Lamin B1 Antibody (4B10) - Azide and BSA Free

Novus Biologicals | Catalog # H00004001-M01

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Key Product Details

Species Reactivity

Validated:

Human, Mouse, Rat

Cited:

Human

Applications

Validated:

Western Blot, ELISA, Immunocytochemistry/ Immunofluorescence, Immunoprecipitation

Cited:

Western Blot

Label

Unconjugated

Antibody Source

Monoclonal Mouse IgG2a Kappa Clone # 4B10

Format

Azide and BSA Free
View all Lamin B1 Primary Antibodies »

Product Specifications

Immunogen

LMNB1 (NP_005564.1, 107 a.a. ~ 186 a.a) partial recombinant protein with GST tag. MW of the GST tag alone is 26 KDa. LGKCKAEHDQLLLNYAKKESDLNGAQIKLREYEAALNSKDAALATALGDKKSLEGDLEDLKDQIAQLEASLAAAKKQLAD

Reactivity Notes

Please note that this antibody is reactive to Mouse and derived from the same host, Mouse. Additional Mouse on Mouse blocking steps may be required for IHC and ICC experiments. Please contact Technical Support for more information.

Marker

Nuclear Envelope Marker

Specificity

LMNB1 - lamin B1 (4B10)

Clonality

Monoclonal

Host

Mouse

Isotype

IgG2a Kappa

Description

Quality control test: Antibody Reactive Against Recombinant Protein.

Scientific Data Images for Lamin B1 Antibody (4B10) - Azide and BSA Free

Western Blot: Lamin B1 Antibody (4B10) [H00004001-M01]

Western Blot: Lamin B1 Antibody (4B10) [H00004001-M01]

Western Blot: Lamin B1 Antibody (4B10) [H00004001-M01] - Analysis of LMNB1 expression in transfected 293T cell line by LMNB1 monoclonal antibody (M01), clone 4B10.Lane 1: LMNB1 transfected lysate(66.4 KDa).Lane 2: Non-transfected lysate.
Immunocytochemistry/ Immunofluorescence: Lamin B1 Antibody (4B10) [H00004001-M01]

Immunocytochemistry/ Immunofluorescence: Lamin B1 Antibody (4B10) [H00004001-M01]

Immunocytochemistry/Immunofluorescence: Lamin B1 Antibody (4B10) [H00004001-M01] - Analysis of monoclonal antibody to LMNB1 on HeLa cell. Antibody concentration 10 ug/ml.
Western Blot: Lamin B1 Antibody (4B10) [H00004001-M01]

Western Blot: Lamin B1 Antibody (4B10) [H00004001-M01]

Western Blot: Lamin B1 Antibody (4B10) [H00004001-M01] - LMNB1 monoclonal antibody (M01), clone 4B10. Analysis of LMNB1 expression in Raw 264.7.
Western Blot: Lamin B1 Antibody (4B10) [H00004001-M01]

Western Blot: Lamin B1 Antibody (4B10) [H00004001-M01]

Western Blot: Lamin B1 Antibody (4B10) [H00004001-M01] - LMNB1 monoclonal antibody (M01), clone 4B10. Analysis of LMNB1 expression in Jurkat.
Western Blot: Lamin B1 Antibody (4B10) [H00004001-M01]

Western Blot: Lamin B1 Antibody (4B10) [H00004001-M01]

Western Blot: Lamin B1 Antibody (4B10) [H00004001-M01] - LMNB1 monoclonal antibody (M01), clone 4B10. Analysis of LMNB1 expression in NIH/3T3.
Immunoprecipitation: Lamin B1 Antibody (4B10) [H00004001-M01]

Immunoprecipitation: Lamin B1 Antibody (4B10) [H00004001-M01]

Immunoprecipitation: Lamin B1 Antibody (4B10) [H00004001-M01] - Analysis of LMNB1 transfected lysate using anti-LMNB1 monoclonal antibody and Protein A Magnetic Bead, and immunoblotted with LMNB1 MaxPab rabbit polyclonal antibody.
ELISA: Lamin B1 Antibody (4B10) [H00004001-M01]

ELISA: Lamin B1 Antibody (4B10) [H00004001-M01]

ELISA: Lamin B1 Antibody (4B10) [H00004001-M01] - Detection limit for recombinant GST tagged LMNB1 is 3 ng/ml as a capture antibody.

Applications for Lamin B1 Antibody (4B10) - Azide and BSA Free

Application
Recommended Usage

Immunocytochemistry/ Immunofluorescence

1:10-1:500

Immunoprecipitation

1:10-1:500

Western Blot

1:500
Application Notes
Antibody reactivity against Recombinant Protein with GST tag on ELISA and WB. GST tag alone is used as a negative control.

Formulation, Preparation, and Storage

Purification

IgG purified

Formulation

In 1x PBS, pH 7.4

Format

Azide and BSA Free

Preservative

No Preservative

Concentration

Concentrations vary lot to lot. See vial label for concentration. If unlisted please contact technical services.

Shipping

The product is shipped with polar packs. Upon receipt, store it immediately at the temperature recommended below.

Stability & Storage

Aliquot and store at -20C or -80C. Avoid freeze-thaw cycles.

Background: Lamin B1

The nuclear lamina consists of a two-dimensional matrix of proteins located next to the inner nuclear membrane. The lamin family of proteins make up the matrix and are highly conserved in evolution. During mitosis, the lamina matrix is reversibly disassembled as the lamin proteins are phosphorylated. Lamin proteins are thought to be involved in nuclear stability, chromatin structure and gene expression. Vertebrate lamins consist of two types, A and B. This gene encodes one of the two B type proteins, B1. [provided by RefSeq]

Alternate Names

ADLD, LMN2, LMNB1

Entrez Gene IDs

4001 (Human)

Gene Symbol

LMNB1

UniProt

NP_005564 (Human)

Additional Lamin B1 Products

Product Documents for Lamin B1 Antibody (4B10) - Azide and BSA Free

Certificate of Analysis

To download a Certificate of Analysis, please enter a lot or batch number in the search box below.

Download SDS

Product Specific Notices for Lamin B1 Antibody (4B10) - Azide and BSA Free

This product is produced by and distributed for Abnova, a company based in Taiwan.

This product is for research use only and is not approved for use in humans or in clinical diagnosis. Primary Antibodies are guaranteed for 1 year from date of receipt.

Related Research Areas

Cancer Biomarkers
Epigenetics

Citations for Lamin B1 Antibody (4B10) - Azide and BSA Free

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Protocols

Find general support by application which include: protocols, troubleshooting, illustrated assays, videos and webinars.

FAQs for Lamin B1 Antibody (4B10) - Azide and BSA Free

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  • Q: I'm looking for human lamin antibodies that might cross react with Drosophila lamins. I've found a few that I'm interested in, one of which comes in a smaller sample size. The other two do not have a smaller size ordering option. I was wondering if it would be possible to get these other antibodies in a smaller size as well, so we can test reactivity with Drosophila lamins before ordering the full-sized product?

    A:

    We have a wide range of antibodies to human Lamin. Unfortunately none of these has yet been tested against Drosophila samples, however you may be interested in our Innovator's Reward Program. This allows you to try our primary antibodies in an untested species or application, without the financial risk of failure. To participate you simply complete an online review with an image, detailing the positive or negative results of your study, and in return you receive a discount voucher for 100% of the purchase price of the reviewed product. We are unable to offer free samples of our antibodies but, as you rightly point out, some are available as smaller, sample size aliquots. If a sample size is not listed for a product I am afraid we are unable to offer a smaller aliquot than those already listed.

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