L-15 (Leibovitz), 25 mM HEPES, GlutaminePlus, no phenol red

Atlanta Biologicals media and balanced salt solutions are now sold under the R&D Systems brand
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L-15 (Leibovitz), 25 mM HEPES, GlutaminePlus, no phenol red
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L-15 (Leibovitz), 25 mM HEPES, GlutaminePlus, no phenol red Summary

Leibovitz’s L-15 Medium with 25 mM HEPES buffer and GlutaminePlus. No phenol red.

What is Leibovitz's L-15 Medium?

Leibovitz’s L-15 Medium was formulated for use in a carbon dioxide (CO2) free environment. It contains no sodium bicarbonate, and is buffered by phosphates, free base amino acids, and galactose instead. L-15 (Leibovitz) supports the proliferation of many established cell lines and primary cells. L-15 is offered with HEPES buffer for a more effective buffering capacity.

Each lot of L-15 is prepared from powdered base medium, tissue culture-grade water, and is sterile filtered using a 0.2 micron filter. Representative samples of each lot of L-15 are tested to confirm the absence of bacterial or fungal contamination using methods adapted from the current U.S. Pharmacopeia. L-15 is manufactured in our ISO 9001:2015 certified facility.

or the specific media formulation, please refer to the Media Formulation section of the datasheet.


0.2 μm filtered
Sterility Testing
Shipping Conditions
2 - 8 °C, protected from light
Cell Culture
Country of Origin


For research use only. Not for diagnostic use.

Product Datasheets


  1. What is GlutaminePlus, and how does it compare to L-Glutamine?

    • GlutaminePlus, a derivative of L-glutamine obtained by a chemical reaction of L-alanine with L-glutamine, is available in liquid form or as an ingredient in ready-to-use Atlanta Biologicals brand cell culture media (media with stable L-glutamine).

      GlutaminePlus is metabolized within the cells to yield L-glutamine plus the second amino acid. This results in more consistent delivery of L-glutamine to cells in culture and avoids toxic buildup of ammonia in cell cultures. This feature can be especially important for ammonia sensitive cell lines.

      L-glutamine is an essential amino acid and plays a major role for the growth and function of cells in culture. Although L-glutamine is stable in crystalline form, it has the tendency to degrade non-enzymatically and irreversibly in solution within a short time period. The rate of this L-glutamine breakdown is dependent on pH, temperature and the presence of various anions.

      One of the by-products, ammonia, may act as a toxin or growth inhibitor for the cultured cells. Because of its chemical instability and significance for cell growth, it is important that the delivery of L-glutamine be optimized to each specific cell culture application. A recommended way of achieving reliable delivery of L-glutamine to the cells in culture is the use of stable derivatives of L-glutamine in cell culture media.

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