Lactate Dehydrogenase C Antibody - BSA Free

Novus Biologicals | Catalog # NBP1-92065

Novus Biologicals
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Key Product Details

Validated by

Orthogonal Validation

Species Reactivity

Human

Applications

Immunohistochemistry, Immunohistochemistry-Paraffin

Label

Unconjugated

Antibody Source

Polyclonal Rabbit IgG

Format

BSA Free
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Product Specifications

Immunogen

This antibody was developed against Recombinant Protein corresponding to amino acids: MSTVKEQLIEKLIEDDENSQCKITIVGTGAVG

Clonality

Polyclonal

Host

Rabbit

Isotype

IgG

Scientific Data Images for Lactate Dehydrogenase C Antibody - BSA Free

Immunohistochemistry-Paraffin: Lactate Dehydrogenase C Antibody [NBP1-92065]

Immunohistochemistry-Paraffin: Lactate Dehydrogenase C Antibody [NBP1-92065]

Immunohistochemistry-Paraffin: Lactate Dehydrogenase C Antibody [NBP1-92065] - Staining in human testis and prostate tissues using anti-LDHC antibody. Corresponding LDHC RNA-seq data are presented for the same tissues.
Immunohistochemistry-Paraffin: Lactate Dehydrogenase C Antibody [NBP1-92065]

Immunohistochemistry-Paraffin: Lactate Dehydrogenase C Antibody [NBP1-92065]

Immunohistochemistry-Paraffin: Lactate Dehydrogenase C Antibody [NBP1-92065] - Staining of human testis shows strong cytoplasmic and nuclear positivity in cells in seminiferus ducts.
Immunohistochemistry-Paraffin: Lactate Dehydrogenase C Antibody [NBP1-92065]

Immunohistochemistry-Paraffin: Lactate Dehydrogenase C Antibody [NBP1-92065]

Immunohistochemistry-Paraffin: Lactate Dehydrogenase C Antibody [NBP1-92065] - Staining of human prostate shows low expression as expected.
Lactate Dehydrogenase C Antibody - BSA Free Immunohistochemistry: Lactate Dehydrogenase C Antibody - BSA Free [NBP1-92065]

Immunohistochemistry: Lactate Dehydrogenase C Antibody - BSA Free [NBP1-92065]

Staining of human testis shows high expression.

Applications for Lactate Dehydrogenase C Antibody - BSA Free

Application
Recommended Usage

Immunohistochemistry

1:2500 - 1:5000

Immunohistochemistry-Paraffin

1:2500 - 1:5000
Application Notes
For IHC-Paraffin, HIER pH 6 retrieval is recommended. Immunocytochemistry/Immunofluorescence Fixation Permeabilization: Use PFA/Triton X-100.

Reviewed Applications

Read 1 review rated 3 using NBP1-92065 in the following applications:

Formulation, Preparation, and Storage

Purification

Affinity purified

Formulation

PBS (pH 7.2) and 40% Glycerol

Format

BSA Free

Preservative

0.02% Sodium Azide

Concentration

Concentrations vary lot to lot. See vial label for concentration. If unlisted please contact technical services.

Shipping

The product is shipped with polar packs. Upon receipt, store it immediately at the temperature recommended below.

Stability & Storage

Store at 4C short term. Aliquot and store at -20C long term. Avoid freeze-thaw cycles.

Background: Lactate Dehydrogenase C

Lactate dehydrogenase (LDH) is an ubiquitous enzyme commonly found in wide variety of organisms, including plants and microbes. LDH is involved in the interconversion of the pyruvate and NADH to lactate and NAD+. It is also called Hydroxybutyrate Dehydrogenase (HBD), because it can catalyze the oxidation of hydroxybutyrate (1). In mammals, three types of LDH subunits (35 kDa) are encoded by the genes Ldh-A, Ldh-B, and Ldh-C. All LDH subunits can combine to form various terameric isoenzymes (140 kDa) (2). LDHA and LDHB are expressed in most somatic tissues, while expression of LDHC is confined to the germinal epithelium of the testes (3)

Alternate Names

Cancer/testis antigen 32, CT32EC 1.1.1.27, EC 1.1.1, lactate dehydrogenase C, lactate dehydrogenase C4, LDH testis subunit, LDH3MGC111073, LDH-C, LDHX, LDH-X, L-lactate dehydrogenase C chain

Gene Symbol

LDHC

Additional Lactate Dehydrogenase C Products

Product Documents for Lactate Dehydrogenase C Antibody - BSA Free

Certificate of Analysis

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Product Specific Notices for Lactate Dehydrogenase C Antibody - BSA Free

This product is for research use only and is not approved for use in humans or in clinical diagnosis. Primary Antibodies are guaranteed for 1 year from date of receipt.

Customer Reviews for Lactate Dehydrogenase C Antibody - BSA Free (1)

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  • Name: Corey.
    Application: Immunocytochemistry
    Sample Tested: A549 human alveolar adenocarcinoma cell line
    Species: Human
    Verified Customer | Posted 04/17/2013
    Lactate Dehydrogenase C Antibody - BSA Free NBP1-92065

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Protocols

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FAQs for Lactate Dehydrogenase C Antibody - BSA Free

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  • Q: What isotype is this antibody?

    A: NBP1-92065 is an IgG isotype antibody.

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