Kallikrein 3/PSA Antibody (5A11E9) - BSA Free
Novus Biologicals | Catalog # NBP1-51541
![Western Blot: Kallikrein 3/PSA Antibody (5A11E9)BSA Free [NBP1-51541]](https://resources.rndsystems.com/images/products/Kallikrein-3-PSA-Antibody-5A11E9-Western-Blot-NBP1-51541-img0012.jpg "Western Blot: Kallikrein 3/PSA Antibody (5A11E9)BSA Free [NBP1-51541]")
Key Product Details
Species Reactivity
Human
Applications
Immunohistochemistry, Immunohistochemistry-Paraffin, Western Blot, ELISA, Flow Cytometry
Label
Unconjugated
Antibody Source
Monoclonal Mouse IgG1 Clone # 5A11E9
Format
BSA Free
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Product Specifications
Immunogen
Purified recombinant fragment of Prostate Specific Antigen (amino acids 26-251) expressed in E. coli. [UniProt# P07288]
Localization
Secreted.
Clonality
Monoclonal
Host
Mouse
Isotype
IgG1
Scientific Data Images for Kallikrein 3/PSA Antibody (5A11E9) - BSA Free
Western Blot: Kallikrein 3/PSA Antibody (5A11E9)BSA Free [NBP1-51541]
Western Blot: Kallikrein 3/PSA Antibody (5A11E9) [NBP1-51541] - Analysis against truncated Prostate Specific Antigen recombinant protein.![Immunohistochemistry-Paraffin: Kallikrein 3/PSA Antibody (5A11E9) - BSA Free [NBP1-51541]](https://resources.rndsystems.com/images/products/Kallikrein-3-PSA-Antibody-5A11E9-Immunohistochemistry-Paraffin-NBP1-51541-img0011.jpg "Immunohistochemistry-Paraffin: Kallikrein 3/PSA Antibody (5A11E9) - BSA Free [NBP1-51541]")
Immunohistochemistry-Paraffin: Kallikrein 3/PSA Antibody (5A11E9) - BSA Free [NBP1-51541]
Immunohistochemistry-Paraffin: Kallikrein 3/PSA Antibody (5A11E9) [NBP1-51541] - Analysis of paraffin-embedded human prostate carcinoma tissues, showing cytoplasmic localization using Prostate Specific Antigen mouse mAb with DAB staining.![Flow Cytometry: Kallikrein 3/PSA Antibody (5A11E9) - BSA Free [NBP1-51541]](https://resources.rndsystems.com/images/products/Kallikrein-3-PSA-Antibody-5A11E9-Flow-Cytometry-NBP1-51541-img0009.jpg "Flow Cytometry: Kallikrein 3/PSA Antibody (5A11E9) - BSA Free [NBP1-51541]")
Flow Cytometry: Kallikrein 3/PSA Antibody (5A11E9) - BSA Free [NBP1-51541]
Flow Cytometry: Kallikrein 3/PSA Antibody (5A11E9) [NBP1-51541] - Flow cytometric analysis of PC-3 cells using Prostate Specific Antigen mouse mAb (right) and negative control (left).![Immunohistochemistry-Paraffin: Kallikrein 3/PSA Antibody (5A11E9) - BSA Free [NBP1-51541]](https://resources.rndsystems.com/images/products/Kallikrein-3-PSA-Antibody-5A11E9-Immunohistochemistry-Paraffin-NBP1-51541-img0010.jpg "Immunohistochemistry-Paraffin: Kallikrein 3/PSA Antibody (5A11E9) - BSA Free [NBP1-51541]")
Immunohistochemistry-Paraffin: Kallikrein 3/PSA Antibody (5A11E9) - BSA Free [NBP1-51541]
Immunohistochemistry-Paraffin: Kallikrein 3/PSA Antibody (5A11E9) [NBP1-51541] - Analysis of paraffin-embedded human prostate tissues using Prostate Specific Antigen mouse mAb with DAB staining.Applications for Kallikrein 3/PSA Antibody (5A11E9) - BSA Free
Application
Recommended Usage
ELISA
1:10000
Flow Cytometry
1:200-1:400
Immunohistochemistry
1:10-1:500
Immunohistochemistry-Paraffin
1:200-1:1000
Western Blot
1:500-1:2000
Application Notes
This Prostate Specific Antigen (5A11E9) antibody is useful for Western blot, Flow Cytometry, Immunohistochemistry on paraffin-embedded sections and ELISA.
Flow Cytometry Panel Builder
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Advanced Features
- Spectra Viewer - Custom analysis of spectra from multiple fluorochromes
- Spillover Popups - Visualize the spectra of individual fluorochromes
- Antigen Density Selector - Match fluorochrome brightness with antigen density
Formulation, Preparation, and Storage
Purification
Protein G purified
Formulation
PBS
Format
BSA Free
Preservative
0.02% Sodium Azide
Concentration
1.0 mg/ml
Shipping
The product is shipped with polar packs. Upon receipt, store it immediately at the temperature recommended below.
Stability & Storage
Store at 4C short term. Aliquot and store at -20C long term. Avoid freeze-thaw cycles.
Background: Kallikrein 3/PSA
Additional Kallikrein 3/PSA Products
Product Documents for Kallikrein 3/PSA Antibody (5A11E9) - BSA Free
Certificate of Analysis
To download a Certificate of Analysis, please enter a lot or batch number in the search box below.
Product Specific Notices for Kallikrein 3/PSA Antibody (5A11E9) - BSA Free
This product is for research use only and is not approved for use in humans or in clinical diagnosis. Primary Antibodies are guaranteed for 1 year from date of receipt.
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Protocols
Find general support by application which include: protocols, troubleshooting, illustrated assays, videos and webinars.
- 7-Amino Actinomycin D (7-AAD) Cell Viability Flow Cytometry Protocol
- Antigen Retrieval Protocol (PIER)
- Antigen Retrieval for Frozen Sections Protocol
- Appropriate Fixation of IHC/ICC Samples
- Cellular Response to Hypoxia Protocols
- Chromogenic IHC Staining of Formalin-Fixed Paraffin-Embedded (FFPE) Tissue Protocol
- Chromogenic Immunohistochemistry Staining of Frozen Tissue
- ClariTSA™ Fluorophore Kits
- Detection & Visualization of Antibody Binding
- ELISA Sample Preparation & Collection Guide
- ELISA Troubleshooting Guide
- Extracellular Membrane Flow Cytometry Protocol
- Flow Cytometry Protocol for Cell Surface Markers
- Flow Cytometry Protocol for Staining Membrane Associated Proteins
- Flow Cytometry Staining Protocols
- Flow Cytometry Troubleshooting Guide
- Fluorescent IHC Staining of Frozen Tissue Protocol
- Graphic Protocol for Heat-induced Epitope Retrieval
- Graphic Protocol for the Preparation and Fluorescent IHC Staining of Frozen Tissue Sections
- Graphic Protocol for the Preparation and Fluorescent IHC Staining of Paraffin-embedded Tissue Sections
- Graphic Protocol for the Preparation of Gelatin-coated Slides for Histological Tissue Sections
- How to Run an R&D Systems DuoSet ELISA
- How to Run an R&D Systems Quantikine ELISA
- How to Run an R&D Systems Quantikine™ QuicKit™ ELISA
- IHC Sample Preparation (Frozen sections vs Paraffin)
- Immunofluorescent IHC Staining of Formalin-Fixed Paraffin-Embedded (FFPE) Tissue Protocol
- Immunohistochemistry (IHC) and Immunocytochemistry (ICC) Protocols
- Immunohistochemistry Frozen Troubleshooting
- Immunohistochemistry Paraffin Troubleshooting
- Intracellular Flow Cytometry Protocol Using Alcohol (Methanol)
- Intracellular Flow Cytometry Protocol Using Detergents
- Intracellular Nuclear Staining Flow Cytometry Protocol Using Detergents
- Intracellular Staining Flow Cytometry Protocol Using Alcohol Permeabilization
- Intracellular Staining Flow Cytometry Protocol Using Detergents to Permeabilize Cells
- Preparing Samples for IHC/ICC Experiments
- Preventing Non-Specific Staining (Non-Specific Binding)
- Primary Antibody Selection & Optimization
- Propidium Iodide Cell Viability Flow Cytometry Protocol
- Protocol for Heat-Induced Epitope Retrieval (HIER)
- Protocol for Liperfluo
- Protocol for Making a 4% Formaldehyde Solution in PBS
- Protocol for VisUCyte™ HRP Polymer Detection Reagent
- Protocol for the Characterization of Human Th22 Cells
- Protocol for the Characterization of Human Th9 Cells
- Protocol for the Preparation & Fixation of Cells on Coverslips
- Protocol for the Preparation and Chromogenic IHC Staining of Frozen Tissue Sections
- Protocol for the Preparation and Chromogenic IHC Staining of Frozen Tissue Sections - Graphic
- Protocol for the Preparation and Chromogenic IHC Staining of Paraffin-embedded Tissue Sections
- Protocol for the Preparation and Chromogenic IHC Staining of Paraffin-embedded Tissue Sections - Graphic
- Protocol for the Preparation and Fluorescent IHC Staining of Frozen Tissue Sections
- Protocol for the Preparation and Fluorescent IHC Staining of Paraffin-embedded Tissue Sections
- Protocol for the Preparation of Gelatin-coated Slides for Histological Tissue Sections
- Protocol: Annexin V and PI Staining by Flow Cytometry
- Protocol: Annexin V and PI Staining for Apoptosis by Flow Cytometry
- Quantikine HS ELISA Kit Assay Principle, Alkaline Phosphatase
- Quantikine HS ELISA Kit Principle, Streptavidin-HRP Polymer
- R&D Systems Quality Control Western Blot Protocol
- Sandwich ELISA (Colorimetric) – Biotin/Streptavidin Detection Protocol
- Sandwich ELISA (Colorimetric) – Direct Detection Protocol
- TUNEL and Active Caspase-3 Detection by IHC/ICC Protocol
- The Importance of IHC/ICC Controls
- Troubleshooting Guide: ELISA
- Troubleshooting Guide: Fluorokine Flow Cytometry Kits
- Troubleshooting Guide: Immunohistochemistry
- Troubleshooting Guide: Western Blot Figures
- Western Blot Conditions
- Western Blot Protocol
- Western Blot Protocol for Cell Lysates
- Western Blot Troubleshooting
- Western Blot Troubleshooting Guide
- View all Protocols, Troubleshooting, Illustrated assays and Webinars
FAQs for Kallikrein 3/PSA Antibody (5A11E9) - BSA Free
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Q: I am looking for a pair of antibodies against TOTAL PSA to be used in a sandwich immunoassay. I saw that you carry a biotiny-Ab (NBP1-44951). I was wondering if it can be used to detect TOTAL PSA and if you recommend a matched pair?
A: A list of our PSA antibodies suitable for use in ELISA can be found using this link. None have been paired specifically with NBP1-44951 but any monoclonal on this list should suffice. I would recommend NBP1-51541 as it is our best selling antibody to this target and should be compatible with NBP1-44951.
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