Jagged 1 Antibody (OTI3A10) - Azide and BSA Free

Novus Biologicals | Catalog # NBP2-70115

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Key Product Details

Species Reactivity

Human, Mouse, Rat

Applications

Immunohistochemistry, Immunohistochemistry-Paraffin, Western Blot

Label

Unconjugated

Antibody Source

Monoclonal Mouse IgG2A Clone # OTI3A10

Format

Azide and BSA Free
View all Jagged 1 Primary Antibodies »

Product Specifications

Immunogen

Human recombinant protein fragment corresponding to amino acids 523-759 of human JAG1(NP_000205) produced in E.coli.

Reactivity Notes

Please note that this antibody is reactive to Mouse and derived from the same host, Mouse. Mouse-On-Mouse blocking reagent may be needed for IHC and ICC experiments to reduce high background signal. You can find these reagents under catalog numbers PK-2200-NB and MP-2400-NB. Please contact Technical Support if you have any questions.

Clonality

Monoclonal

Host

Mouse

Isotype

IgG2A

Theoretical MW

133.6 kDa.
Disclaimer note: The observed molecular weight of the protein may vary from the listed predicted molecular weight due to post translational modifications, post translation cleavages, relative charges, and other experimental factors.

Scientific Data Images for Jagged 1 Antibody (OTI3A10) - Azide and BSA Free

Western Blot: Jagged 1 Antibody (OTI3A10)Azide and BSA Free [NBP2-70115]

Western Blot: Jagged 1 Antibody (OTI3A10)Azide and BSA Free [NBP2-70115]

Western Blot: Jagged 1 Antibody (OTI3A10) - Azide and BSA Free [NBP2-70115] - Analysis of HEK293T cells were transfected with the pCMV6-ENTRY control (Left lane) or pCMV6-ENTRY Jagged 1.
Immunohistochemistry: Jagged 1 Antibody (OTI3A10) - Azide and BSA Free [NBP2-70115]

Immunohistochemistry: Jagged 1 Antibody (OTI3A10) - Azide and BSA Free [NBP2-70115]

Immunohistochemistry: Jagged 1 Antibody (OTI3A10) - Azide and BSA Free [NBP2-70115] - Analysis of Human prostate tissue. (Heat-induced epitope retrieval by 1mM EDTA in 10mM Tris buffer (pH8.5) at 120C for 3 min)
Immunohistochemistry: Jagged 1 Antibody (OTI3A10) - Azide and BSA Free [NBP2-70115]

Immunohistochemistry: Jagged 1 Antibody (OTI3A10) - Azide and BSA Free [NBP2-70115]

Immunohistochemistry: Jagged 1 Antibody (OTI3A10) - Azide and BSA Free [NBP2-70115] - Analysis of Adenocarcinoma of Human ovary tissue. (Heat-induced epitope retrieval by 1mM EDTA in 10mM Tris buffer (pH8.5) at 120C for 3 min)
Immunohistochemistry: Jagged 1 Antibody (OTI3A10) - Azide and BSA Free [NBP2-70115]

Immunohistochemistry: Jagged 1 Antibody (OTI3A10) - Azide and BSA Free [NBP2-70115]

Immunohistochemistry: Jagged 1 Antibody (OTI3A10) - Azide and BSA Free [NBP2-70115] - Analysis of Carcinoma of Human liver tissue.(Heat-induced epitope retrieval by 1mM EDTA in 10mM Tris buffer (pH8.5) at 120C for 3 min)
Immunohistochemistry: Jagged 1 Antibody (OTI3A10) - Azide and BSA Free [NBP2-70115]

Immunohistochemistry: Jagged 1 Antibody (OTI3A10) - Azide and BSA Free [NBP2-70115]

Immunohistochemistry: Jagged 1 Antibody (OTI3A10) - Azide and BSA Free [NBP2-70115] - Analysis of Carcinoma of Human prostate tissue. (Heat-induced epitope retrieval by 1mM EDTA in 10mM Tris buffer (pH8.5) at 120C for 3 min)
Immunohistochemistry: Jagged 1 Antibody (OTI3A10) - Azide and BSA Free [NBP2-70115]

Immunohistochemistry: Jagged 1 Antibody (OTI3A10) - Azide and BSA Free [NBP2-70115]

Immunohistochemistry: Jagged 1 Antibody (OTI3A10) - Azide and BSA Free [NBP2-70115] - Analysis of Human lymph node tissue. (Heat-induced epitope retrieval by 1mM EDTA in 10mM Tris buffer (pH8.5) at 120C for 3 min)
Immunohistochemistry: Jagged 1 Antibody (OTI3A10) - Azide and BSA Free [NBP2-70115]

Immunohistochemistry: Jagged 1 Antibody (OTI3A10) - Azide and BSA Free [NBP2-70115]

Immunohistochemistry: Jagged 1 Antibody (OTI3A10) - Azide and BSA Free [NBP2-70115] - Analysis of Human pancreas tissue. (Heat-induced epitope retrieval by 1mM EDTA in 10mM Tris buffer (pH8.5) at 120C for 3 min)
Immunohistochemistry: Jagged 1 Antibody (OTI3A10) - Azide and BSA Free [NBP2-70115]

Immunohistochemistry: Jagged 1 Antibody (OTI3A10) - Azide and BSA Free [NBP2-70115]

Immunohistochemistry: Jagged 1 Antibody (OTI3A10) - Azide and BSA Free [NBP2-70115] - Analysis of Human tonsil tissue. (Heat-induced epitope retrieval by 1mM EDTA in 10mM Tris buffer (pH8.5) at 120C for 3 min)

Applications for Jagged 1 Antibody (OTI3A10) - Azide and BSA Free

Application
Recommended Usage

Immunohistochemistry

1:150

Western Blot

1:2000

Formulation, Preparation, and Storage

Purification

Immunogen affinity purified

Reconstitution

we recommend adding 100uL distilled water to a final antibody concentration of about 1 mg/mL. To use this carrier-free antibody for conjugation experiment, we strongly recommend performing another round of desalting process.

Formulation

Lyophilized from PBS (pH 7.3) with 8% Trehalose

Format

Azide and BSA Free

Preservative

No Preservative

Concentration

LYOPH mg/ml

Shipping

The product is shipped with polar packs. Upon receipt, store it immediately at the temperature recommended below.

Stability & Storage

Store at -20C. Avoid freeze-thaw cycles.

Calculators

The reconstitution calculator allows you to quickly calculate the volume of a reagent to reconstitute your vial. Simply enter the mass of reagent and the target concentration and the calculator will determine the rest.

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Background: Jagged 1

The jagged 1 protein encoded by JAG1 is the human homolog of the Drosophilia jagged protein. Human jagged 1 is theligand for the receptor notch 1, the latter a human homolog of the Drosophilia jagged receptor notch. Mutations thatalter the jagged 1 protein cause Alagille syndrome. Jagged 1 signalling through notch 1 has also been shown to play arole in hematopoiesis. (provided by RefSeq)

Alternate Names

CD339, JAG1

Gene Symbol

JAG1

Additional Jagged 1 Products

Product Documents for Jagged 1 Antibody (OTI3A10) - Azide and BSA Free

Certificate of Analysis

To download a Certificate of Analysis, please enter a lot or batch number in the search box below.

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Product Specific Notices for Jagged 1 Antibody (OTI3A10) - Azide and BSA Free

This product is for research use only and is not approved for use in humans or in clinical diagnosis. Primary Antibodies are guaranteed for 1 year from date of receipt.

Related Research Areas

Cancer Biomarkers
Notch Pathway

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Protocols

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FAQs

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