KMT2A/MLL Antibody - BSA Free

Novus Biologicals | Catalog # NB600-248

Novus Biologicals
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Key Product Details

Validated by

Biological Validation

Species Reactivity

Validated:

Human, Mouse

Cited:

Human, Mouse

Applications

Validated:

Immunohistochemistry, Immunohistochemistry-Paraffin, Western Blot, Immunoprecipitation

Cited:

Immunohistochemistry-Paraffin, RNA immunoprecipitation, Chemotaxis

Label

Unconjugated

Antibody Source

Polyclonal Rabbit IgG

Format

BSA Free
View all KMT2A/MLL Primary Antibodies »

Product Specifications

Immunogen

The immunogen recognized by this antibody maps to a region between residues 720 and 780 of human myeloid/lymphoid or mixed-lineage leukemia 1 using the number given in Swiss-Prot entry Q03164 (GeneID 4297).

Reactivity Notes

Mouse reactivity reported in scientific literature (PMID: 31786140).

Clonality

Polyclonal

Host

Rabbit

Isotype

IgG

Scientific Data Images for KMT2A/MLL Antibody - BSA Free

Western Blot: KMT2A/MLL Antibody [NB600-248]

Western Blot: KMT2A/MLL Antibody [NB600-248]

Western Blot: KMT2A/MLL Antibody [NB600-248] - Samples: Nuclear extract (50 and 15 ug) from HeLa, 293T, and Jurkat cells. Antibody: Affinity purified rabbit anti-MLL1 antibody used for WB at 0.1 ug/ml. Detection: Chemiluminescence with an exposure time of 3 minutes.
Immunoprecipitation: KMT2A/MLL Antibody [NB600-248]

Immunoprecipitation: KMT2A/MLL Antibody [NB600-248]

Immunoprecipitation: KMT2A/MLL Antibody [NB600-248] - Samples: Nuclear Extract (0.5 or 1.0 mg per IP reaction; 20% of IP loaded) from HeLa cells. Antibodies: Affinity purified rabbit anti-MLL1 antibody NB600-248 used for IP at 6 ug per reaction. MLL1 was also immunoprecipitated by rabbit anti-MLL1 antibody NB600-249. For blotting immunoprecipitated MLL1, NB600-248 was used at 1 ug/ml. Detection: Chemiluminescence with an exposure time of 10 seconds.

Applications for KMT2A/MLL Antibody - BSA Free

Application
Recommended Usage

Immunohistochemistry-Paraffin

1:100-1:250

Immunoprecipitation

2 - 10 ug/mg of lysate

Western Blot

1:500-1:5000
Application Notes
Use in IHC-P reported in scientific literature (PMID: 27396339).

Reviewed Applications

Read 1 review rated 4 using NB600-248 in the following applications:

Formulation, Preparation, and Storage

Purification

Immunogen affinity purified

Formulation

Tris-Citrate/Phosphate (pH 7.0 - 8.0)

Format

BSA Free

Preservative

0.09% Sodium Azide

Concentration

1.0 mg/ml

Shipping

The product is shipped with polar packs. Upon receipt, store it immediately at the temperature recommended below.

Stability & Storage

Store at 4C. Do not freeze.

Background: KMT2A/MLL

A partial tandem duplication within the MLL-gene (MLL-PTD) can be found in 8% of all patients with karyotypically normal acute myeloid leukemia (AML). MLL possibly acts as a transcriptional regulatory factor.

Alternate Names

ALL1, ALL-1MLL/GAS7, CXXC7TET1-MLL, CXXC-type zinc finger protein 7, EC 2.1.1.43, HRXFLJ11783, HTRX, HTRX1MLL-AF4 der(11) fusion protein, KMT2ACDK6/MLL fusion protein, Lysine N-methyltransferase 2A, MLL/GAS7 fusion protein, MLL/GMPS fusion protein, MLL1, MLL1A, myeloid/lymphoid or mixed-lineage leukemia (trithorax (Drosophila) homolog), myeloid/lymphoid or mixed-lineage leukemia (trithorax homolog, Drosophila), Trithorax-like protein, TRX1histone-lysine N-methyltransferase MLL, Zinc finger protein HRX

Entrez Gene IDs

4297 (Human); 214162 (Mouse)

Gene Symbol

KMT2A

UniProt

Q03164 (Human)

Additional KMT2A/MLL Products

Product Documents for KMT2A/MLL Antibody - BSA Free

Certificate of Analysis

To download a Certificate of Analysis, please enter a lot or batch number in the search box below.

Product Specific Notices for KMT2A/MLL Antibody - BSA Free

This product is for research use only and is not approved for use in humans or in clinical diagnosis. Primary Antibodies are guaranteed for 1 year from date of receipt.

Citations for KMT2A/MLL Antibody - BSA Free

Customer Reviews for KMT2A/MLL Antibody - BSA Free (1)

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  • Name: Anonymous
    Application: Western Blot
    Sample Tested: Cell lysate from Jurkat cells grown in RPMI medium
    Species: Human
    Verified Customer | Posted 08/27/2014
    MLL immunoblot in Jurkat cells
    KMT2A/MLL Antibody - BSA Free NB600-248

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