KCC3/SLC12A6 Antibody
Novus Biologicals | Catalog # NBP1-06044
![Western Blot: KCC3/SLC12A6 Antibody [NBP1-06044]](https://resources.rndsystems.com/images/products/KCC3-SLC12A6-Antibody-Western-Blot-NBP1-06044-img0005.jpg "Western Blot: KCC3/SLC12A6 Antibody [NBP1-06044]")
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Key Product Details
Species Reactivity
Human
Applications
Immunohistochemistry, Immunohistochemistry-Paraffin, Western Blot, Peptide ELISA
Label
Unconjugated
Antibody Source
Polyclonal Goat IgG
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Product Specifications
Immunogen
Peptide with sequence SRHIDVCSKTKE corresponding to internal region according to NP_001035959.1, NP_001035960.1, NP_001035961.1, NP_001035962.1, NP_005126.1, NP_598408.1.
Epitope
SRHIDVCSKTKE
Specificity
This antibody is expected to recognise all 6 reported isofoms (NP_598408.1; NP_005126.1; NP_001035959.1; NP_001035960.1; NP_001035961.1; NP_001035962.1; );
Clonality
Polyclonal
Host
Goat
Isotype
IgG
Description
Novus Biologicals Goat KCC3/SLC12A6 Antibody (NBP1-06044) is a polyclonal antibody validated for use in IHC, WB and ELISA. Anti-KCC3/SLC12A6 Antibody: Cited in 2 publications. All Novus Biologicals antibodies are covered by our 100% guarantee.
Scientific Data Images for KCC3/SLC12A6 Antibody
Western Blot: KCC3/SLC12A6 Antibody [NBP1-06044]
Western Blot: KCC3/SLC12A6 Antibody [NBP1-06044] - (0.1ug/ml) staining of Human Heart lysate (35ug protein in RIPA buffer). Primary incubation was 1 hour. Detected by chemiluminescence.![Immunohistochemistry-Paraffin: KCC3/SLC12A6 Antibody [NBP1-06044]](https://resources.rndsystems.com/images/products/KCC3-SLC12A6-Antibody-Immunohistochemistry-Paraffin-NBP1-06044-img0004.jpg "Immunohistochemistry-Paraffin: KCC3/SLC12A6 Antibody [NBP1-06044]")
Immunohistochemistry-Paraffin: KCC3/SLC12A6 Antibody [NBP1-06044]
Immunohistochemistry-Paraffin: KCC3/SLC12A6 Antibody [NBP1-06044] - (2.5ug/ml) staining of paraffin embedded Human Heart. Steamed antigen retrieval with citrate buffer pH 6, AP-staining.Applications for KCC3/SLC12A6 Antibody
Application
Recommended Usage
Immunohistochemistry
2 - 4 ug/ml
Immunohistochemistry-Paraffin
2 - 4 ug/ml
Peptide ELISA
Detection limit 1:16000
Western Blot
0.1 - 0.3 ug/ml
Application Notes
WB: Approx. 120 kDa band observed in human heart and kidney lysates (calculated MW ranging 121-128 kDa band according to NP_598408.1; NP_005126.1; NP_001035959.1; NP_001035960.1; NP_001035961.1; NP_001035962.1). IHC-P: Human heart shows staining of myocardial fibrils in transverse section.
Formulation, Preparation, and Storage
Purification
Immunogen affinity purified
Formulation
Tris saline (20 mM Tris pH 7.3, 150 mM NaCl), 0.5% BSA
Preservative
0.02% Sodium Azide
Concentration
0.5 mg/ml
Shipping
The product is shipped with polar packs. Upon receipt, store it immediately at the temperature recommended below.
Stability & Storage
Store at -20C. Avoid freeze-thaw cycles.
Background: KCC3/SLC12A6
Long Name
K-Cl Cotransporter 3
Alternate Names
ACCPN, SLC12A6
Entrez Gene IDs
9990 (Human)
Gene Symbol
SLC12A6
UniProt
Additional KCC3/SLC12A6 Products
Product Documents for KCC3/SLC12A6 Antibody
Certificate of Analysis
To download a Certificate of Analysis, please enter a lot or batch number in the search box below.
Product Specific Notices for KCC3/SLC12A6 Antibody
This product is for research use only and is not approved for use in humans or in clinical diagnosis. Primary Antibodies are guaranteed for 1 year from date of receipt.
Related Research Areas
Citations for KCC3/SLC12A6 Antibody
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Protocols
Find general support by application which include: protocols, troubleshooting, illustrated assays, videos and webinars.
- Antigen Retrieval Protocol (PIER)
- Antigen Retrieval for Frozen Sections Protocol
- Appropriate Fixation of IHC/ICC Samples
- Cellular Response to Hypoxia Protocols
- Chromogenic IHC Staining of Formalin-Fixed Paraffin-Embedded (FFPE) Tissue Protocol
- Chromogenic Immunohistochemistry Staining of Frozen Tissue
- Detection & Visualization of Antibody Binding
- ELISA Sample Preparation & Collection Guide
- ELISA Troubleshooting Guide
- Fluorescent IHC Staining of Frozen Tissue Protocol
- Graphic Protocol for Heat-induced Epitope Retrieval
- Graphic Protocol for the Preparation and Fluorescent IHC Staining of Frozen Tissue Sections
- Graphic Protocol for the Preparation and Fluorescent IHC Staining of Paraffin-embedded Tissue Sections
- Graphic Protocol for the Preparation of Gelatin-coated Slides for Histological Tissue Sections
- How to Run an R&D Systems DuoSet ELISA
- How to Run an R&D Systems Quantikine ELISA
- How to Run an R&D Systems Quantikine™ QuicKit™ ELISA
- IHC Sample Preparation (Frozen sections vs Paraffin)
- Immunofluorescent IHC Staining of Formalin-Fixed Paraffin-Embedded (FFPE) Tissue Protocol
- Immunohistochemistry (IHC) and Immunocytochemistry (ICC) Protocols
- Immunohistochemistry Frozen Troubleshooting
- Immunohistochemistry Paraffin Troubleshooting
- Preparing Samples for IHC/ICC Experiments
- Preventing Non-Specific Staining (Non-Specific Binding)
- Primary Antibody Selection & Optimization
- Protocol for Heat-Induced Epitope Retrieval (HIER)
- Protocol for Making a 4% Formaldehyde Solution in PBS
- Protocol for VisUCyte™ HRP Polymer Detection Reagent
- Protocol for the Preparation & Fixation of Cells on Coverslips
- Protocol for the Preparation and Chromogenic IHC Staining of Frozen Tissue Sections
- Protocol for the Preparation and Chromogenic IHC Staining of Frozen Tissue Sections - Graphic
- Protocol for the Preparation and Chromogenic IHC Staining of Paraffin-embedded Tissue Sections
- Protocol for the Preparation and Chromogenic IHC Staining of Paraffin-embedded Tissue Sections - Graphic
- Protocol for the Preparation and Fluorescent IHC Staining of Frozen Tissue Sections
- Protocol for the Preparation and Fluorescent IHC Staining of Paraffin-embedded Tissue Sections
- Protocol for the Preparation of Gelatin-coated Slides for Histological Tissue Sections
- Quantikine HS ELISA Kit Assay Principle, Alkaline Phosphatase
- Quantikine HS ELISA Kit Principle, Streptavidin-HRP Polymer
- R&D Systems Quality Control Western Blot Protocol
- Sandwich ELISA (Colorimetric) – Biotin/Streptavidin Detection Protocol
- Sandwich ELISA (Colorimetric) – Direct Detection Protocol
- TUNEL and Active Caspase-3 Detection by IHC/ICC Protocol
- The Importance of IHC/ICC Controls
- Troubleshooting Guide: ELISA
- Troubleshooting Guide: Immunohistochemistry
- Troubleshooting Guide: Western Blot Figures
- Western Blot Conditions
- Western Blot Protocol
- Western Blot Protocol for Cell Lysates
- Western Blot Troubleshooting
- Western Blot Troubleshooting Guide
- View all Protocols, Troubleshooting, Illustrated assays and Webinars
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