paraffin: Isocitrate Dehydrogenase 1/IDH1 Antibody (IDH1/1152) [NBP2-45156] -

Immunohistochemistry-Paraffin: Isocitrate Dehydrogenase 1/IDH1 Antibody (IDH1/1152) [NBP2-45156] - Human Prostate Carcinoma stained with IDH1 Monoclonal Antibody (IDH1/1152).
Isocitrate Dehydrogenase 1/IDH1 Antibody (IDH1/1152) Western Blot: Isocitrate Dehydrogenase 1/IDH1 Antibody (IDH1/1152) [NBP2-45156] -

Western Blot: Isocitrate Dehydrogenase 1/IDH1 Antibody (IDH1/1152) [NBP2-45156] -

Western Blot Analysis of Human Kidney tissue lysate using Isocitrate Dehydrogenase 1/IDH1 Antibody (IDH1/1152).
Isocitrate Dehydrogenase 1/IDH1 Antibody (IDH1/1152) Western Blot: Isocitrate Dehydrogenase 1/IDH1 Antibody (IDH1/1152) [NBP2-45156] -

Western Blot: Isocitrate Dehydrogenase 1/IDH1 Antibody (IDH1/1152) [NBP2-45156] -

Western Blot Analysis of A-549 lysate using Isocitrate Dehydrogenase 1/IDH1 Antibody (IDH1/1152).

Applications for Isocitrate Dehydrogenase 1/IDH1 Antibody (IDH1/1152)

Application
Recommended Usage

Flow Cytometry

1-2 ug/million cells

Immunocytochemistry/ Immunofluorescence

1-2 ug/ml

Immunohistochemistry-Paraffin

1-2 ug/ml

Western Blot

1-2 ug/ml
Application Notes
Immunohistochemistry (Formalin-fixed): 1-2ug/ml for 30 minutes at RT. Staining of formalin-fixed tissues requires heating tissue sections in 10mM Tris with 1mM EDTA, pH 9.0, for 45 min at 95C followed by cooling at RT for 20 minutes.
Optimal dilution for a specific application should be determined.

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Flow Cytometry

Formulation, Preparation, and Storage

Purification

Protein A or G purified

Formulation

1mM PBS with 0.05% BSA

Preservative

0.05% Sodium Azide

Concentration

0.2 mg/ml

Shipping

The product is shipped with polar packs. Upon receipt, store it immediately at the temperature recommended below.

Stability & Storage

Store at 4C.

Background: Isocitrate Dehydrogenase 1/IDH1

Isocitrate dehydrogenases catalyze the oxidative decarboxylation of isocitrate to 2-oxoglutarate. These enzymes belong to two distinct subclasses, one of which utilizes NAD(+) as the electron acceptor and the other NADP(+). Five isocitrate dehydrogenases have been reported: three NAD(+)-dependent isocitrate dehydrogenases, which localize to the mitochondrial matrix, and two NADP(+)-dependent isocitrate dehydrogenases, one of which is mitochondrial and the other predominantly cytosolic. Each NADP(+)-dependent isozyme is a homodimer. The protein encoded by this gene is the NADP(+)-dependent isocitrate dehydrogenase found in the cytoplasm and peroxisomes. It contains the PTS-1 peroxisomal targeting signal sequence. The presence of this enzyme in peroxisomes suggests roles in the regeneration of NADPH for intraperoxisomal reductions, such as the conversion of 2, 4-dienoyl-CoAs to 3-enoyl-CoAs, as well as in peroxisomal reactions that consume 2-oxoglutarate, namely the alpha-hydroxylation of phytanic acid. The cytoplasmic enzyme serves a significant role in cytoplasmic NADPH production. [provided by RefSeq]

Alternate Names

IDCD, IDH1, IDP, IDPC, PICD

Gene Symbol

IDH1

UniProt

O75874

Additional Isocitrate Dehydrogenase 1/IDH1 Products

Product Documents for Isocitrate Dehydrogenase 1/IDH1 Antibody (IDH1/1152)

Certificate of Analysis

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Product Specific Notices for Isocitrate Dehydrogenase 1/IDH1 Antibody (IDH1/1152)

This product is for research use only and is not approved for use in humans or in clinical diagnosis. Primary Antibodies are guaranteed for 1 year from date of receipt.

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Protocols

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