JNJ 47965567

Tocris Bioscience | Catalog # 5299

Potent and selective P2X7 antagonist; brain penetrant
Tocris Bioscience

Key Product Details

Description

Potent and selective P2X7 antagonist; brain penetrant
View all P2X Receptor Antagonists »

Product Description

JNJ 47965567 is a potent and selective P2X7 antagonist (pIC50 values are 8.3, 7.5 and 7.2 for human, mouse and rat receptors respectively). Selective over a panel of 50 other receptors, ion channels and transporters. Reduces BzATP-induced IL-1β release from monocytes in vitro and from rat brain in vivo. Attenuates amphetamine-induced hyperactivity in rats. Brain penetrant.

Product Specifications for JNJ 47965567

Molecular Weight

488.64

Formula

C28H32N4O2S

Storage

Store at +4°C

Purity

≥98% (HPLC)

Chemical Name

2-(Phenylthio)-N-[[tetrahydro-4-(4-phenyl-1-piperazinyl)-2H-pyran-4-yl]methyl-3-pyridinecarboxamide

CAS Number

1428327-31-4

PubChem ID

66553218

InChI Key

XREFXUCWSYMIOG-UHFFFAOYSA-N

SMILES

O=C(NCC4(CCOCC4)N3CCN(C5=CC=CC=C5)CC3)C1=CC=CN=C1SC2=CC=CC=C2

The technical data provided above is for guidance only. For batch specific data refer to the Certificate of Analysis.

Solubility

Solvent Max Conc. mg/mL Max Conc. mM
Solubility
1eq. HCl 24.43 50
DMSO 48.86 100

Preparing Stock Solutions for JNJ 47965567

The following data is based on the product molecular weight 488.64.

Batch specific molecular weights may vary from batch to batch due to the degree of hydration, which all affect the solvent volumes required to prepare stock solutions.

Select a batch to recalculate based on the batch molecular weight:

Concentration / Solvent Volume / Mass 1 mg 5 mg 10 mg
1 mM 2.05 mL 10.23 mL 20.46 mL
5 mM 0.41 mL 2.05 mL 4.09 mL
10 mM 0.20 mL 1.02 mL 2.05 mL
50 mM 0.04 mL 0.20 mL 0.41 mL

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Background References

References are publications that support the biological activity of the product. See our Citations tab to view 33 publications citing the usage of this product.

Product Documents for JNJ 47965567

Certificate of Analysis

To download a Certificate of Analysis, please enter a lot or batch number in the search box below.

Product Specific Notices for JNJ 47965567

For research use only

Customer Reviews for JNJ 47965567 (3)

4.3 out of 5
3 Customer Ratings
5 Stars
67%
4 Stars
0%
3 Stars
33%
2 Stars
0%
1 Stars
0%

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Showing  1 - 3 of 3 reviews Showing All
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  • Good inhibitor
    Name: Stefan Uderhardt
    Species: Mouse
    Assay Type: In Vivo
    Cell Line/Tissue: phagocyte
    Verified Customer | Posted 06/06/2020
    DMSO dilution 100 μM
    JNJ 47965567 5299
  • In vivo usage
    Name: Spandita Dutta
    Species: Mouse
    Assay Type: In Vivo
    Cell Line/Tissue: Hippocampal neurons
    Verified Customer | Posted 08/07/2018
    Followed publication by Bhattacharya et al, Br J Pharmacol. 2013 Oct;170(3):624-40. doi: 10.1111/bph.12314 for in vivo and in vitro usage.
    I have used this product emulsified in 30% sulphoxymethylester, and injected them sub-cutaneously to study IL-1beta release mechanism in the hippocampus of the brain. I am currently working on its effect on hippocampal neurons in culture.
    JNJ 47965567 5299
  • P2X7 blocker
    Name: Anonymous
    Species: Mouse
    Assay Type: Ex Vivo
    Verified Customer | Posted 01/31/2018
    Used to block a potentially P2X7-dependent process, but no effect was observed. No positive control so unsure if the drug actually blocked its target. More experiments needed.

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