JAM-B/VE-JAM Antibody (1G4) - Azide and BSA Free

Name: JAM-B/VE-JAM Antibody (1G4) - Azide and BSA Free
Brand: Novus Biologicals
SKU: H00058494-M01
Price: 479 USD
Availability: InStock

Novus Biologicals | Catalog # H00058494-M01

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Product Specifications
Scientific Data
Applications
Preparation & Storage
Background
Product Documents
Specific Notices
Research Areas

Key Product Details

Species Reactivity

Human

Applications

Immunohistochemistry, Immunohistochemistry-Paraffin, Western Blot, ELISA, Sandwich ELISA

Label

Unconjugated

Antibody Source

Monoclonal Mouse IgG₁ kappa Clone # 1G4

Format

Azide and BSA Free

View all JAM-B/VE-JAM Primary Antibodies »

Product Specifications

Immunogen

JAM2 (AAH17779, 29 a.a. ~ 298 a.a) full-length recombinant protein with GST tag. MW of the GST tag alone is 26 KDa. FSAPKDQQVVTAVEYQEAILACKTPKKTVSSRLEWKKLGRSVSFVYYQQTLQGDFKNRAEMIDFNIRIKNVTRSDAGKYRCEVSAPSEQGQNLEEDTVTLEVLVAPAVPSCEVPSSALSGTVVELRCQDKEGNPAPEYTWFKDGIRLLENPRLGSQSTNSSYTMNTKTGTLQFNTVSKLDTGEYSCEARNSVGYRRCPGKRMQVDDLNISGIIAAVVVVALVISVCGLGVCYAQRKGYFSKETSFQKSNSSSKATTMSENDFKHTKSFII

Reactivity Notes

Human. Other species not tested.

Specificity

JAM2 - junctional adhesion molecule 2

Clonality

Monoclonal

Host

Mouse

Isotype

IgG₁ kappa

Scientific Data Images for JAM-B/VE-JAM Antibody (1G4) - Azide and BSA Free

Western Blot: JAM-B/VE-JAM Antibody (1G4) [H00058494-M01]

Western Blot: JAM-B/VE-JAM Antibody (1G4) [H00058494-M01] - JAM2 monoclonal antibody (M01), clone 1G4. Analysis of JAM2 expression in human ovarian cancer.

Immunohistochemistry-Paraffin: JAM-B/VE-JAM Antibody (1G4) [H00058494-M01]

Immunohistochemistry-Paraffin: JAM-B/VE-JAM Antibody (1G4) [H00058494-M01] - Analysis of monoclonal antibody to JAM2 on formalin-fixed paraffin-embedded human malignant lymphoma, diffuse large B tissue. Antibody concentration 1 ug/ml.

Western Blot: JAM-B/VE-JAM Antibody (1G4) [H00058494-M01]

Western Blot: JAM-B/VE-JAM Antibody (1G4) [H00058494-M01] - JAM2 expression in LNCaP ( Cat # L004V1 ).

Western Blot: JAM-B/VE-JAM Antibody (1G4) [H00058494-M01]

Western Blot: JAM-B/VE-JAM Antibody (1G4) [H00058494-M01] - JAM2 monoclonal antibody (M01), clone 1G4. Analysis of JAM2 expression in HL-60.

Western Blot: JAM-B/VE-JAM Antibody (1G4) [H00058494-M01]

Western Blot: JAM-B/VE-JAM Antibody (1G4) [H00058494-M01] - Analysis of JAM2 expression in transfected 293T cell line by JAM2 monoclonal antibody (M01), clone 1G4.Lane 1: JAM2 transfected lysate (Predicted MW: 33.2 KDa).Lane 2: Non-transfected lysate.

ELISA: JAM-B/VE-JAM Antibody (1G4) [H00058494-M01]

ELISA: JAM-B/VE-JAM Antibody (1G4) [H00058494-M01] - Detection limit for recombinant GST tagged JAM2 is approximately 3ng/ml as a capture antibody.

Sandwich ELISA: JAM-B/VE-JAM Antibody (1G4) [H00058494-M01] - Detection limit for recombinant GST tagged JAM2 is approximately 0.1ng/ml as a capture antibody.

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Applications for JAM-B/VE-JAM Antibody (1G4) - Azide and BSA Free

Application

Recommended Usage

Western Blot

1:500

Application Notes

Antibody reactive against cell lysate and recombinant protein for Western Blot. Has also been used for immunohistochemistry (paraffin) and ELISA.

Formulation, Preparation, and Storage

Purification

IgG purified

Formulation

In 1x PBS, pH 7.4

Format

Azide and BSA Free

Preservative

No Preservative

Concentration

Concentrations vary lot to lot. See vial label for concentration. If unlisted please contact technical services.

Shipping

The product is shipped with polar packs. Upon receipt, store it immediately at the temperature recommended below.

Stability & Storage

Aliquot and store at -20C or -80C. Avoid freeze-thaw cycles.

Background: JAM-B/VE-JAM

Tight junctions represent one mode of cell-to-cell adhesion in epithelial or endothelial cell sheets, forming continuous seals around cells and serving as a physical barrier to prevent solutes and water from passing freely through the paracellular space. The protein encoded by this immunoglobulin superfamily gene member is localized in the tight junctions between high endothelial cells. It acts as an adhesive ligand for interacting with a variety of immune cell types and may play a role in lymphocyte homing to secondary lymphoid organs.

Long Name

Junctional Adhesion Molecule B

Alternate Names

CD322, JAM2, JAMB, VE-JAM

Entrez Gene IDs

58494 (Human)

Gene Symbol

JAM2

OMIM

606870 (Human)

UniProt

AAH17779 (Human)

Additional JAM-B/VE-JAM Products

Product Documents for JAM-B/VE-JAM Antibody (1G4) - Azide and BSA Free

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Certificate of Analysis

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Product Specific Notices for JAM-B/VE-JAM Antibody (1G4) - Azide and BSA Free

This product is produced by and distributed for Abnova, a company based in Taiwan.

This product is for research use only and is not approved for use in humans or in clinical diagnosis. Primary Antibodies are guaranteed for 1 year from date of receipt.