Key Product Details

Species Reactivity

All Species

Applications

Western Blot, ELISA

Label

Alkaline Phosphatase

Antibody Source

Polyclonal Rabbit IgG
View all KLH Primary Antibodies »

Product Specifications

Immunogen

KLH (Keyhole Limpet Hemocyanin)

Localization

Secreted protein; extracellular space

Clonality

Polyclonal

Host

Rabbit

Isotype

IgG

Description

Store vial at 4C before opening. DO NOT FREEZE. This product is stable at 4C as an undiluted liquid. Dilute only prior to immediate use. Freezing alkaline phosphatase conjugates will result in a substantial loss of enzymatic activity.

Stabilizer: 10 mg/ml Bovine Serum Albumin (BSA)-Immunoglobulin and Protease free
Color: Substrate dependent
Conjugation Chemistry: Glutaraldehye coupling

This product was prepared from monospecific antiserum by immunoaffinity chromatography using KLH coupled to agarose beads followed by solid phase adsorption(s) to remove any unwanted reactivities. Assay by immunoelectrophoresis resulted in a single precipitin arc against anti-Alkaline Phosphatase (calf intestine), anti-Rabbit Serum and KLH.

Scientific Data Images for KLH Antibody [Alkaline Phosphatase]

Western Blot: KLH Antibody [Alkaline Phosphatase] [NBP1-97336] - Lane 1: molecular weight. Lane 2: reduced KLH. Load: 1 ug. Primary antibody: KLH antibody at 1:1000 for overnight at 4C. Predicted/Observed size: 350-390 kDa, 350-390 kDa for KLH. Other band(s): none.

Applications for KLH Antibody [Alkaline Phosphatase]

Application
Recommended Usage

ELISA

1:15000-1:60000

Western Blot

1:100-1:2000
Application Notes
This product has been assayed against 1.0 ug of KLH in a standard capture ELISA using pNPP p-nitrophenyl phosphate as a substrate for 30 minutes at room temperature. A working dilution of 1:3,000 to 1:13,500 is suggested for this product.

Formulation, Preparation, and Storage

Purification

Immunogen affinity purified

Formulation

0.05 M Tris Chloride, 0.15M Sodium Chloride, 0.001M Magnesium Chloride, 0.0001M Zinc Chloride, 50% (v/v) Glycerol; pH 8.0, 10 mg/mL Bovine Serum Albumin (BSA) - Immunoglobulin and Protease free

Preservative

0.01% Sodium Azide

Concentration

Please see the vial label for concentration. If unlisted please contact technical services.

Shipping

The product is shipped with polar packs. Upon receipt, store it immediately at the temperature recommended below.

Stability & Storage

Store at 4C in the dark. Do not freeze.

Background: KLH

KLH (Keyhole Limpet Hemocyanin) is a copper containing oxygen carrier occurring freely dissolved in the hemolymph of many mollusks and arthropods. KLH is often used in molecular immunology as a carrier protein conjugated to low molecular weight molecules such as peptides, amino acids, nucleic acids, drugs or toxins to render them more immunogenic due to the size of the conjugate complex and the immunogenicity of KLH. KLH forms a large complex composed of ~50 kDa subunits.

Alternate Names

keyhole limpet hemocyanin (KLH)

Additional KLH Products

Product Documents for KLH Antibody [Alkaline Phosphatase]

Certificate of Analysis

To download a Certificate of Analysis, please enter a lot or batch number in the search box below.

Download SDS

Product Specific Notices for KLH Antibody [Alkaline Phosphatase]

This product is for research use only and is not approved for use in humans or in clinical diagnosis. Primary Antibodies are guaranteed for 1 year from date of receipt.

Customer Reviews for KLH Antibody [Alkaline Phosphatase]

There are currently no reviews for this product. Be the first to review KLH Antibody [Alkaline Phosphatase] and earn rewards!

Have you used KLH Antibody [Alkaline Phosphatase]?

Submit a review and receive an Amazon gift card!

$25/€18/£15/$25CAN/¥2500 Yen for a review with an image

$10/€7/£6/$10CAN/¥1110 Yen for a review without an image

Submit a review
Amazon Gift Card

FAQs

No product specific FAQs exist for this product.

View all FAQs for Antibodies