Ku70/XRCC6 Antibody - BSA Free

Novus Biologicals | Catalog # NBP2-19311

Novus Biologicals
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Key Product Details

Species Reactivity

Human, Mouse

Applications

Immunohistochemistry, Immunohistochemistry-Paraffin, Western Blot, Immunocytochemistry/ Immunofluorescence, Immunoprecipitation, Proximity Ligation Assay

Label

Unconjugated

Antibody Source

Polyclonal Rabbit IgG

Format

BSA Free
View all Ku70/XRCC6 Primary Antibodies »

Product Specifications

Immunogen

Recombinant protein encompassing a sequence within the C-terminus region of human Ku70/XRCC6. The exact sequence is proprietary.

Localization

Nucleus

Clonality

Polyclonal

Host

Rabbit

Isotype

IgG

Theoretical MW

70 kDa.
Disclaimer note: The observed molecular weight of the protein may vary from the listed predicted molecular weight due to post translational modifications, post translation cleavages, relative charges, and other experimental factors.

Scientific Data Images for Ku70/XRCC6 Antibody - BSA Free

Western Blot: Ku70/XRCC6 Antibody [NBP2-19311]

Western Blot: Ku70/XRCC6 Antibody [NBP2-19311]

Western Blot: Ku70/XRCC6 Antibody [NBP2-19311] - Various whole cell extracts (30 ug) were separated by 7.5% SDS-PAGE, and the membrane was blotted with Ku70 antibody diluted by 1:2000. The HRP-conjugated anti-rabbit IgG antibody (NBP2-19301) was used to detect the primary antibody.
Immunohistochemistry-Paraffin: Ku70/XRCC6 Antibody [NBP2-19311]

Immunohistochemistry-Paraffin: Ku70/XRCC6 Antibody [NBP2-19311]

Immunohistochemistry-Paraffin: Ku70/XRCC6 Antibody [NBP2-19311] - Human cervical cancer. Ku70 antibody diluted at 1:500. Antigen Retrieval: Citrate buffer, pH 6.0, 15 min.
Immunohistochemistry-Paraffin: Ku70/XRCC6 Antibody [NBP2-19311]

Immunohistochemistry-Paraffin: Ku70/XRCC6 Antibody [NBP2-19311]

Immunohistochemistry-Paraffin: Ku70 Antibody [NBP2-19311] - Immunohistochemical analysis of paraffin-embedded D54 xenograft, using antibody at 1:500 dilution.
Immunoprecipitation: Ku70/XRCC6 Antibody [NBP2-19311]

Immunoprecipitation: Ku70/XRCC6 Antibody [NBP2-19311]

Immunoprecipitation: Ku70 Antibody [NBP2-19311] - Sample: 1000 ug HeLa whole cell lysate/extract A. 40 ug HeLa whole cell lysate/extract B. Control with 2. 5 ug of preimmune rabbit IgG C. Immunoprecipitation of Ku70 protein by 2. 5 ug of Ku70 antibody 7. 5% SDS-PAGE The immunoprecipitated Ku70 protein was detected by Ku70 antibody diluted at 1:1000. anti-rabbit IgG was used as a secondary reagent.
Ku70/XRCC6 Antibody - BSA Free

Immunocytochemistry/ Immunofluorescence: Ku70/XRCC6 Antibody [NBP2-19311] -

Ku70 antibody detects Ku70 protein at nucleus by immunofluorescent analysis.
Samples: HeLa cells were fixed in 4% paraformaldehyde at RT for 15 min.
Green: Ku70 protein stained by Ku70 antibody (NBP2-19311) diluted at 1:200.
Red: phalloidin, a cytoskeleton marker, diluted at 1:200.
Scale bar = 10 um.

Applications for Ku70/XRCC6 Antibody - BSA Free

Application
Recommended Usage

Immunocytochemistry/ Immunofluorescence

1:100-1:1000

Immunohistochemistry

1:100-1:1000

Immunohistochemistry-Paraffin

1:100-1:1000

Immunoprecipitation

1:500-1:1000

Proximity Ligation Assay

Assay dependent

Western Blot

1:500-1:3000

Formulation, Preparation, and Storage

Purification

Antigen Affinity-purified

Formulation

PBS, 20% Glycerol

Format

BSA Free

Preservative

0.025% Proclin 300

Concentration

Concentrations vary lot to lot. See vial label for concentration. If unlisted please contact technical services.

Shipping

The product is shipped with polar packs. Upon receipt, store it immediately at the temperature recommended below.

Stability & Storage

Aliquot and store at -20C or -80C. Avoid freeze-thaw cycles.

Background: Ku70/XRCC6

The p70/p80 autoantigen is a nuclear complex consisting of two subunits with molecular masses of approximately 70 and 80 kDa. The complex functions as a single-stranded DNA-dependent ATP-dependent helicase. The complex may be involved in the repair of nonhomologous DNA ends such as that required for double-strand break repair, transposition, and V(D)J recombination. High levels of autoantibodies to p70 and p80 have been found in some patients with systemic lupus erythematosus. [provided by RefSeq]

Long Name

Lupus Ku Autoantigen Protein 70

Alternate Names

CTC75, CTCBF, G22P1, ML8, TLAA, XRCC6

Gene Symbol

XRCC6

UniProt

P12956

Additional Ku70/XRCC6 Products

Product Documents for Ku70/XRCC6 Antibody - BSA Free

Certificate of Analysis

To download a Certificate of Analysis, please enter a lot or batch number in the search box below.

Product Specific Notices for Ku70/XRCC6 Antibody - BSA Free

This product is for research use only and is not approved for use in humans or in clinical diagnosis. Primary Antibodies are guaranteed for 1 year from date of receipt.

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Protocols

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FAQs

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