Ku70/XRCC6 Antibody (7A9E7) - BSA Free

Novus Biologicals | Catalog # NBP2-52590

Novus Biologicals
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Key Product Details

Species Reactivity

Human

Applications

Western Blot, ELISA, Flow Cytometry, Immunocytochemistry/ Immunofluorescence, CyTOF-ready

Label

Unconjugated

Antibody Source

Monoclonal Mouse IgG1 Clone # 7A9E7

Format

BSA Free
View all Ku70/XRCC6 Primary Antibodies »

Product Specifications

Immunogen

Purified recombinant fragment of human Ku70/XRCC6 (AA: 6-214) expressed in E. Coli.

Clonality

Monoclonal

Host

Mouse

Isotype

IgG1

Theoretical MW

69.8 kDa.
Disclaimer note: The observed molecular weight of the protein may vary from the listed predicted molecular weight due to post translational modifications, post translation cleavages, relative charges, and other experimental factors.

Scientific Data Images for Ku70/XRCC6 Antibody (7A9E7) - BSA Free

Western Blot: Ku70/XRCC6 Antibody (7A9E7)BSA Free [NBP2-52590]

Western Blot: Ku70/XRCC6 Antibody (7A9E7)BSA Free [NBP2-52590]

Western Blot: Ku70/XRCC6 Antibody (7A9E7) [NBP2-52590] - Analysis using XRCC6 mAb against human XRCC6 (AA: 6-214) recombinant protein. (Expected MW is 49.7 kDa)
Immunocytochemistry/ Immunofluorescence: Ku70/XRCC6 Antibody (7A9E7) - BSA Free [NBP2-52590]

Immunocytochemistry/ Immunofluorescence: Ku70/XRCC6 Antibody (7A9E7) - BSA Free [NBP2-52590]

Immunocytochemistry/Immunofluorescence: Ku70/XRCC6 Antibody (7A9E7) [NBP2-52590] - Analysis of MCF-7 cells using XRCC6 mouse mAb (green). Blue: DRAQ5 fluorescent DNA dye. Red: Actin filaments have been labeled with Alexa Fluor-555 phalloidin.
Flow Cytometry: Ku70/XRCC6 Antibody (7A9E7) - BSA Free [NBP2-52590]

Flow Cytometry: Ku70/XRCC6 Antibody (7A9E7) - BSA Free [NBP2-52590]

Flow Cytometry: Ku70/XRCC6 Antibody (7A9E7) [NBP2-52590] - Analysis of A431 cells using XRCC6 mouse mAb (green) and negative control (red).
Western Blot: Ku70/XRCC6 Antibody (7A9E7)BSA Free [NBP2-52590]

Western Blot: Ku70/XRCC6 Antibody (7A9E7)BSA Free [NBP2-52590]

Western Blot: Ku70/XRCC6 Antibody (7A9E7) [NBP2-52590] - Analysis using XRCC6 mAb against HEK293 (1) and XRCC6 (AA: 6-214)-hIgGFc transfected HEK293 (2) cell lysate.
ELISA: Ku70/XRCC6 Antibody (7A9E7) - BSA Free [NBP2-52590]

ELISA: Ku70/XRCC6 Antibody (7A9E7) - BSA Free [NBP2-52590]

ELISA: Ku70/XRCC6 Antibody (7A9E7) [NBP2-52590] - Black line: Control Antigen (100 ng); Purple line: Antigen(10ng); Blue line: Antigen (50 ng); Red line: Antigen (100 ng).
Ku70/XRCC6 Antibody (7A9E7) [NBP2-52590] - Analysis using XRCC6 mouse mAb against PC-2 (1), A549 (2), A431 (3), HepG2 (4), K562 (5) cell lysate.

Applications for Ku70/XRCC6 Antibody (7A9E7) - BSA Free

Application
Recommended Usage

ELISA

1:10000

Flow Cytometry

1:200-1:400

Immunocytochemistry/ Immunofluorescence

1:200-1:1000

Western Blot

1:500-1:2000
Application Notes
This antibody is Cytof ready.

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Advanced Features

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Flow Cytometry

Formulation, Preparation, and Storage

Purification

Protein G purified

Formulation

PBS

Format

BSA Free

Preservative

0.05% Sodium Azide

Concentration

1 mg/ml

Shipping

The product is shipped with polar packs. Upon receipt, store it immediately at the temperature recommended below.

Stability & Storage

Store at 4C short term. Aliquot and store at -20C long term. Avoid freeze-thaw cycles.

Background: Ku70/XRCC6

The Ku autoantigen is a heterodimer of 70kDa (p70) and ~80kDa (p80) proteins. The p70/p80 dimer is important for function of a 460kDa DNAdependent protein kinase that phosphorylates certain transcription factors, including Sp1, Oct-1, p53, and SV40 large T antigen in vitro. Ku protein plays a role in cell signaling, proliferation, DNA repair, replication, transcriptional activation, and apoptosis.

Long Name

Lupus Ku Autoantigen Protein 70

Alternate Names

CTC75, CTCBF, G22P1, ML8, TLAA, XRCC6

Gene Symbol

XRCC6

Additional Ku70/XRCC6 Products

Product Documents for Ku70/XRCC6 Antibody (7A9E7) - BSA Free

Certificate of Analysis

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Product Specific Notices for Ku70/XRCC6 Antibody (7A9E7) - BSA Free

This product is for research use only and is not approved for use in humans or in clinical diagnosis. Primary Antibodies are guaranteed for 1 year from date of receipt.

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Protocols

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FAQs

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