L-15 (Leibovitz), 25 mM HEPES, GlutaminePlus, no phenol red
L-15 (Leibovitz), 25 mM HEPES, GlutaminePlus, no phenol red Summary
What is Leibovitz's L-15 Medium?
Leibovitz’s L-15 Medium was formulated for use in a carbon dioxide (CO2) free environment. It contains no sodium bicarbonate, and is buffered by phosphates, free base amino acids, and galactose instead. L-15 (Leibovitz) supports the proliferation of many established cell lines and primary cells. L-15 is offered with HEPES buffer for a more effective buffering capacity.
Each lot of L-15 is prepared from powdered base medium, tissue culture-grade water, and is sterile filtered using a 0.2 micron filter. Representative samples of each lot of L-15 are tested to confirm the absence of bacterial or fungal contamination using methods adapted from the current U.S. Pharmacopeia. L-15 is manufactured in our ISO 9001:2015 certified facility.
or the specific media formulation, please refer to the Media Formulation section of the datasheet.
For research use only. Not for diagnostic use.
What is GlutaminePlus, and how does it compare to L-Glutamine?
GlutaminePlus, a derivative of L-glutamine obtained by a chemical reaction of L-alanine with L-glutamine, is available in liquid form or as an ingredient in ready-to-use Atlanta Biologicals brand cell culture media (media with stable L-glutamine).
GlutaminePlus is metabolized within the cells to yield L-glutamine plus the second amino acid. This results in more consistent delivery of L-glutamine to cells in culture and avoids toxic buildup of ammonia in cell cultures. This feature can be especially important for ammonia sensitive cell lines.
L-glutamine is an essential amino acid and plays a major role for the growth and function of cells in culture. Although L-glutamine is stable in crystalline form, it has the tendency to degrade non-enzymatically and irreversibly in solution within a short time period. The rate of this L-glutamine breakdown is dependent on pH, temperature and the presence of various anions.
One of the by-products, ammonia, may act as a toxin or growth inhibitor for the cultured cells. Because of its chemical instability and significance for cell growth, it is important that the delivery of L-glutamine be optimized to each specific cell culture application. A recommended way of achieving reliable delivery of L-glutamine to the cells in culture is the use of stable derivatives of L-glutamine in cell culture media.
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