Klotho Antibody
Novus Biologicals | Catalog # NB100-2432
![Immunohistochemistry-Paraffin: Klotho Antibody [NB100-2432]](https://beta-resources.rndsystems.com/images/products/Klotho-Antibody-Immunohistochemistry-Paraffin-NB100-2432-img0007.jpg "Immunohistochemistry-Paraffin: Klotho Antibody [NB100-2432]")
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Key Product Details
Species Reactivity
Validated:
Human
Predicted:
Mouse (100%), Rat (100%). Backed by our 100% Guarantee.
Applications
Immunohistochemistry, Immunohistochemistry-Paraffin, Peptide ELISA
Label
Unconjugated
Antibody Source
Polyclonal Goat IgG
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Product Specifications
Immunogen
Peptide with sequence C-KRDDAKYMYYLKK corresponding to internal region according to NP_004786.2, NP_710150.1.
Specificity
This antibody is expected to recognise both reported isoforms (NP_004786.2; NP_710150.1).
Clonality
Polyclonal
Host
Goat
Isotype
IgG
Description
Novus Biologicals Goat Klotho Antibody (NB100-2432) is a polyclonal antibody validated for use in IHC and ELISA. Anti-Klotho Antibody: Cited in 1 publication. All Novus Biologicals antibodies are covered by our 100% guarantee.
Scientific Data Images for Klotho Antibody
Immunohistochemistry-Paraffin: Klotho Antibody [NB100-2432]
Immunohistochemistry-Paraffin: Klotho Antibody [NB100-2432] - Staining of Human Placenta. Steamed antigen retrieval with citrate buffer pH 6, HRP-staining.![Immunohistochemistry-Paraffin: Klotho Antibody [NB100-2432]](https://beta-resources.rndsystems.com/images/products/Klotho-Antibody-Immunohistochemistry-Paraffin-NB100-2432-img0005.jpg "Immunohistochemistry-Paraffin: Klotho Antibody [NB100-2432]")
Immunohistochemistry-Paraffin: Klotho Antibody [NB100-2432]
Immunohistochemistry-Paraffin: Klotho Antibody [NB100-2432] - Staining of Human Breast. Steamed antigen retrieval with citrate buffer pH 6, HRP-staining.Applications for Klotho Antibody
Application
Recommended Usage
Immunohistochemistry
4 - 6 ug/ml
Immunohistochemistry-Paraffin
4 - 6 ug/ml
Peptide ELISA
Detection limit 1:4000
Application Notes
WB: Preliminary experiments in human brain, duodenum, placenta and prostate lysates gave no specific signal but low background. IHC-P: Human breast shows cytoplasm staining in luminal epithelial cells of ducts, while human placenta shows membranous staining.
Formulation, Preparation, and Storage
Purification
Immunogen affinity purified
Formulation
Tris saline (20 mM Tris pH 7.3, 150 mM NaCl), 0.5% BSA
Preservative
0.02% Sodium Azide
Concentration
0.5 mg/ml
Shipping
The product is shipped with polar packs. Upon receipt, store it immediately at the temperature recommended below.
Stability & Storage
Store at -20C. Avoid freeze-thaw cycles.
Background: Klotho
Additional Klotho Products
Product Documents for Klotho Antibody
Product Specific Notices for Klotho Antibody
This product is for research use only and is not approved for use in humans or in clinical diagnosis. Primary Antibodies are guaranteed for 1 year from date of receipt.
Related Research Areas
Citations for Klotho Antibody
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Protocols
Find general support by application which include: protocols, troubleshooting, illustrated assays, videos and webinars.
- Antigen Retrieval Protocol (PIER)
- Antigen Retrieval for Frozen Sections Protocol
- Appropriate Fixation of IHC/ICC Samples
- Cellular Response to Hypoxia Protocols
- Chromogenic IHC Staining of Formalin-Fixed Paraffin-Embedded (FFPE) Tissue Protocol
- Chromogenic Immunohistochemistry Staining of Frozen Tissue
- Detection & Visualization of Antibody Binding
- ELISA Sample Preparation & Collection Guide
- ELISA Troubleshooting Guide
- Fluorescent IHC Staining of Frozen Tissue Protocol
- Graphic Protocol for Heat-induced Epitope Retrieval
- Graphic Protocol for the Preparation and Fluorescent IHC Staining of Frozen Tissue Sections
- Graphic Protocol for the Preparation and Fluorescent IHC Staining of Paraffin-embedded Tissue Sections
- Graphic Protocol for the Preparation of Gelatin-coated Slides for Histological Tissue Sections
- How to Run an R&D Systems DuoSet ELISA
- How to Run an R&D Systems Quantikine ELISA
- How to Run an R&D Systems Quantikine™ QuicKit™ ELISA
- IHC Sample Preparation (Frozen sections vs Paraffin)
- Immunofluorescent IHC Staining of Formalin-Fixed Paraffin-Embedded (FFPE) Tissue Protocol
- Immunohistochemistry (IHC) and Immunocytochemistry (ICC) Protocols
- Immunohistochemistry Frozen Troubleshooting
- Immunohistochemistry Paraffin Troubleshooting
- Preparing Samples for IHC/ICC Experiments
- Preventing Non-Specific Staining (Non-Specific Binding)
- Primary Antibody Selection & Optimization
- Protocol for Heat-Induced Epitope Retrieval (HIER)
- Protocol for Making a 4% Formaldehyde Solution in PBS
- Protocol for VisUCyte™ HRP Polymer Detection Reagent
- Protocol for the Preparation & Fixation of Cells on Coverslips
- Protocol for the Preparation and Chromogenic IHC Staining of Frozen Tissue Sections
- Protocol for the Preparation and Chromogenic IHC Staining of Frozen Tissue Sections - Graphic
- Protocol for the Preparation and Chromogenic IHC Staining of Paraffin-embedded Tissue Sections
- Protocol for the Preparation and Chromogenic IHC Staining of Paraffin-embedded Tissue Sections - Graphic
- Protocol for the Preparation and Fluorescent IHC Staining of Frozen Tissue Sections
- Protocol for the Preparation and Fluorescent IHC Staining of Paraffin-embedded Tissue Sections
- Protocol for the Preparation of Gelatin-coated Slides for Histological Tissue Sections
- Quantikine HS ELISA Kit Assay Principle, Alkaline Phosphatase
- Quantikine HS ELISA Kit Principle, Streptavidin-HRP Polymer
- Sandwich ELISA (Colorimetric) – Biotin/Streptavidin Detection Protocol
- Sandwich ELISA (Colorimetric) – Direct Detection Protocol
- TUNEL and Active Caspase-3 Detection by IHC/ICC Protocol
- The Importance of IHC/ICC Controls
- Troubleshooting Guide: ELISA
- Troubleshooting Guide: Immunohistochemistry
- View all Protocols, Troubleshooting, Illustrated assays and Webinars
FAQs for Klotho Antibody
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A: A list of our klotho products can be found here. I am not entirely sure what you are looking for as I don't have your complete message for some reason. Can you please clarify what you are looking for?
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Q: Upon the customer's inquiry, may we ask if there is any product specific reference available for NB100-2432?
A: For all of our products if there is not a reference listed on the data sheet under the product specific reference section we have not found one at this time and do not have one to provide. We are always updating our references as we come across them and will display them on the website if it is available.
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