KCNJ10 Antibody

Novus Biologicals | Catalog # NBP1-20149

Novus Biologicals
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Key Product Details

Species Reactivity

Validated:

Human, Mouse, Rat

Cited:

Human

Applications

Validated:

Immunohistochemistry, Immunohistochemistry-Paraffin, Immunohistochemistry-Frozen, Western Blot

Cited:

Western Blot

Label

Unconjugated

Antibody Source

Polyclonal Rabbit IgG
View all KCNJ10 Primary Antibodies »

Product Specifications

Immunogen

A synthetic peptide from amino acid region 330-379 of human KCNJ10 conjugated to blue carrier protein was used as the antigen. The antigen is homologous in rat and mouse.

Clonality

Polyclonal

Host

Rabbit

Isotype

IgG

Scientific Data Images for KCNJ10 Antibody

Western Blot: KCNJ10 Antibody [NBP1-20149]

Western Blot: KCNJ10 Antibody [NBP1-20149]

Western Blot: KCNJ10 Antibody [NBP1-20149] - WB on mouse tissue lysate. Blocking: 1% LFDM for 30 min at RT; primary antibody: dilution 1:3000 incubated at 4C overnight.
Immunohistochemistry-Paraffin: KCNJ10 Antibody [NBP1-20149]

Immunohistochemistry-Paraffin: KCNJ10 Antibody [NBP1-20149]

Immunohistochemistry-Paraffin: KCNJ10 Antibody [NBP1-20149] - IHC-P on paraffin sections of rat brain. The animal was perfused using Autoperfuser at a pressure of 130 mmHg with 300 ml 4% FA before being processed for paraffin embedding. HIER: Tris-EDTA, pH 9 for 20 min using Thermo PT Module. Blocking: 0.2% LFDM in TBST filtered thru 0.2 um. Detection was done using HRP polymer following manufacturers instructions; DAB chromogen: Candela DAB chromogen from Osenses. Primary antibody: dilution 1: 2000, incubated 30 min at RT using Autostainer. Sections were counterstained with Harris Hematoxylin.
Immunohistochemistry-Paraffin: KCNJ10 Antibody [NBP1-20149]

Immunohistochemistry-Paraffin: KCNJ10 Antibody [NBP1-20149]

Immunohistochemistry-Paraffin: KCNJ10 Antibody [NBP1-20149] - IHC-P on paraffin sections of rat brain. The animal was perfused at a pressure of 130 mmHg with 300 ml 4% FA before being processed for paraffin embedding. HIER: Tris-EDTA, pH 9 for 20 min. Blocking: 0.2% LFDM in TBST filtered thru 0.2 um. Detection was done using HRP polymer following manufacturers instructions; DAB chromogen. Primary antibody: dilution 1: 2000, incubated 30 min at RT. Sections were counterstained with Harris Hematoxylin.
Immunohistochemistry-Paraffin: KCNJ10 Antibody [NBP1-20149]

Immunohistochemistry-Paraffin: KCNJ10 Antibody [NBP1-20149]

Immunohistochemistry-Paraffin: KCNJ10 Antibody [NBP1-20149] - IHC-P on paraffin sections of rat brain. The animal was perfused at a pressure of 130 mmHg with 300 ml 4% FA before being processed for paraffin embedding. HIER: Tris-EDTA, pH 9 for 20 min. Blocking: 0.2% LFDM in TBST filtered thru 0.2 um. Detection was done using HRP polymer following manufacturers instructions; DAB chromogen. Primary antibody: dilution 1: 2000, incubated 30 min at RT. Sections were counterstained with Harris Hematoxylin.
Immunohistochemistry-Paraffin: KCNJ10 Antibody [NBP1-20149]

Immunohistochemistry-Paraffin: KCNJ10 Antibody [NBP1-20149]

Immunohistochemistry-Paraffin: KCNJ10 Antibody [NBP1-20149] - IHC-P on paraffin sections of mouse kidney. The animal was perfused at a pressure of 130 mmHg with 300 ml 4% FA before being processed for paraffin embedding. HIER: Tris-EDTA, pH 9 for 20 min. Blocking: 0.2% LFDM in TBST filtered thru 0.2 um. Detection was done using HRP polymer from following manufacturers instructions; DAB chromogen. Primary antibody: dilution 1: 2000, incubated 30 min at RT. Sections were counterstained with Harris Hematoxylin.
Immunohistochemistry-Paraffin: KCNJ10 Antibody [NBP1-20149]

Immunohistochemistry-Paraffin: KCNJ10 Antibody [NBP1-20149]

Immunohistochemistry-Paraffin: KCNJ10 Antibody [NBP1-20149] - IHC-P on paraffin sections of rat kidney. The animal was perfused at a pressure of 130 mmHg with 300 ml 4% FA before being processed for paraffin embedding. HIER: Tris-EDTA, pH 9 for 20 min. Blocking: 0.2% LFDM in TBST filtered thru 0.2 um. Detection was done using HRP polymer following manufacturers instructions; DAB chromogen. Primary antibody: dilution 1: 2000, incubated 30 min at RT. Sections were counterstained with Harris Hematoxylin.
Immunohistochemistry-Paraffin: KCNJ10 Antibody [NBP1-20149]

Immunohistochemistry-Paraffin: KCNJ10 Antibody [NBP1-20149]

Immunohistochemistry-Paraffin: KCNJ10 Antibody [NBP1-20149] - IHC-P on paraffin sections of rat kidney. The animal was perfused at a pressure of 130 mmHg with 300 ml 4% FA before being processed for paraffin embedding. HIER: Tris-EDTA, pH 9 for 20 min. Blocking: 0.2% LFDM in TBST filtered thru 0.2 um. Detection was done using HRP polymer following manufacturers instructions; DAB chromogen. Primary antibody: dilution 1: 2000, incubated 30 min at RT. Sections were counterstained with Harris Hematoxylin.
Immunohistochemistry-Paraffin: KCNJ10 Antibody [NBP1-20149]

Immunohistochemistry-Paraffin: KCNJ10 Antibody [NBP1-20149]

Immunohistochemistry-Paraffin: KCNJ10 Antibody [NBP1-20149] - IHC-P on paraffin sections of rat heart. The animal was perfused at a pressure of 130 mmHg with 300 ml 4% FA before being processed for paraffin embedding. HIER: Tris-EDTA, pH 9 for 20 min. Blocking: 0.2% LFDM in TBST filtered thru 0.2 um. Detection was done using HRP polymer following manufacturers instructions; DAB chromogen. Primary antibody: dilution 1: 2000, incubated 30 min at RT. Sections were counterstained with Harris Hematoxylin.
Immunohistochemistry-Paraffin: KCNJ10 Antibody [NBP1-20149]

Immunohistochemistry-Paraffin: KCNJ10 Antibody [NBP1-20149]

Immunohistochemistry-Paraffin: KCNJ10 Antibody [NBP1-20149] - IHC-P on paraffin sections of rat brain. The animal was perfused using Autoperfuser at a pressure of 130 mmHg with 300 ml 4% FA before being processed for paraffin embedding. HIER: Tris-EDTA, pH 9 for 20 min using Thermo PT Module. Blocking: 0.2% LFDM in TBST filtered thru 0.2 um. Detection was done using HRP polymer following manufacturers instructions; DAB chromogen: Candela DAB chromogen from Osenses. Primary antibody: dilution 1: 2000, incubated 30 min at RT using Autostainer. Sections were counterstained with Harris Hematoxylin.

Applications for KCNJ10 Antibody

Application
Recommended Usage

Immunohistochemistry

1:10-1:500

Immunohistochemistry-Frozen

1:10-1:500

Immunohistochemistry-Paraffin

1:2000

Western Blot

1:3000

Formulation, Preparation, and Storage

Purification

Unpurified

Reconstitution

Reconstitute in 0.1 ml of sterile water. Centrifuge to remove any insoluble material. Glycerol may be added (1:1) for additional stability. Please note the sample size is provided in reconstituted format.

Formulation

Lyophilized from whole antisera

Preservative

No Preservative

Concentration

This product is unpurified. The exact concentration of antibody is not quantifiable.

Shipping

The product is shipped with polar packs. Upon receipt, store it immediately at the temperature recommended below.

Stability & Storage

Store at 4C short term. Aliquot and store at -20C long term. Avoid freeze-thaw cycles.

Calculators

The reconstitution calculator allows you to quickly calculate the volume of a reagent to reconstitute your vial. Simply enter the mass of reagent and the target concentration and the calculator will determine the rest.

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Background: KCNJ10

FUNCTION: May be responsible for potassium buffering action of glial cells in the brain. Inward rectifier potassium channels are characterized by a greater tendency to allow potassium to flow into the cell rather than out of it. Their voltage dependence is regulated by the concentration of extracellular potassium; as external potassium is raised, the voltage range of the channel opening shifts to more positive voltages. The inward rectification is mainly due to the blockage of outward current by internal magnesium. Can be blocked by extracellular barium and cesium.; Subcellular location: Membrane, Multi-pass membrane protein.

Long Name

ATP-sensitive inward rectifier potassium channel 10

Alternate Names

ATP-dependent inwardly rectifying potassium channel Kir4.1, ATP-sensitive inward rectifier potassium channel 10, BIRK-10, glial ATP-dependent inwardly rectifying potassium channel KIR4.1, Inward rectifier K(+) channel Kir1.2, inward rectifier K+ channel KIR1.2, KCNJ13-PEN, Kir1.2, KIR4.1, Potassium channel, inwardly rectifying subfamily J member 10, potassium inwardly-rectifying channel, subfamily J, member 10, SESAME

Entrez Gene IDs

3766 (Human); 16513 (Mouse); 29718 (Rat)

Gene Symbol

KCNJ10

UniProt

P78508 (Human)

Additional KCNJ10 Products

Product Documents for KCNJ10 Antibody

Certificate of Analysis

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Product Specific Notices for KCNJ10 Antibody

This product is for research use only and is not approved for use in humans or in clinical diagnosis. Primary Antibodies are guaranteed for 1 year from date of receipt.

Citations for KCNJ10 Antibody

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Protocols

Find general support by application which include: protocols, troubleshooting, illustrated assays, videos and webinars.

FAQs

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