Kv4.2 Antibody - BSA Free

Novus Biologicals | Catalog # NBP3-03748

Novus Biologicals
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Key Product Details

Species Reactivity

Mouse

Applications

Immunohistochemistry, Immunohistochemistry-Paraffin, Western Blot

Label

Unconjugated

Antibody Source

Polyclonal Rabbit IgG

Format

BSA Free
View all Kv4.2 Primary Antibodies »

Product Specifications

Immunogen

Recombinant fusion protein containing a sequence corresponding to amino acids 501-630 of human Kv4.2 (NP_036413.1). ESCMEVATVNRPSSHSPSLSSQQGVTSTCCSRRHKKTFRIPNANVSGSHQGSIQELSTIQIRCVERTPLSNSRSSLNAKMEECVKLNCEQPYVTTAIISIPTPPVTTPEGDDRPESPEYSGGNIVRVSAL

Clonality

Polyclonal

Host

Rabbit

Isotype

IgG

Theoretical MW

70 kDa.
Disclaimer note: The observed molecular weight of the protein may vary from the listed predicted molecular weight due to post translational modifications, post translation cleavages, relative charges, and other experimental factors.

Scientific Data Images for Kv4.2 Antibody - BSA Free

Kv4.2 Antibody - BSA Free

Western Blot: Kv4.2 Antibody - BSA Free [NBP3-03748] -

Western Blot: Kv4.2 Antibody - BSA Free [NBP3-03748] - Western blot analysis of Mouse heart, using Kv4.2 antibody (A6203) at 1:3000 dilution.
Secondary antibody: HRP Goat Anti-Rabbit IgG (H+L) (AS014) at 1:10000 dilution.
Lysates/proteins: 25μg per lane.
Blocking buffer: 3% nonfat dry milk in TBST.
Detection: ECL Basic Kit (RM00020).
Exposure time: 60s.

Applications for Kv4.2 Antibody - BSA Free

Application
Recommended Usage

Immunohistochemistry

1:50 - 1:200

Western Blot

1:1000 - 1:5000
Application Notes
Kv4.2 antibody validated for IHC-P from a verified customer review.

Reviewed Applications

Read 1 review rated 5 using NBP3-03748 in the following applications:

Formulation, Preparation, and Storage

Purification

Affinity purified

Formulation

PBS (pH 7.3), 50% glycerol

Format

BSA Free

Preservative

0.02% Sodium Azide

Concentration

Please see the vial label for concentration. If unlisted please contact technical services.

Shipping

The product is shipped with polar packs. Upon receipt, store it immediately at the temperature recommended below.

Stability & Storage

Store at -20C. Avoid freeze-thaw cycles.

Background: Kv4.2

Kv4.2, a voltage-gated K+ (Kv) channel subunit, has been suggested to be the key component of the subthreshold A-type K+ currents (I(SA)s) recorded from the specific subcellular compartments of certain CNS neurons (1). A-type channels, encoded by the pore-forming alpha-subunits of the Kv4.x family, are particularly important in regulating membrane excitability in the CNS and the heart. Kv4.2 is a Shal-type potassium channel that passes an A-type current and is localized to dendrites and cell bodies in the hippocampus (2). Deletion of the Kv4.2 gene and a loss of Kv4.2 protein resulted in a specific and near-complete elimination of A-type K+ currents from the apical dendrites of CA1 pyramidal neurons. The absence of dendritic Kv4.2-encoded A-type K+ currents led to an increase of bAP amplitude and an increase of concurrent Ca2+ influx (3).

Alternate Names

potassium voltage-gated channel, Shal-related subfamily, member 2, RK5, voltage-gated potassium channel Kv4.2, voltage-sensitive potassium channel

Gene Symbol

KCND2

Additional Kv4.2 Products

Product Documents for Kv4.2 Antibody - BSA Free

Certificate of Analysis

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Product Specific Notices for Kv4.2 Antibody - BSA Free

This product is for research use only and is not approved for use in humans or in clinical diagnosis. Primary Antibodies are guaranteed for 1 year from date of receipt.

Customer Reviews for Kv4.2 Antibody - BSA Free (1)

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  • Kv4.2 Antibody
    Name: Ike Chinyere
    Application: Immunohistochemistry-Paraffin
    Sample Tested: cardiac tissue
    Species: Rat
    Verified Customer | Posted 12/16/2020
    Rat model of heart failure post-myocardial infarction, Kv4.2 IHC in infarct region with nuclear counterstain. 60X magnification
    1:250 dilution Nuclear counterstain
    Kv4.2 Antibody - BSA Free NBP3-03748

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