JMJD6/PSR Antibody

Novus Biologicals | Catalog # NBP1-71693

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Key Product Details

Species Reactivity

Validated:

Human

Predicted:

Bovine (98%), Mouse (98%), Rat (98%), Xenopus (91%), Zebrafish (91%). Backed by our 100% Guarantee.

Applications

Western Blot, Immunocytochemistry/ Immunofluorescence

Label

Unconjugated

Antibody Source

Polyclonal Rabbit IgG
View all JMJD6/PSR Primary Antibodies »

Product Specifications

Immunogen

A genomic peptide made to an internal region of the human JMJD6 protein (within residues 150-350). [Swiss-Prot Q6NYC1]

Reactivity Notes

Immunogen has 98% identity with mouse, rat and bovine. Immunogen has 91% identity to Xenopus and Zebrafish.

Localization

Nucleus.

Clonality

Polyclonal

Host

Rabbit

Isotype

IgG

Scientific Data Images for JMJD6/PSR Antibody

Western Blot: JMJD6/PSR Antibody [NBP1-71693]

Western Blot: JMJD6/PSR Antibody [NBP1-71693]

Western Blot: JMJD6/PSR Antibody [NBP1-71693] - Analysis of JMJD6 in A431 cell lysate.
Immunocytochemistry/ Immunofluorescence: JMJD6/PSR Antibody [NBP1-71693]

Immunocytochemistry/ Immunofluorescence: JMJD6/PSR Antibody [NBP1-71693]

Immunocytochemistry/Immunofluorescence: JMJD6/PSR Antibody [NBP1-71693] - JMJD6 antibody was tested at 10 ug/ml in A431 cells with FITC (green). Nuclei (Blue) were counterstained with Dapi (blue).

Applications for JMJD6/PSR Antibody

Application
Recommended Usage

Immunocytochemistry/ Immunofluorescence

1:50-1:200

Western Blot

1:2000
Application Notes
This JMJD6 antibody is useful for ICC and Western Blot, where a band is seen ~45 kDa.

Reviewed Applications

Read 1 review rated 5 using NBP1-71693 in the following applications:

Formulation, Preparation, and Storage

Purification

Immunogen affinity purified

Formulation

PBS, 0.1% BSA, and 50% Glycerol

Preservative

0.05% Sodium Azide

Concentration

0.2 mg/ml

Shipping

The product is shipped with polar packs. Upon receipt, store it immediately at the temperature recommended below.

Stability & Storage

Store at 4C short term. Aliquot and store at -20C long term. Avoid freeze-thaw cycles.

Background: JMJD6/PSR

Jumonji domain containing 6 is a histone demethylating enzyme, which is active on arginine residues, but not lysines. Its activity destabilizes heterochromatin structures and activates gene expressions in euchromatin. In endothelial cells, the activity of JMJD6 is necessary for angiogenesis. If JMJD6 expression is reduced, the VEGF-receptor (Flt-1) is incorrectly spliced, leading to increased soluble Flt-1 which binds VEGF and PlGF and inhibits angiogenesis. JMJD6 also hydroxylates RNA splicing factors, and is important in embryonic differentiation. It most likely interacts specifically with single-stranded RNA, which may contribute to its early differentiation importance.

Long Name

Jumonji Domain Containing 6/Phosphatidylserine Receptor

Alternate Names

PSR, PTDSR

Entrez Gene IDs

23210 (Human)

Gene Symbol

JMJD6

UniProt

Q6NYC1 (Human)

Additional JMJD6/PSR Products

Product Documents for JMJD6/PSR Antibody

Certificate of Analysis

To download a Certificate of Analysis, please enter a lot or batch number in the search box below.

Download SDS

Product Specific Notices for JMJD6/PSR Antibody

Manufactured by Genomic Antibody Technology™. GAT FAQs

This product is for research use only and is not approved for use in humans or in clinical diagnosis. Primary Antibodies are guaranteed for 1 year from date of receipt.

Related Research Areas

Apoptosis
Redox Enzymes

Customer Reviews for JMJD6/PSR Antibody (1)

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  • Name: Anonymous
    Application: Western Blot
    Sample Tested: MDA-MB-435 cell lysates
    Species: Human
    Verified Customer | Posted 01/11/2012
    JMJD6/PSR Antibody NBP1-71693

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Protocols

View specific protocols for JMJD6/PSR Antibody (NBP1-71693):

JMJD6/PSR Antibody:
Immunocytochemistry Protocol

Culture cells to appropriate density in 35 mm culture dishes or 6-well plates.

1. Remove culture medium and add 10% formalin to the dish. Fix at room temperature for 30 minutes.
2. Remove the formalin and add ice cold methanol. Incubate for 5-10 minutes.
3. Remove methanol and add washing solution (i.e. PBS). Be sure to not let the specimen dry out. Wash three times for 10 minutes.
4. To block nonspecific antibody binding incubate in 10% normal goat serum from 1 hour to overnight at room temperature.
5. Add primary antibody at appropriate dilution and incubate at room temperature from 2 hours to overnight at room temperature.
6. Remove primary antibody and replace with washing solution. Wash three times for 10 minutes.
7. Add secondary antibody at appropriate dilution. Incubate for 1 hour at room temperature.
8. Remove antibody and replace with wash solution, then wash for 10 minutes. Add Hoechst 33258 to wash solution at 1:25,0000 and incubate for 10 minutes. Wash a third time for 10 minutes.
9. Cells can be viewed directly after washing. The plates can also be stored in PBS containing Azide covered in Parafilm (TM). Cells can also be cover-slipped using Fluoromount, with appropriate sealing.

*The above information is only intended as a guide. The researcher should determine what protocol best meets their needs. Please follow safe laboratory procedures.

JMJD6/PSR Antibody:
Western Blot Protocol

1. Perform SDS-PAGE on samples to be analyzed, loading 40 ug of total protein per lane.
2. Transfer proteins to membrane according to the instructions provided by the manufacturer of the membrane and transfer apparatus.
3. Stain according to standard Ponceau S procedure (or similar product) to assess transfer success, and mark molecular weight standards where appropriate.
4. Rinse the blot.
5. Block the membrane using standard blocking buffer for at least 1 hour.
6. Wash the membrane in wash buffer three times for 10 minutes each.
7. Dilute primary antibody in blocking buffer and incubate 1 hour at room temperature.
8. Wash the membrane in wash buffer three times for 10 minutes each.
9. Apply the diluted HRP conjugated secondary antibody in blocking buffer (as per manufacturers instructions) and incubate 1 hour at room temperature.
10. Wash the blot in wash buffer three times for 10 minutes each (this step can be repeated as required to reduce background).
11. Apply the detection reagent of choice in accordance with the manufacturers instructions.

*Note: Tween-20 can be added to the blocking or antibody dilution buffer at a final concentration of 0.05-0.2%.

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FAQs

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