Kallikrein 3/PSA Antibody (RM323)
Novus Biologicals | Catalog # NBP2-77416
Recombinant Monoclonal Antibody
![Western Blot: Kallikrein 3/PSA Antibody (RM323) [NBP2-77416]](https://resources.rndsystems.com/images/products/Kallikrein-3-PSA-Antibody-RM323-Western-Blot-NBP2-77416-img0002.jpg "Western Blot: Kallikrein 3/PSA Antibody (RM323) [NBP2-77416]")
Loading...
Key Product Details
Species Reactivity
Human
Applications
Immunohistochemistry, Western Blot, ELISA
Label
Unconjugated
Antibody Source
Recombinant Monoclonal Rabbit IgG Clone # RM323 expressed in HEK293
Loading...
Product Specifications
Immunogen
Native Kallikrein 3/PSA protein purified from human seminal fluid
Specificity
This antibody reacts to native human Kallikrein 3/PSA, including both free (unbound) form and in a complex with ACT (alpha 1-antichymotrypsin). It may react to reduced Kallikrein 3/PSA at a higher concentration.
Clonality
Monoclonal
Host
Rabbit
Isotype
IgG
Scientific Data Images for Kallikrein 3/PSA Antibody (RM323)
Western Blot: Kallikrein 3/PSA Antibody (RM323) [NBP2-77416]
Western Blot: Kallikrein 3/PSA Antibody (RM323) [NBP2-77416] - Western Blot of PSA (purified from human seminal fluid), ACT (alpha 1-antichymotrypsin, from human plasma), and PSA-ACT complex, under non-reduced (-) or reduced (+) conditions, using NBP2-77416.![Immunohistochemistry: Kallikrein 3/PSA Antibody (RM323) [NBP2-77416]](https://resources.rndsystems.com/images/products/Kallikrein-3-PSA-Antibody-RM323-Immunohistochemistry-NBP2-77416-img0001.jpg "Immunohistochemistry: Kallikrein 3/PSA Antibody (RM323) [NBP2-77416]")
Immunohistochemistry: Kallikrein 3/PSA Antibody (RM323) [NBP2-77416]
Immunohistochemistry: Kallikrein 3/PSA Antibody (RM323) [NBP2-77416] - Immunohistochemical staining of formalin fixed and paraffin embedded human prostate cancer tissue section using NBP2-77416.
ELISA: Kallikrein 3/PSA Antibody (RM323) [NBP2-77416] -
ELISA: Kallikrein 3/PSA Antibody (RM323) [NBP2-77416] - A titer ELISA of PSA (purified from human seminal fluid). The plate was coated with different amounts of PSA. A serial dilution of NBP2-77416 was used as the primary antibody. An alkaline phosphatase conjugated anti-rabbit IgG as the secondary antibody.Applications for Kallikrein 3/PSA Antibody (RM323)
Application
Recommended Usage
ELISA
1:100 - 1:5000
Immunohistochemistry
1:500 - 1:1000
Western Blot
1:1000 - 1:2500 (non-reducing conditions)
Formulation, Preparation, and Storage
Purification
Protein A purified
Formulation
50% Glycerol/PBS, 1% BSA
Preservative
0.09% Sodium Azide
Concentration
Please see the vial label for concentration. If unlisted please contact technical services.
Shipping
The product is shipped with polar packs. Upon receipt, store it immediately at the temperature recommended below.
Stability & Storage
Store at -20C. Avoid freeze-thaw cycles.
Background: Kallikrein 3/PSA
Alternate Names
KLK3, PSA
Gene Symbol
KLK3
Additional Kallikrein 3/PSA Products
Product Documents for Kallikrein 3/PSA Antibody (RM323)
Certificate of Analysis
To download a Certificate of Analysis, please enter a lot or batch number in the search box below.
Product Specific Notices for Kallikrein 3/PSA Antibody (RM323)
This product is for research use only and is not approved for use in humans or in clinical diagnosis. Primary Antibodies are guaranteed for 1 year from date of receipt.
Customer Reviews for Kallikrein 3/PSA Antibody (RM323)
There are currently no reviews for this product. Be the first to review Kallikrein 3/PSA Antibody (RM323) and earn rewards!
Have you used Kallikrein 3/PSA Antibody (RM323)?
Submit a review and receive an Amazon gift card!
$25/€18/£15/$25CAN/¥2500 Yen for a review with an image
$10/€7/£6/$10CAN/¥1110 Yen for a review without an image
Submit a review
Protocols
Find general support by application which include: protocols, troubleshooting, illustrated assays, videos and webinars.
- Antigen Retrieval Protocol (PIER)
- Antigen Retrieval for Frozen Sections Protocol
- Appropriate Fixation of IHC/ICC Samples
- Cellular Response to Hypoxia Protocols
- Chromogenic IHC Staining of Formalin-Fixed Paraffin-Embedded (FFPE) Tissue Protocol
- Chromogenic Immunohistochemistry Staining of Frozen Tissue
- ClariTSA™ Fluorophore Kits
- Detection & Visualization of Antibody Binding
- ELISA Sample Preparation & Collection Guide
- ELISA Troubleshooting Guide
- Fluorescent IHC Staining of Frozen Tissue Protocol
- Graphic Protocol for Heat-induced Epitope Retrieval
- Graphic Protocol for the Preparation and Fluorescent IHC Staining of Frozen Tissue Sections
- Graphic Protocol for the Preparation and Fluorescent IHC Staining of Paraffin-embedded Tissue Sections
- Graphic Protocol for the Preparation of Gelatin-coated Slides for Histological Tissue Sections
- How to Run an R&D Systems DuoSet ELISA
- How to Run an R&D Systems Quantikine ELISA
- How to Run an R&D Systems Quantikine™ QuicKit™ ELISA
- IHC Sample Preparation (Frozen sections vs Paraffin)
- Immunofluorescent IHC Staining of Formalin-Fixed Paraffin-Embedded (FFPE) Tissue Protocol
- Immunohistochemistry (IHC) and Immunocytochemistry (ICC) Protocols
- Immunohistochemistry Frozen Troubleshooting
- Immunohistochemistry Paraffin Troubleshooting
- Preparing Samples for IHC/ICC Experiments
- Preventing Non-Specific Staining (Non-Specific Binding)
- Primary Antibody Selection & Optimization
- Protocol for Heat-Induced Epitope Retrieval (HIER)
- Protocol for Making a 4% Formaldehyde Solution in PBS
- Protocol for VisUCyte™ HRP Polymer Detection Reagent
- Protocol for the Preparation & Fixation of Cells on Coverslips
- Protocol for the Preparation and Chromogenic IHC Staining of Frozen Tissue Sections
- Protocol for the Preparation and Chromogenic IHC Staining of Frozen Tissue Sections - Graphic
- Protocol for the Preparation and Chromogenic IHC Staining of Paraffin-embedded Tissue Sections
- Protocol for the Preparation and Chromogenic IHC Staining of Paraffin-embedded Tissue Sections - Graphic
- Protocol for the Preparation and Fluorescent IHC Staining of Frozen Tissue Sections
- Protocol for the Preparation and Fluorescent IHC Staining of Paraffin-embedded Tissue Sections
- Protocol for the Preparation of Gelatin-coated Slides for Histological Tissue Sections
- Quantikine HS ELISA Kit Assay Principle, Alkaline Phosphatase
- Quantikine HS ELISA Kit Principle, Streptavidin-HRP Polymer
- R&D Systems Quality Control Western Blot Protocol
- Sandwich ELISA (Colorimetric) – Biotin/Streptavidin Detection Protocol
- Sandwich ELISA (Colorimetric) – Direct Detection Protocol
- TUNEL and Active Caspase-3 Detection by IHC/ICC Protocol
- The Importance of IHC/ICC Controls
- Troubleshooting Guide: ELISA
- Troubleshooting Guide: Immunohistochemistry
- Troubleshooting Guide: Western Blot Figures
- Western Blot Conditions
- Western Blot Protocol
- Western Blot Protocol for Cell Lysates
- Western Blot Troubleshooting
- Western Blot Troubleshooting Guide
- View all Protocols, Troubleshooting, Illustrated assays and Webinars
Loading...