KAT2A/GCN5 Antibody - BSA Free

Novus Biologicals | Catalog # NBP3-37988

Novus Biologicals
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Key Product Details

Species Reactivity

Human, Mouse, Rat

Applications

Immunohistochemistry, Immunohistochemistry-Paraffin, Western Blot, ELISA, Immunocytochemistry/ Immunofluorescence

Label

Unconjugated

Antibody Source

Polyclonal Rabbit IgG

Format

BSA Free
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Product Specifications

Immunogen

Recombinant fusion protein containing a sequence corresponding to amino acids 1-100 of human KAT2A/GCN5 (NP_066564.2).

Sequence:
MAEPSQAPTPAPAAQPRPLQSPAPAPTPTPAPSPASAPIPTPTPAPAPAPAAAPAGSTGTGGPGVGSGGAGSGGDPARPGLSQQQRASQRKAQVRGLPRA

Clonality

Polyclonal

Host

Rabbit

Isotype

IgG

Theoretical MW

94 kDa.
Disclaimer note: The observed molecular weight of the protein may vary from the listed predicted molecular weight due to post translational modifications, post translation cleavages, relative charges, and other experimental factors.

Description

Novus Biologicals Rabbit KAT2A/GCN5 Antibody - BSA Free (NBP3-37988) is a polyclonal antibody validated for use in IHC, WB, ELISA and ICC/IF. All Novus Biologicals antibodies are covered by our 100% guarantee.

Scientific Data Images for KAT2A/GCN5 Antibody - BSA Free

KAT2A/GCN5 Antibody

Immunocytochemistry/ Immunofluorescence: KAT2A/GCN5 Antibody [NBP3-37988] -

Immunocytochemistry/ Immunofluorescence: KAT2A/GCN5 Antibody [NBP3-37988] - Immunofluorescence analysis of C6 cells using KAT2A/GCN5 Rabbit pAb at dilution of 1:100 (40x lens). Secondary antibody: Cy3-conjugated Goat anti-Rabbit IgG (H+L) at 1:500 dilution. Blue: DAPI for nuclear staining.
KAT2A/GCN5 Antibody

Immunocytochemistry/ Immunofluorescence: KAT2A/GCN5 Antibody [NBP3-37988] -

Immunocytochemistry/ Immunofluorescence: KAT2A/GCN5 Antibody [NBP3-37988] - Immunofluorescence analysis of A-549 cells using KAT2A/GCN5 Rabbit pAb at dilution of 1:50 (40x lens). Secondary antibody: Cy3-conjugated Goat anti-Rabbit IgG (H+L) at 1:500 dilution. Blue: DAPI for nuclear staining.
KAT2A/GCN5 Antibody

Immunohistochemistry: KAT2A/GCN5 Antibody [NBP3-37988] -

Immunohistochemistry: KAT2A/GCN5 Antibody [NBP3-37988] - Immunohistochemistry analysis of paraffin-embedded Rat lung using KAT2A/GCN5 Rabbit pAb at dilution of 1:50 (40x lens). High pressure antigen retrieval performed with 0.01M Citrate Bufferr (pH 6.0) prior to IHC staining.
KAT2A/GCN5 Antibody

Immunocytochemistry/ Immunofluorescence: KAT2A/GCN5 Antibody [NBP3-37988] -

Immunocytochemistry/ Immunofluorescence: KAT2A/GCN5 Antibody [NBP3-37988] - Immunofluorescence analysis of L929 cells using KAT2A/GCN5 Rabbit pAb at dilution of 1:100 (40x lens). Secondary antibody: Cy3-conjugated Goat anti-Rabbit IgG (H+L) at 1:500 dilution. Blue: DAPI for nuclear staining.
KAT2A/GCN5 Antibody

Immunohistochemistry: KAT2A/GCN5 Antibody [NBP3-37988] -

Immunohistochemistry: KAT2A/GCN5 Antibody [NBP3-37988] - Immunohistochemistry analysis of paraffin-embedded Mouse brain using KAT2A/GCN5 Rabbit pAb at dilution of 1:50 (40x lens). High pressure antigen retrieval performed with 0.01M Citrate Bufferr (pH 6.0) prior to IHC staining.
KAT2A/GCN5 Antibody

Immunohistochemistry: KAT2A/GCN5 Antibody [NBP3-37988] -

Immunohistochemistry: KAT2A/GCN5 Antibody [NBP3-37988] - Immunohistochemistry analysis of paraffin-embedded Mouse heart using KAT2A/GCN5 Rabbit pAb at dilution of 1:50 (40x lens). High pressure antigen retrieval performed with 0.01M Citrate Bufferr (pH 6.0) prior to IHC staining.
KAT2A/GCN5 Antibody

Immunocytochemistry/ Immunofluorescence: KAT2A/GCN5 Antibody [NBP3-37988] -

Immunocytochemistry/ Immunofluorescence: KAT2A/GCN5 Antibody [NBP3-37988] - Immunofluorescence analysis of U-2 OS cells using KAT2A/GCN5 Rabbit pAb at dilution of 1:100 (40x lens). Secondary antibody: Cy3-conjugated Goat anti-Rabbit IgG (H+L) at 1:500 dilution. Blue: DAPI for nuclear staining.
KAT2A/GCN5 Antibody

Western Blot: KAT2A/GCN5 Antibody [NBP3-37988] -

Western Blot: KAT2A/GCN5 Antibody [NBP3-37988] - Western blot analysis of lysates from Rat brain, using KAT2A/GCN5 Rabbit pAb at 1:400 dilution.
Secondary antibody: HRP-conjugated Goat anti-Rabbit IgG (H+L) at 1:10000 dilution.
Lysates/proteins: 25ug per lane.
Blocking buffer: 3% nonfat dry milk in TBST.
Detection: ECL Basic Kit.
Exposure time: 30s.

Applications for KAT2A/GCN5 Antibody - BSA Free

Application
Recommended Usage

Immunocytochemistry/ Immunofluorescence

1:50 - 1:200

Immunohistochemistry-Paraffin

1:50 - 1:200

Western Blot

1:100 - 1:500

Formulation, Preparation, and Storage

Purification

Affinity purified

Formulation

PBS (pH 7.3), 50% glycerol

Format

BSA Free

Preservative

0.02% Sodium Azide

Concentration

Please see the vial label for concentration. If unlisted please contact technical services.

Shipping

The product is shipped with polar packs. Upon receipt, store it immediately at the temperature recommended below.

Stability & Storage

Store at -20C. Avoid freeze-thaw cycles.

Background: KAT2A/GCN5

KAT2A, or GCN5, is a histone acetyltransferase (HAT) that functions primarily as a transcriptional activator. It alsofunctions as a repressor of NF-kappa-B (see MIM 164011) by promoting ubiquitination of the NF-kappa-B subunit RELA(MIM 164014) in a HAT-independent manner (Mao et al., 2009 (PubMed 19339690)).(supplied by OMIM)

Long Name

Lysine Acetyltransferase 2A

Alternate Names

GCN5L2, HGCN5, KAT2A, PCAF-B

Gene Symbol

KAT2A

Additional KAT2A/GCN5 Products

Product Documents for KAT2A/GCN5 Antibody - BSA Free

Certificate of Analysis

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Product Specific Notices for KAT2A/GCN5 Antibody - BSA Free

This product is for research use only and is not approved for use in humans or in clinical diagnosis. Primary Antibodies are guaranteed for 1 year from date of receipt.

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Protocols

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FAQs

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