KLF17 Antibody - BSA Free

Novus Biologicals | Catalog # NBP1-81917

Novus Biologicals
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Key Product Details

Validated by

Orthogonal Validation

Species Reactivity

Validated:

Human

Cited:

Human

Applications

Validated:

Immunohistochemistry, Immunohistochemistry-Paraffin, Western Blot, Simple Western

Cited:

IF/IHC

Label

Unconjugated

Antibody Source

Polyclonal Rabbit IgG

Format

BSA Free
View all KLF17 Primary Antibodies »

Product Specifications

Immunogen

This antibody was developed against Recombinant Protein corresponding to amino acids: TVPSTEAQAVLPSMAQMLPPQDAHDLGMPPAESQSLLVLGSQDSLVSQPDSQEGPFLPEQPGPAPQTVEKNSRPQEGTG

Reactivity Notes

Human reactivity reported in scientific literature (PMID: 26293300).

Clonality

Polyclonal

Host

Rabbit

Isotype

IgG

Scientific Data Images for KLF17 Antibody - BSA Free

Immunohistochemistry-Paraffin: KLF17 Antibody [NBP1-81917]

Immunohistochemistry-Paraffin: KLF17 Antibody [NBP1-81917]

Immunohistochemistry-Paraffin: KLF17 Antibody [NBP1-81917] - Analysis in human testis and tonsil tissues. Corresponding KLF17 RNA-seq data are presented for the same tissues.
Western Blot: KLF17 Antibody [NBP1-81917]

Western Blot: KLF17 Antibody [NBP1-81917]

Western Blot: KLF17 Antibody [NBP1-81917] - Lane 1: Marker [kDa] 250, 130, 95, 72, 55, 36, 28, 17, 10. Lane 2: Negative control (vector only transfected HEK293T lysate). Lane 3: Over-expression lysate (Co-expressed with a C-terminal myc-DDK tag (3.1 kDa) in mammalian HEK293T cells).
Immunohistochemistry-Paraffin: KLF17 Antibody [NBP1-81917]

Immunohistochemistry-Paraffin: KLF17 Antibody [NBP1-81917]

Immunohistochemistry-Paraffin: KLF17 Antibody [NBP1-81917] - Staining of human tonsil shows low expression as expected.
Immunohistochemistry-Paraffin: KLF17 Antibody [NBP1-81917]

Immunohistochemistry-Paraffin: KLF17 Antibody [NBP1-81917]

Immunohistochemistry-Paraffin: KLF17 Antibody [NBP1-81917] - Staining of human pancreas shows no nuclear positivity in exocrine glandular cells as expected.
Immunohistochemistry-Paraffin: KLF17 Antibody [NBP1-81917]

Immunohistochemistry-Paraffin: KLF17 Antibody [NBP1-81917]

Immunohistochemistry-Paraffin: KLF17 Antibody [NBP1-81917] - Staining of human skeletal muscle shows no nuclear positivity in myocytes as expected.
Immunohistochemistry-Paraffin: KLF17 Antibody [NBP1-81917]

Immunohistochemistry-Paraffin: KLF17 Antibody [NBP1-81917]

Immunohistochemistry-Paraffin: KLF17 Antibody [NBP1-81917] - Staining of human testis shows strong nuclear positivity in spermatids.

Applications for KLF17 Antibody - BSA Free

Application
Recommended Usage

Immunohistochemistry

1:200 - 1:500

Immunohistochemistry-Paraffin

1:200 - 1:500

Simple Western

1:500

Western Blot

0.04-0.4 ug/ml
Application Notes
IHC reported in scientific literature (PMID: 26293300). For IHC-Paraffin, HIER pH 6 retrieval is recommended.

Formulation, Preparation, and Storage

Purification

Affinity purified

Formulation

PBS (pH 7.2) and 40% Glycerol

Format

BSA Free

Preservative

0.02% Sodium Azide

Concentration

Concentrations vary lot to lot. See vial label for concentration. If unlisted please contact technical services.

Shipping

The product is shipped with polar packs. Upon receipt, store it immediately at the temperature recommended below.

Stability & Storage

Store at 4C short term. Aliquot and store at -20C long term. Avoid freeze-thaw cycles.

Background: KLF17

Transcription repressor that binds to the promoter of target genes and prevents their expression. Acts as anegative regulator of epithelial-mesenchymal transition and metastasis in breast cancer. Specifically binds the5'-CACCC-3' sequence in the promoter of ID1, a key metastasis regulator in breast cancer, and repress its expression.May be a germ cell-specific transcription factor that plays important roles in spermatid differentiation and oocytedevelopment

Long Name

Kruppel-Like Factor 17

Alternate Names

Zfp393, ZNF393

Gene Symbol

KLF17

Additional KLF17 Products

Product Documents for KLF17 Antibody - BSA Free

Certificate of Analysis

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Product Specific Notices for KLF17 Antibody - BSA Free

This product is for research use only and is not approved for use in humans or in clinical diagnosis. Primary Antibodies are guaranteed for 1 year from date of receipt.

Citations for KLF17 Antibody - BSA Free

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Protocols

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