Lamin B1 Antibody (CL3929) - BSA Free

Novus Biologicals | Catalog # NBP2-59783

Novus Biologicals
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Key Product Details

Species Reactivity

Validated:

Human

Cited:

Human

Applications

Validated:

Immunohistochemistry, Immunohistochemistry-Paraffin, Western Blot, Immunocytochemistry/ Immunofluorescence

Cited:

IF/IHC

Label

Unconjugated

Antibody Source

Monoclonal Mouse IgG1 Clone # CL3929

Format

BSA Free
View all Lamin B1 Primary Antibodies »

Product Specifications

Immunogen

This antibody was developed using a synthetic peptide derived from P20700, with the exact immunogen sequence remaining proprietary.

Clonality

Monoclonal

Host

Mouse

Isotype

IgG1

Scientific Data Images for Lamin B1 Antibody (CL3929) - BSA Free

Western Blot: Lamin B1 Antibody (CL3929) [NBP2-59783]

Western Blot: Lamin B1 Antibody (CL3929) [NBP2-59783]

Western Blot: Lamin B1 Antibody (CL3929) [NBP2-59783] - Analysis in human cell line MCF-7.
Immunocytochemistry/ Immunofluorescence: Lamin B1 Antibody (CL3929) [NBP2-59783]

Immunocytochemistry/ Immunofluorescence: Lamin B1 Antibody (CL3929) [NBP2-59783]

Immunocytochemistry/Immunofluorescence: Lamin B1 Antibody (CL3929) [NBP2-59783] - Staining of A-431 cells showing specific staining of nuclear membrane in green. Microtubule and nuclear probes are visualized in red and blue, respectively (where available). Antibody staining is shown in green.
Immunohistochemistry-Paraffin: Lamin B1 Antibody (CL3929) [NBP2-59783]

Immunohistochemistry-Paraffin: Lamin B1 Antibody (CL3929) [NBP2-59783]

Immunohistochemistry-Paraffin: Lamin B1 Antibody (CL3929) [NBP2-59783] - Staining of human colon shows nuclear membrane positivity in glandular and connective tissue cells.
Immunocytochemistry/ Immunofluorescence: Lamin B1 Antibody (CL3929) [NBP2-59783]

Immunocytochemistry/ Immunofluorescence: Lamin B1 Antibody (CL3929) [NBP2-59783]

Immunocytochemistry/Immunofluorescence: Lamin B1 Antibody (CL3929) [NBP2-59783] - Staining of MCF7 cells showing specific staining of nuclear membrane in green. Microtubule and nuclear probes are visualized in red and blue, respectively (where available). Antibody staining is shown in green.
Immunocytochemistry/ Immunofluorescence: Lamin B1 Antibody (CL3929) [NBP2-59783]

Immunocytochemistry/ Immunofluorescence: Lamin B1 Antibody (CL3929) [NBP2-59783]

Immunocytochemistry/Immunofluorescence: Lamin B1 Antibody (CL3929) [NBP2-59783] - Staining of U-251 cells showing specific staining of nuclear membrane in green. Microtubule and nuclear probes are visualized in red and blue, respectively (where available). Antibody staining is shown in green.
Immunocytochemistry/ Immunofluorescence: Lamin B1 Antibody (CL3929) [NBP2-59783]

Immunocytochemistry/ Immunofluorescence: Lamin B1 Antibody (CL3929) [NBP2-59783]

Immunocytochemistry/Immunofluorescence: Lamin B1 Antibody (CL3929) [NBP2-59783] - Staining of HeLa cells showing specific staining of nuclear membrane in green. Microtubule and nuclear probes are visualized in red and blue, respectively (where available). Antibody staining is shown in green.
Immunocytochemistry/ Immunofluorescence: Lamin B1 Antibody (CL3929) [NBP2-59783]

Immunocytochemistry/ Immunofluorescence: Lamin B1 Antibody (CL3929) [NBP2-59783]

Immunocytochemistry/Immunofluorescence: Lamin B1 Antibody (CL3929) [NBP2-59783] - Staining of U-2 OS cells showing specific staining of nuclear membrane in green. Microtubule and nuclear probes are visualized in red and blue, respectively (where available). Antibody staining is shown in green.
Immunohistochemistry-Paraffin: Lamin B1 Antibody (CL3929) [NBP2-59783]

Immunohistochemistry-Paraffin: Lamin B1 Antibody (CL3929) [NBP2-59783]

Immunohistochemistry-Paraffin: Lamin B1 Antibody (CL3929) [NBP2-59783] - Staining of human tonsil shows strong nuclear membrane immunoreactivity in lymphoid cells.
Immunohistochemistry-Paraffin: Lamin B1 Antibody (CL3929) [NBP2-59783]

Immunohistochemistry-Paraffin: Lamin B1 Antibody (CL3929) [NBP2-59783]

Immunohistochemistry-Paraffin: Lamin B1 Antibody (CL3929) [NBP2-59783] - Staining of human oral mucosa shows nuclear membrane positivity in the epithelial cells.
Immunohistochemistry-Paraffin: Lamin B1 Antibody (CL3929) [NBP2-59783]

Immunohistochemistry-Paraffin: Lamin B1 Antibody (CL3929) [NBP2-59783]

Immunohistochemistry-Paraffin: Lamin B1 Antibody (CL3929) [NBP2-59783] - Staining of human prostate shows nuclear membrane immunoreactivity in glandular epithelium cells.
Immunohistochemistry-Paraffin: Lamin B1 Antibody (CL3929) [NBP2-59783]

Immunohistochemistry-Paraffin: Lamin B1 Antibody (CL3929) [NBP2-59783]

Immunohistochemistry-Paraffin: Lamin B1 Antibody (CL3929) [NBP2-59783] - Staining of human small intestine shows nuclear membrane immunoreactivity in glandular and connective tissue cells.
Immunohistochemistry-Paraffin: Lamin B1 Antibody (CL3929) [NBP2-59783]

Immunohistochemistry-Paraffin: Lamin B1 Antibody (CL3929) [NBP2-59783]

Immunohistochemistry-Paraffin: Lamin B1 Antibody (CL3929) [NBP2-59783] - Staining of human testis shows strong nuclear membrane positivity in a subset of cells in the seminiferous tubules.

Applications for Lamin B1 Antibody (CL3929) - BSA Free

Application
Recommended Usage

Immunocytochemistry/ Immunofluorescence

2-10 ug/ml

Immunohistochemistry

1:500 - 1:1000

Immunohistochemistry-Paraffin

1:500 - 1:1000

Western Blot

1 ug/ml
Application Notes
For IHC-Paraffin, HIER pH 6 retrieval is recommended. ICC/IF Fixation Permeabilization: Use PFA/Triton X-100.

Reviewed Applications

Read 1 review rated 5 using NBP2-59783 in the following applications:

Formulation, Preparation, and Storage

Purification

Protein A purified

Formulation

PBS (pH 7.2) and 40% Glycerol

Format

BSA Free

Preservative

0.02% Sodium Azide

Concentration

1 mg/ml

Shipping

The product is shipped with polar packs. Upon receipt, store it immediately at the temperature recommended below.

Stability & Storage

Store at 4C short term. Aliquot and store at -20C long term. Avoid freeze-thaw cycles.

Background: Lamin B1

Nuclear lamins form a network of intermediate-type filaments at the nucleoplasmic site of the nuclear membrane.Two main subtypes of nuclear lamins can be distinguished, i.e. A-type lamins and B-type lamins. The A-type lamins comprise a set of three proteins arising from the same gene by alternative splicing, i.e. lamin A, lamin C and lamin Adel 10, while the B-type lamins include two proteins arising from two distinct genes, i.e. lamin B1 and lamin B2.

Alternate Names

ADLD, LMN2, LMNB1

Gene Symbol

LMNB1

Additional Lamin B1 Products

Product Documents for Lamin B1 Antibody (CL3929) - BSA Free

Certificate of Analysis

To download a Certificate of Analysis, please enter a lot or batch number in the search box below.

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Product Specific Notices for Lamin B1 Antibody (CL3929) - BSA Free

This product is for research use only and is not approved for use in humans or in clinical diagnosis. Primary Antibodies are guaranteed for 1 year from date of receipt.

Related Research Areas

Cancer Biomarkers
Epigenetics

Citations for Lamin B1 Antibody (CL3929) - BSA Free

Customer Reviews for Lamin B1 Antibody (CL3929) - BSA Free (1)

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  • Lamin B1 Antibody (CL3929)
    Name: Anonymous
    Application: Immunocytochemistry
    Sample Tested: fibroblasts
    Species: Human
    Verified Customer | Posted 11/07/2021
    staining in human fibroblasts
    Lamin B1 Antibody (CL3929) - BSA Free NBP2-59783

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Protocols

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FAQs for Lamin B1 Antibody (CL3929) - BSA Free

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  • Q: I'm looking for human lamin antibodies that might cross react with Drosophila lamins. I've found a few that I'm interested in, one of which comes in a smaller sample size. The other two do not have a smaller size ordering option. I was wondering if it would be possible to get these other antibodies in a smaller size as well, so we can test reactivity with Drosophila lamins before ordering the full-sized product?

    A:

    We have a wide range of antibodies to human Lamin. Unfortunately none of these has yet been tested against Drosophila samples, however you may be interested in our Innovator's Reward Program. This allows you to try our primary antibodies in an untested species or application, without the financial risk of failure. To participate you simply complete an online review with an image, detailing the positive or negative results of your study, and in return you receive a discount voucher for 100% of the purchase price of the reviewed product. We are unable to offer free samples of our antibodies but, as you rightly point out, some are available as smaller, sample size aliquots. If a sample size is not listed for a product I am afraid we are unable to offer a smaller aliquot than those already listed.

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