Key Product Details

Species Reactivity

Human

Applications

Western Blot, Immunocytochemistry, Flow Cytometry (Negative)

Label

Unconjugated

Antibody Source

Monoclonal Rabbit IgG Clone # 2066A

Format

Azide and BSA Free
View all Lactate Dehydrogenase A/LDHA Primary Antibodies »

Product Specifications

Immunogen

rhLDH-A aa2-92

Clonality

Monoclonal

Host

Rabbit

Isotype

IgG

Description

>90% by SDS PAGE

Scientific Data Images for Lactate Dehydrogenase A/LDHA Antibody (2066A) - Azide and BSA Free

Western Blot: Lactate Dehydrogenase A/LDHA Antibody (2066A)Azide and BSA Free [MAB9216]

Western Blot: Lactate Dehydrogenase A/LDHA Antibody (2066A)Azide and BSA Free [MAB9216]

Western Blot: Lactate Dehydrogenase A/LDHA Antibody (2066A) [MAB9216] - Analysis of HepG2, Daudi and MOLT-4 cells lysates with 0.5 ug/ml of hLDHA/LDHB Monoclonal Antibody followed by HRP-conjugated Anti-Rabbit IgG Antibody. A specific band was detected for hLDHA/LDHB at approximately 34-36 kDa (as indicated). This experiment was conducted under reducing conditions and using Immunoblot Buffer Group 1.
Immunocytochemistry: Lactate Dehydrogenase A/LDHA Antibody (2066A) - Azide and BSA Free [MAB9216]

Immunocytochemistry: Lactate Dehydrogenase A/LDHA Antibody (2066A) - Azide and BSA Free [MAB9216]

Immunocytochemistry: Lactate Dehydrogenase A/LDHA Antibody (2066A) [MAB9216] - Lactate Dehydrogenase A/LDHA was detected in immersion fixed PC3 cell using Lactate Dehydrogenase A/LDHA Monoclonal Antibody at 1 ug/ml dilution. Cells were stained using Donkey anti-Rabbit IgG Secondary Antibody [NL557] (red) and counterstained with DAPI (blue). Specific staining was localized to cytoplasmic.

Applications for Lactate Dehydrogenase A/LDHA Antibody (2066A) - Azide and BSA Free

Application
Recommended Usage

Flow Cytometry (Negative)

Optimal dilutions of this antibody should be experimentally determined.

Immunocytochemistry

Optimal dilutions of this antibody should be experimentally determined.

Western Blot

Optimal dilutions of this antibody should be experimentally determined.

Formulation, Preparation, and Storage

Purification

Protein A or G purified

Formulation

PBS

Format

Azide and BSA Free

Preservative

No Preservative

Concentration

1.0 mg/ml

Shipping

The product is shipped with polar packs. Upon receipt, store it immediately at the temperature recommended below.

Stability & Storage

Store at -20C in the dark. Avoid freeze-thaw cycles.

Background: Lactate Dehydrogenase A/LDHA

Lactate dehydrogenase (LDH) is an ubiquitous enzyme commonly found in wide variety of organisms, including plants and microbes. LDH is involved in the interconversion of the pyruvate and NADH to lactate and NAD+. It is also called Hydroxybutyrate Dehydrogenase (HBD), due to the fact that it can catalyze the oxidation of hydroxybutyrate (1). In mammals, three types of LDH subunits (35 kDa) are encoded by the genes Ldh-A, Ldh-B, and Ldh-C, forming various terameric isoenzymes (140 kDa). Lactate dehydrogenase A (LDH-A, muscle subunit, LDH-M) is involved in the final step of anaerobic glycoysis and catalyzes the conversion of L-lactate and NAD to pryruvate and NADH. While it is predominantly expressed in muscle tissue, it is hormonally regulated in rodents and overexpressed during mammary gland tumorigenesis (2). A mutation that cause deficiency in LDH-A has been implicated in exertional myoglobinuria (3).

Alternate Names

LDH1, LDHA, LDHM

Gene Symbol

LDHA

Additional Lactate Dehydrogenase A/LDHA Products

Product Documents for Lactate Dehydrogenase A/LDHA Antibody (2066A) - Azide and BSA Free

Certificate of Analysis

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Product Specific Notices for Lactate Dehydrogenase A/LDHA Antibody (2066A) - Azide and BSA Free

This product is for research use only and is not approved for use in humans or in clinical diagnosis. Primary Antibodies are guaranteed for 1 year from date of receipt.

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Protocols

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FAQs

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