Isocitrate Dehydrogenase 1/IDH1 Antibody - BSA Free

Novus Biologicals | Catalog # NBP1-31599

Novus Biologicals
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Key Product Details

Validated by

Knockout/Knockdown

Species Reactivity

Validated:

Human, Mouse, Rat

Cited:

Mouse

Predicted:

Rhesus Macaque (100%). Backed by our 100% Guarantee.

Applications

Validated:

Knockout Validated, Immunohistochemistry, Immunohistochemistry-Paraffin, Western Blot, Immunoprecipitation, Knockdown Validated

Cited:

Western Blot

Label

Unconjugated

Antibody Source

Polyclonal Rabbit IgG

Format

BSA Free
View all Isocitrate Dehydrogenase 1/IDH1 Primary Antibodies »

Product Specifications

Immunogen

Recombinant protein encompassing a sequence within the center region of human Isocitrate Dehydrogenase 1/IDH1. The exact sequence is proprietary.

Localization

Cytoplasm, Peroxisome

Clonality

Polyclonal

Host

Rabbit

Isotype

IgG

Theoretical MW

47 kDa.
Disclaimer note: The observed molecular weight of the protein may vary from the listed predicted molecular weight due to post translational modifications, post translation cleavages, relative charges, and other experimental factors.

Scientific Data Images for Isocitrate Dehydrogenase 1/IDH1 Antibody - BSA Free

Knockdown Validated: Isocitrate Dehydrogenase 1/IDH1 Antibody [NBP1-31599]

Western Blot: Isocitrate Dehydrogenase 1/IDH1 Antibody [NBP1-31599]

Western Blot: Isocitrate Dehydrogenase 1/IDH1 Antibody [NBP1-31599] - Non-transfected (-) and transfected (+) HepG2 whole cell extracts (30 ug) were separated by 10% SDS-PAGE, and the membrane was blotted with IDH1 antibody. The HRP-conjugated anti-rabbit IgG antibody was used to detect the primary antibody.
Western Blot: Isocitrate Dehydrogenase 1/IDH1 Antibody [NBP1-31599]

Western Blot: Isocitrate Dehydrogenase 1/IDH1 Antibody [NBP1-31599]

Western Blot: Isocitrate Dehydrogenase 1/IDH1 Antibody [NBP1-31599] - Non-transfected (-) and transfected (+) 293T whole cell extracts (30 ug) were separated by 10% SDS-PAGE, and the membrane was blotted with IDH1 antibody diluted at 1:5000. The HRP-conjugated anti-rabbit IgG antibody (NBP2-19301) was used to detect the primary antibody
Western Blot: Isocitrate Dehydrogenase 1/IDH1 Antibody [NBP1-31599]

Western Blot: Isocitrate Dehydrogenase 1/IDH1 Antibody [NBP1-31599]

Western Blot: Isocitrate Dehydrogenase 1/IDH1 Antibody [NBP1-31599] - A. 30 ug Neuro2A whole cell extract B. 30 ug C8D30 whole cell extractC. 30 ug NIH-3T3 whole cell extract D. 30 ug Raw 264.7 whole cell extract E. 30 ug C2Cl2 whole cell extract 10 % SDS-PAGE IDH1 antibody dilution: 1:1000
Immunohistochemistry-Paraffin: Isocitrate Dehydrogenase 1/IDH1 Antibody [NBP1-31599]

Immunohistochemistry-Paraffin: Isocitrate Dehydrogenase 1/IDH1 Antibody [NBP1-31599]

Immunohistochemistry-Paraffin: Isocitrate dehydrogenase Antibody [NBP1-31599] - Paraffin-embedded colon ca, using antibody at 1:100 dilution.
Immunoprecipitation: Isocitrate Dehydrogenase 1/IDH1 Antibody [NBP1-31599]

Immunoprecipitation: Isocitrate Dehydrogenase 1/IDH1 Antibody [NBP1-31599]

Immunoprecipitation: Isocitrate Dehydrogenase 1/IDH1 Antibody [NBP1-31599] - HepG2 whole cell lysate/extract A : 30 ug whole cell lysate/extract of IDH1 protein expressing HepG2 cells B : Control with 2.5 ug of pre-immune rabbit IgG C : Immunoprecipitation of IDH-1 protein by 2.5 ug of IDH-1 antibody 10% SDS-PAGE The immunoprecipitated IDH-1 protein was detected by IDH-1 antibody diluted at 1 : 1000. EasyBlot anti-rabbit IgG (HRP) was used as a secondary reagent.
Isocitrate Dehydrogenase 1/IDH1 Antibody

Immunohistochemistry-Paraffin: Isocitrate Dehydrogenase 1/IDH1 Antibody [NBP1-31599] -

Immunohistochemistry-Paraffin: Isocitrate Dehydrogenase 1/IDH1 Antibody [NBP1-31599] - Isocitrate Dehydrogenase 1/IDH1 antibody detects Isocitrate Dehydrogenase 1/IDH1 protein at cytoplasm by immunohistochemical analysis.
Sample: Paraffin-embedded rat colon.
Isocitrate Dehydrogenase 1/IDH1 stained by Isocitrate Dehydrogenase 1/IDH1 antibody (NBP1-31599) diluted at 1:1000.
Antigen Retrieval: Citrate buffer, pH 6.0, 15 min
Isocitrate Dehydrogenase 1/IDH1 Antibody

Immunohistochemistry-Paraffin: Isocitrate Dehydrogenase 1/IDH1 Antibody [NBP1-31599] -

Immunohistochemistry-Paraffin: Isocitrate Dehydrogenase 1/IDH1 Antibody [NBP1-31599] - Isocitrate Dehydrogenase 1/IDH1 antibody detects Isocitrate Dehydrogenase 1/IDH1 protein at cytoplasm by immunohistochemical analysis.
Sample: Paraffin-embedded rat duodenum.
Isocitrate Dehydrogenase 1/IDH1 stained by Isocitrate Dehydrogenase 1/IDH1 antibody (NBP1-31599) diluted at 1:1000.
Antigen Retrieval: Citrate buffer, pH 6.0, 15 min
Isocitrate Dehydrogenase 1/IDH1 Antibody

Immunohistochemistry-Paraffin: Isocitrate Dehydrogenase 1/IDH1 Antibody [NBP1-31599] -

Immunohistochemistry-Paraffin: Isocitrate Dehydrogenase 1/IDH1 Antibody [NBP1-31599] - Isocitrate Dehydrogenase 1/IDH1 antibody detects Isocitrate Dehydrogenase 1/IDH1 protein at cytoplasm by immunohistochemical analysis.
Sample: Paraffin-embedded mouse intestine.
Isocitrate Dehydrogenase 1/IDH1 stained by Isocitrate Dehydrogenase 1/IDH1 antibody (NBP1-31599) diluted at 1:1000.
Antigen Retrieval: Citrate buffer, pH 6.0, 15 min
Isocitrate Dehydrogenase 1/IDH1 Antibody

Immunohistochemistry-Paraffin: Isocitrate Dehydrogenase 1/IDH1 Antibody [NBP1-31599] -

Immunohistochemistry-Paraffin: Isocitrate Dehydrogenase 1/IDH1 Antibody [NBP1-31599] - Isocitrate Dehydrogenase 1/IDH1 antibody detects Isocitrate Dehydrogenase 1/IDH1 protein at cytoplasm by immunohistochemical analysis.
Sample: Paraffin-embedded mouse liver.
Isocitrate Dehydrogenase 1/IDH1 stained by Isocitrate Dehydrogenase 1/IDH1 antibody (NBP1-31599) diluted at 1:1000.
Antigen Retrieval: Citrate buffer, pH 6.0, 15 min
Isocitrate Dehydrogenase 1/IDH1 Antibody

Immunohistochemistry-Paraffin: Isocitrate Dehydrogenase 1/IDH1 Antibody [NBP1-31599] -

Immunohistochemistry-Paraffin: Isocitrate Dehydrogenase 1/IDH1 Antibody [NBP1-31599] - Isocitrate Dehydrogenase 1/IDH1 antibody detects Isocitrate Dehydrogenase 1/IDH1 protein at cytoplasm by immunohistochemical analysis.
Sample: Paraffin-embedded mouse brain.
Isocitrate Dehydrogenase 1/IDH1 stained by Isocitrate Dehydrogenase 1/IDH1 antibody (NBP1-31599) diluted at 1:1000.
Antigen Retrieval: Citrate buffer, pH 6.0, 15 min
Isocitrate Dehydrogenase 1/IDH1 Antibody - BSA Free

Western Blot: Isocitrate Dehydrogenase 1/IDH1 Antibody [NBP1-31599] -

Wild-type (WT) and Isocitrate Dehydrogenase 1/IDH1 knockout (KO) HeLa cell extracts (30 ug) were separated by 10% SDS-PAGE, and the membrane was blotted with Isocitrate Dehydrogenase 1/IDH1 antibody (NBP1-31599) diluted at 1:500. The HRP-conjugated anti-rabbit IgG antibody was used to detect the primary antibody, and the signal was developed with Trident ECL plus-Enhanced.
Isocitrate Dehydrogenase 1/IDH1 Antibody - BSA Free

Western Blot: Isocitrate Dehydrogenase 1/IDH1 Antibody [NBP1-31599] -

Various whole cell extracts (30 ug) were separated by 10% SDS-PAGE, and the membrane was blotted with Isocitrate Dehydrogenase 1/IDH1 antibody (NBP1-31599) diluted at 1:1000. The HRP-conjugated anti-rabbit IgG antibody was used to detect the primary antibody.
Isocitrate Dehydrogenase 1/IDH1 Antibody - BSA Free

Western Blot: Isocitrate Dehydrogenase 1/IDH1 Antibody [NBP1-31599] -

Various tissue extracts (50 ug) were separated by 10% SDS-PAGE, and the membrane was blotted with Isocitrate Dehydrogenase 1/IDH1 antibody (NBP1-31599) diluted at 1:1000. The HRP-conjugated anti-rabbit IgG antibody was used to detect the primary antibody.

Applications for Isocitrate Dehydrogenase 1/IDH1 Antibody - BSA Free

Application
Recommended Usage

Immunohistochemistry

1:1000-1:10000

Immunohistochemistry-Paraffin

1:100-1:1000

Immunoprecipitation

1:100-1:1000

Western Blot

1:1000-1:10000

Reviewed Applications

Read 1 review rated 5 using NBP1-31599 in the following applications:

Formulation, Preparation, and Storage

Purification

Antigen Affinity-purified

Formulation

PBS, 20% Glycerol

Format

BSA Free

Preservative

0.025% Proclin 300

Concentration

Concentrations vary lot to lot. See vial label for concentration. If unlisted please contact technical services.

Shipping

The product is shipped with polar packs. Upon receipt, store it immediately at the temperature recommended below.

Stability & Storage

Aliquot and store at -20C or -80C. Avoid freeze-thaw cycles.

Background: Isocitrate Dehydrogenase 1/IDH1

Isocitrate dehydrogenases catalyze the oxidative decarboxylation of isocitrate to 2-oxoglutarate. These enzymes belong to two distinct subclasses, one of which utilizes NAD(+) as the electron acceptor and the other NADP(+). Five isocitrate dehydrogenases have been reported: three NAD(+)-dependent isocitrate dehydrogenases, which localize to the mitochondrial matrix, and two NADP(+)-dependent isocitrate dehydrogenases, one of which is mitochondrial and the other predominantly cytosolic. Each NADP(+)-dependent isozyme is a homodimer. The protein encoded by this gene is the NADP(+)-dependent isocitrate dehydrogenase found in the cytoplasm and peroxisomes. It contains the PTS-1 peroxisomal targeting signal sequence. The presence of this enzyme in peroxisomes suggests roles in the regeneration of NADPH for intraperoxisomal reductions, such as the conversion of 2, 4-dienoyl-CoAs to 3-enoyl-CoAs, as well as in peroxisomal reactions that consume 2-oxoglutarate, namely the alpha-hydroxylation of phytanic acid. The cytoplasmic enzyme serves a significant role in cytoplasmic NADPH production.

Alternate Names

IDCD, IDH1, IDP, IDPC, PICD

Gene Symbol

IDH1

Additional Isocitrate Dehydrogenase 1/IDH1 Products

Product Documents for Isocitrate Dehydrogenase 1/IDH1 Antibody - BSA Free

Certificate of Analysis

To download a Certificate of Analysis, please enter a lot or batch number in the search box below.

Product Specific Notices for Isocitrate Dehydrogenase 1/IDH1 Antibody - BSA Free

This product is for research use only and is not approved for use in humans or in clinical diagnosis. Primary Antibodies are guaranteed for 1 year from date of receipt.

Citations for Isocitrate Dehydrogenase 1/IDH1 Antibody - BSA Free

Customer Reviews for Isocitrate Dehydrogenase 1/IDH1 Antibody - BSA Free (1)

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  • Isocitrate Dehydrogenase 1/IDH1 Antibody
    Name: Anonymous
    Application: Western Blot
    Sample Tested: fibroblasts
    Species: Human
    Verified Customer | Posted 12/11/2023
    WB IDH1
    Isocitrate Dehydrogenase 1/IDH1 Antibody - BSA Free NBP1-31599

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